PROTEOMIC STUDY OF PROTEIN MODIFICATIONS IN PLANTS

植物中蛋白质修饰的蛋白质组学研究

• 批准号: 8363847
• 负责人: ZHIYONG WANG
• 金额: $ 0.46万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363847
• 关键词: Acetylation; Arabidopsis; Environment; Enzymes; Funding; Goals; Grant; Growth; Hormones; Mass Spectrum Analysis; Modification; National Center for Research Resources; Neurons; Peptides; Phosphorylation; Plants; Play; Post-Translational Protein Processing; Post-Translational Regulation; Principal Investigator; Process; Proteomics; Regulation; Research; Research Infrastructure; Resources; Role; Sampling; Signal Transduction; Source; System; United States National Institutes of Health; cell growth regulation; cost; glycosylation; mutant; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to analyze protein modifications in plants using mass spectrometry. Posttranslational modifications, such as glycosylation, phosphorylation, and acetylation, play important roles in cellular regulation. Because plants are immobile and lack neuron systems, responses to the changing environment relies on cellular regulatory mechanisms. Therefore, plants are believed to have more sophisticate posttranslational regulation systems, and thus good system for proteomic study of cellular regulation mechanisms. In this project, posttranslationally modified peptides will be enriched from plant samples and analyzed by mass spectrometry. The study will focus on glycosylation (O-Gluc-NAc) and acetylation, which are known to be important for hormone signaling and growth regulation in plants. Arabidopsis mutants for enzymes catalyzing these modification processes will be used as references in the studies.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 该项目的目的是使用质谱法分析植物中的蛋白质修饰。翻译后修饰，例如糖基化，磷酸化和乙酰化，在细胞调节中起重要作用。由于植物是不动的，缺乏神经元系统，因此对环境变化的反应依赖于细胞调节机制。因此，据信植物具有更复杂的翻译后调节系统，因此具有良好的细胞调节机制研究系统。在这个项目中，翻译后修饰的肽将从植物样品中富集并通过质谱分析。该研究将集中于糖基化（O-Gluc-NAC）和乙酰化，这对于植物的激素信号传导和生长调节很重要。用于催化这些修饰过程的酶的拟南芥突变体将用作研究中的参考。