Imaging/Photomanipulation

成像/光处理

• 批准号: 7195629
• 负责人: Kenneth A Jacobson
• 金额: $ 19万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7195629
• 关键词: bioimaging /biomedical imaging; biomedical facility; biosensor device; cell migration; chromophore; computer data analysis; cooperative study; image processing; imaging /visualization /scanning; microscopy; technology /technique development; time resolved data; tissue /cell culture

The goal of the Imaging and Photomanipulation Initiative (I&PI) is to develop technologies for measuring and perturbing the localized activities that mediate and regulate cell migration and to use these technologies to make quantitative measurements for modeling cell migration. The emerging experimental paradigm is to initially detect and quantify local molecular events in migrating cells and then to manipulate such events by local activation or inactivation and assess the consequences on the migratory process. The Initiative has made great progress in developing the chemical and physical technologies and reagents to implement this paradigm. The goals for the next granting period are not only to continue developing these technologies, but also to use them on prototypical migration phenomena to develop working paradigms for studying cellular processes that are tightly regulated both spatially and temporally. The objectives of the I&PI are to: (1) develop and test, in cell cultures, in micro-patterned cell cultures, and tissues, new biosensors and probes both for local photoactivation and local photoinactivation, using chromophore assisted laser inactivation (CALI); (2) continue development of correlation methods for measuring molecular interactions, concentrations and dynamics both in vitro and in tissues; and, (3) use the advances provided in objectives (1) and (2) to develop data useful for modeling migration phenomena by investigators both inside and outside the Consortium.

成像和光操控计划 (I&PI) 的目标是开发技术 测量和扰乱介导和调节细胞迁移的局部活动并使用这些 为细胞迁移建模进行定量测量的技术。新兴实验 范式是首先检测和量化迁移细胞中的局部分子事件，然后操纵这些事件 局部激活或失活事件并评估对迁移过程的影响。该倡议 在开发实现这一目标的化学和物理技术和试剂方面取得了巨大进展 范例。下一个资助期的目标不仅是继续开发这些技术，而且 还将它们用于原型迁移现象，以开发研究细胞的工作范式 在空间和时间上受到严格调控的过程。 I&PI 的目标是： (1) 开发 并在细胞培养物、微图案细胞培养物和组织中测试新的生物传感器和探针，用于局部 使用发色团辅助激光灭活（CALI）进行光活化和局部光灭活； (2) 继续 开发用于测量分子相互作用、浓度和动态的相关方法 体外和组织内； (3) 利用目标 (1) 和 (2) 中提供的进展来开发有用的数据 由联盟内部和外部的调查人员对迁移现象进行建模。