Imaging/Photomanipulation

成像/光处理

• 批准号: 8121504
• 负责人: Kenneth A Jacobson
• 金额: $ 32.27万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8121504
• 关键词: Binding; Biosensor; Cell Culture Techniques; Cell physiology; Cells; Chemicals; Data; Development; Event; Goals; Grant; Image; In Vitro; Lasers; Measurement; Measures; Mediating; Methods; Modeling; Molecular; Pattern; Process; Reagent; Research Personnel; Technology; Testing; Tissues; Work; cell motility; chromophore; migration; photoactivation; tissue/cell culture

The goal of the Imaging and Photomanipulation Initiative (I&PI) is to develop technologies for measuring and perturbing the localized activities that mediate and regulate cell migration and to use these technologies to make quantitative measurements for modeling cell migration. The emerging experimental paradigm is to initially detect and quantify local molecular events in migrating cells and then to manipulate such events by local activation or inactivation and assess the consequences on the migratory process. The Initiative has made great progress in developing the chemical and physical technologies and reagents to implement this paradigm. The goals for the next granting period are not only to continue developing these technologies, but also to use them on prototypical migration phenomena to develop working paradigms for studying cellular processes that are tightly regulated both spatially and temporally. The objectives of the I&PI are to: (1) develop and test, in cell cultures, in micro-patterned cell cultures, and tissues, new biosensors and probes both for local photoactivation and local photoinactivation, using chromophore assisted laser inactivation (CALI); (2) continue development of correlation methods for measuring molecular interactions, concentrations and dynamics both in vitro and in tissues; and, (3) use the advances provided in objectives (1) and (2) to develop data useful for modeling migration phenomena by investigators both inside and outside the Consortium.

成像和照相倡议的目的（I＆PI）是开发技术 测量和扰动调解和调节细胞迁移并使用这些局部活动 进行定量测量以建模细胞迁移的技术。新兴实验 范式最初检测和量化迁移细胞中的局部分子事件，然后操纵这种情况 通过局部激活或灭活事件，并评估对迁移过程的后果。倡议 在开发化学和物理技术和试剂中取得了重大进展来实施这一点 范例。下一个授予期的目标不仅是继续开发这些技术，而且是 还将它们用于原型迁移现象，以发展研究细胞的工作范例 在空间和时间上都受到严格调节的过程。 I＆PI的目标是：（1）发展 在细胞培养物，微图案细胞培养物以及组织中的测试，新的生物传感器和探针均用于局部 光活化和局部光激活，使用发色团辅助激光灭活（Cali）； （2）继续 开发用于测量分子相互作用，浓度和动力学的相关方法 体外和组织； （3）使用目标（1）和（2）中提供的进步来开发对 在财团内外，研究人员对迁移现象进行建模。