Imaging and Biosensors Core

成像和生物传感器核心

• 批准号: 7217766
• 负责人: Kenneth A Jacobson
• 金额: $ 39.19万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217766
• 关键词: bioimaging /biomedical imaging; biomedical facility; biosensor device; cell adhesion; cell migration; confocal scanning microscopy; fluorescence microscopy; fluorescence recovery after photobleaching; guanine nucleotide binding protein; guanine nucleotide exchange factors; immune response; inflammation; leukocytes; molecular /cellular imaging; photoactivation; vascular endothelium

The Imaging and Biosensor Core will provide specialized imaging techniques and fluorescence biosensors for the three component projects. Laser scanning and spinning disc confocal microscopy, multi-photon microscopy, multi-line total internal reflection fluorescence (TIRF) microscopy, FRAP, photoactivation and CALI (Chromophore Assisted Laser Inactivation) will be provided for the investigators. In addition, the Core will be able to provide traction force imaging as needed. Training of individuals from the component projects and advice on the design of imaging experiments will be provided. The core will also provide live cell biosensors for Rho family proteins and SGEF, and assist in their application. The Hahn lab has pioneered study of Rho family protein activation in living cells. Program project members will use published biosensors together with recently developed approaches that greatly enhance sensitivity and decrease perturbation of living cells. The core will also assist in application of biosensors developed by other laboratories. The Core Director has a long history of producing seminal developments in imaging methodogies (FRAP, single particle tracking, traction force imaging, and photoactivation and CALI as applied to cell adhesion and migration). Similarly, the Core Co-director has been a leader in the field of biosensor development (live cell indicators of Calmodulin-calcium binding, Rac, Cdc42 and Rho nucleotide state, MAP kinase phosphorylation, and fluorescent dyes for imaging of protein activity in vivo).

成像和生物传感器核心将提供专门的成像技术和荧光生物传感器 对于三个组成项目。激光扫描和转盘共焦显微镜，多光子 显微镜、多线全内反射荧光 (TIRF) 显微镜、FRAP、光活化和 将为研究人员提供 CALI（发色团辅助激光灭活）。此外， Core 将能够根据需要提供牵引力成像。对组件中的个人进行培训 将提供有关成像实验设计的项目和建议。 该核心还将为 Rho 家族蛋白和 SGEF 提供活细胞生物传感器，并协助它们 应用。 Hahn 实验室开创了活细胞中 Rho 家族蛋白激活的研究。程序 项目成员将使用已发表的生物传感器以及最近开发的方法，这些方法极大地 增强灵敏度并减少活细胞的干扰。该核心还将协助应用 其他实验室开发的生物传感器。 核心主任在成像方法（FRAP、 单粒子追踪、牵引力成像、光激活和 CALI 应用于细胞粘附 和迁移）。同样，核心联合总监一直是生物传感器开发领域的领导者 （钙调蛋白-钙结合的活细胞指标、Rac、Cdc42 和 Rho 核苷酸状态、MAP 激酶 磷酸化和用于体内蛋白质活性成像的荧光染料）。