Microcoil-based Probe for Protein NMR

用于蛋白质 NMR 的微线圈探针

• 批准号: 6833284
• 负责人: TIMOTHY L PECK
• 金额: $ 9.98万
• 依托单位: PROTASIS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833284
• 关键词: bioengineering /biomedical engineering; biomedical equipment development; carbon; dielectric property; fluid flow; hydrogen ions; molecular probes; nitrogen; nuclear magnetic resonance spectroscopy; polymers; protein structure; temperature

DESCRIPTION (provided by applicant): The goal of this effort is the creation of a new line of microcoil-based NMR detection probes specifically targeted to protein analysis. The submitting organization will utilize a patented and exclusively licensed approach to susceptibility matching that has enabled small molecule analysis using microcoil-based probes. The fundamental contribution of microcoils to protein analysis is the ability to acquire data using less sample, micrograms rather than milligrams. This provides the protein chemist with an NMR measurement solution that generally saves time by requiring less expressed protein, and that accommodates expression techniques that are of potentially high value but potentially lower yield. The probes developed under this SBIR will be designed to interface to standard NMR spectrometers ranging in (proton) frequency from 400 MHz - 750 MHz, and to sample management and analytical separations technologies both in existence and under development in the rapidly growing field of proteomics. The immediate market will be the pharmaceutical and medicinal chemistry industries.

描述（由申请人提供）：这项工作的目的是创建针对蛋白质分析的针对蛋白质分析的新系列NMR检测探针。提交的组织将利用专利且仅具有许可的方法来匹配敏感性，从而实现了使用基于微型机油的探针进行小分子分析。微层对蛋白质分析的基本贡献是使用较少的样品，微克而不是毫克获取数据的能力。这为蛋白质化学家提供了NMR测量溶液，该溶液通常通过需要较少表达的蛋白质节省时间，并适应潜在的高值但潜在较低产量的表达技术。在此SBIR下开发的探针将被设计为与（Proton）频率在400 MHz -750 MHz（质子）频率中的标准NMR光谱仪的接口，以及在蛋白质组学快速生长的领域中存在的样品管理和分析分离技术。直接市场将是药物和药物化学行业。