Genetic Biochemical Studies of Plant Steroid Signaling

植物类固醇信号传导的遗传生化研究

• 批准号: 7993689
• 负责人: ZHIYONG WANG
• 金额: $ 16.14万
• 依托单位: CARNEGIE INSTITUTION OF WASHINGTON, D.C.
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7993689
• 关键词: 14-3-3 Proteins; Affect; Agriculture; Animals; Arabidopsis; Binding; Biochemical; Biological; Biological Models; Cell Communication; Cell Nucleus; Cell membrane; Cell surface; Cells; DNA Binding; DNA-Binding Proteins; Development; Developmental Process; Disease; Funding; Gene Expression; Gene Targeting; Genes; Genetic; Genetic Transcription; Genomics; Goals; Growth; Growth Factor; Growth and Development function; Health; Homologous Gene; Hormonal; Hormones; Human; Investigation; Laboratories; Location; Malignant Neoplasms; Mediating; Membrane; Nuclear; Nuclear Receptors; Organ; Organism; Pathway interactions; Phenotype; Phosphorylation; Phosphorylation Site; Phosphotransferases; Physiological; Plant Growth Regulators; Plants; Play; Proteins; Proteolysis; Proteomics; Regulation; Role; Signal Transduction; Signal Transduction Pathway; Signaling Protein; Specificity; Steroids; cell type; chemical genetics; chromatin immunoprecipitation; mutant; non-genomic; novel; plant growth/development; programs; receptor; receptor binding; research study; response; steroid hormone; transcription factor

DESCRIPTION (provided by applicant): Signaling by hormones and growth factors is an essential mechanism of growth regulation in multicellular organisms. Steroids are unique hormones that regulate important physiological and developmental processes in both plants and animals. Brassinosteroids (BRs), the plant steroid hormone with essential roles, share structural and functional similarities with animal steroid hormones but act through a distinct signaling mechanism. While animal steroid hormones act either through nuclear receptor transcription factors to regulate gene expression or through membrane-bound receptors to activate non-genomic responses, BRs bind to a receptor kinase at the cell surface and regulate nuclear gene expression through a signal transduction cascade. Downstream BR signaling involves both ancient signaling proteins, such as GSK3-like kinases and 14-3-3 proteins, and plant specific DNA binding proteins. My laboratory uses Arabidopsis as the model system to understand how BR regulates plant growth and development at the biochemical, cell biological, and physiological levels. We have studied how BRs regulate gene expression by controlling the phosphorylation status and activity of the key transcription factor BZR1, and we have demonstrated how BZR1 regulates the expression of BR-responsive genes. We plan to continue our study of the BR signal transduction pathway using genetic and biochemical approaches, and we plan to study how the BR signal transduction pathway is connected to various developmental processes as well as to other hormonal pathways that regulate common developmental processes. This study will advance our understanding of plant growth regulation as well as steroid signaling in general. Because steroid hormones are involved in many major diseases including cancer in human, this study will have broad implications for both agriculture and human health.

描述（由申请人提供）：激素和生长因子的信号传导是多细胞生物生长调节的基本机制。类固醇是独特的激素，可调节动植物中重要的生理和发育过程。黄铜固醇（BRS）是具有基本作用的植物类固醇激素，与动物类固醇激素具有结构和功能相似性，但通过独特的信号机制作用。虽然动物类固醇激素要么通过核受体转录因子起作用以调节基因表达或通过膜结合的受体激活非基因组反应，但BRS与细胞表面的受体激酶结合并通过信号转导级联来调节核基因表达。下游BR信号传导既涉及古代信号蛋白，例如GSK3样激酶和14-3-3蛋白，以及植物特定的DNA结合蛋白。我的实验室使用拟南芥作为模型系统，以了解BR如何调节生化，细胞生物学和生理水平的植物生长和发育。我们已经研究了BRS如何通过控制关键转录因子BZR1的磷酸化状态和活性来调节基因表达，并证明了BZR1如何调节BR响应基因的表达。我们计划使用遗传和生化方法继续研究BR信号转导途径，并计划研究BR信号转导途径如何连接到各种发育过程以及调节常见发育过程的其他激素途径。这项研究将提高我们对植物生长调节以及类固醇信号的理解。由于类固醇激素参与包括人类癌症在内的许多主要疾病，因此这项研究将对农业和人类健康具有广泛的影响。