High Performance NMR uFlowProbe Optimized for Mass-Sensitive Investigation of Wel

高性能 NMR uFlowProbe 针对井的质量敏感研究进行了优化

• 批准号: 7909754
• 负责人: TIMOTHY L PECK
• 金额: $ 9.96万
• 依托单位: PROTASIS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7909754
• 关键词: Acetone; Adhesions; Air; Architecture; Benzene; Biological; Categories; Chemicals; Communication; Copper; Data Quality; Detection; Development; Drug Industry; Electromagnetic Fields; Encapsulated; Environment; Evaluation; Family; Fluorine; Frequencies; Funding; Generations; Goals; Health; Height; Human; Individual; Industry; Injection of therapeutic agent; Investigation; Laboratories; Liquid Chromatography; Liquid substance; Magnetism; Maintenance; Measurement; Mechanics; Methodology; Minor; Molds; Performance; Pharmacologic Substance; Phase; Predisposition; Process; Property; Protons; RF coil; Relaxation; Reporting; Research; Research Personnel; Resources; Role; Sampling; Signal Transduction; Small Business Innovation Research Grant; Solid; Techniques; Technology; Teflon; Temperature; Time; Universities; Vendor; Work; base; computerized data processing; cost; design; interest; meetings; phase 1 study; public health relevance; research study; success

DESCRIPTION (provided by applicant): The impact over the past five years of the development of microliter-volume, highly mass-sensitive NMR detection probes has been substantial. Combining sensitivity, simplicity, and economy, these room-temperature probes require very minor initial shimming and virtually no shimming or tuning adjustments between individual samples, and do not carry the high cost or maintenance requirements of cryogenically-cooled probe technologies while providing comparable mass sensitivity. As such, they are ideally suited to the analysis of low-abundance analytes typical of liquid chromatography fractions. The proposed effort now offers to extend this product capability to include 19F detection, a technique that is of great importance to university research and to pharmaceutical and other chemical product industries due to the inherent chemical dispersion that 19F offers in terms of chemical shift and due to its natural absence in biological samples. This proposal specifically seeks funding to develop a new category of probe that can accomplish both 1H and 19F detection (using a single probe) to obtain background-free, quality data. A favored approach of this proposal utilizes a unique dual flowcell architecture owned by Protasis to essentially incorporate two separately-optimized probe functionalities into a single mechanical probe package. The potential impact on human health resulting from new generations of economical and practical NMR-based analyzers is extremely high. PUBLIC HEALTH RELEVANCE: This proposal seeks funding to develop a new category of mass-sensitive probe that can accomplish both 1H and 19F detection (using a single probe) to obtain background-free, quality data from low-abundance analytes, e.g. fractions originating from liquid chromatography. Research groups asking for this new generation of high performance NMR detection technology include the pharmaceutical industry, who require an economically-justifiable means to include 19F detection in discovery and development efforts using smaller amounts of sample than was available in the past, and academic researchers who in addition to economy also wish to realize the performance advantages of working at smaller size scale. The potential impact on human health resulting from new generations of economical and practical NMR-based analyzers is extremely high.

描述（由申请人提供）：在过去五年中，微胶卷 - 高度敏感的NMR检测探针的影响很大。这些室温探针结合了敏感性，简单性和经济性，需要非常小的初始杂质，并且几乎没有单个样品之间的杂音或调整调整，并且在提供可比的质量敏感性的同时，不承担高成本或维护的高成本或维护要求。因此，它们非常适合分析液态色谱分数典型的低丰度分析物。现在，拟议的努力旨在扩展该产品能力，以包括19F检测，这是该技术对大学研究以及制药和其他化学产品行业非常重要的技术，这是由于19F在化学转移方面提供了固有的化学分散体，并且由于其在生物样品中的自然缺失。该提案专门寻求资金来开发一个可以完成1H和19F检测（使用单个探针）以获取无背景，质量数据的新的探针类别。该提案的一种偏爱方法利用Protasis拥有的独特双流循环结构，将两个单独优化的探针功能纳入一个机械探针包中。新一代经济和实用的基于NMR的分析仪对人类健康的潜在影响极高。 公共卫生相关性：该提案寻求资金来开发一种新类别的质量敏感探测器，这些探针可以完成1H和19F检测（使用单个探针），以获取低含量分析物的无背景，质量数据，例如源自液相色谱的馏分。询问新一代高性能NMR检测技术的研究小组包括制药行业，他们需要一种经济上可行的手段，将19F检测包括在发现和开发工作中，使用较少的样本与过去相比，他们除了经济外，还希望实现在尺寸较小的规模上的绩效优势。新一代经济和实用的基于NMR的分析仪对人类健康的潜在影响极高。