FUNCTIONAL STUDIES OF BRASSINOSTEROID-REGULATED PROTEINS

油菜素类固醇调节蛋白的功能研究

• 批准号: 8363848
• 负责人: ZHIYONG WANG
• 金额: $ 0.13万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363848
• 关键词: Affinity Chromatography; Arabidopsis; Binding; Blood Vessels; Cell Surface Receptors; Cell division; Cell membrane; Cells; Developmental Process; Disease Resistance; Funding; Gene Expression; Genetic; Germination; Grant; Mass Spectrum Analysis; Molecular; Molecular Genetics; National Center for Research Resources; Nuclear; Phosphotransferases; Physiological Processes; Plants; Play; Principal Investigator; Productivity; Proteins; Proteomics; Research; Research Infrastructure; Resources; Role; Seeds; Signal Pathway; Signal Transduction; Source; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; cost; gene function; improved; receptor; steroid hormone; stress tolerance; success; tool; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Brassinosteroids (BRs) are plant steroid hormones, which play important roles in a wide range of physiological and developmental processes, including cell expansion, cell division, vascular differentiation, seed germination, photomorphogenesis, stress tolerance and disease resistance. Molecular genetic studies have identified several BR signal transduction components of the BR signaling pathway, through which BRs bind the cell surface receptors to regulate nuclear gene expression. However, there are still gaps in the current BR signaling pathway. In particular, the substrates of the receptor kinases that transduce the BR signal from plasma membrane to cytoplasmic components remain unknown. Despite the success of genetic studies, many genes' function cannot be discovered by forward genetic approaches, largely due to genetic redundancy. Therefore, alternative approaches are required to significantly advance our understanding of the BR signaling pathway. Thus, we have performed proteomic studies and identified BR-regulated proteins in Arabidopsis using two-dimensional difference gel electrophoresis (2-D DIGE) followed by mass spectrometry. This study aims at characterizing plasma membrane-localized BR-responsive proteins, including the BR-signaling kinases (BSKs). Functional studies of these proteins have demonstrated their key role as BRI1 substrate in the BR signaling pathway. We plan to identify additional interacting proteins of BSKs using affinity purification followed by mass spectrometry analysis. Such analysis will advance our understanding of BR signal transduction and provide molecular tools for improving plant productivity.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 黄铜固醇（BRS）是植物类固醇激素，它们在各种生理和发育过程中起着重要作用，包括细胞扩张，细胞分裂，血管分化，种子发芽，光生形态发生，胁迫耐受性和抗病性。分子遗传研究已经确定了BR信号通路的几种BR信号转导成分，BRS通过该途径结合细胞表面受体以调节核基因表达。但是，当前BR信号通路中仍然存在差距。特别是，将BR信号从质膜传递到细胞质成分的受体激酶的底物仍然未知。尽管遗传研究取得了成功，但许多基因的功能无法通过正向遗传方法发现，这主要是由于遗传冗余。因此，需要替代方法来显着提高我们对BR信号通路的理解。因此，我们已经进行了蛋白质组学研究，并使用二维差异凝胶电泳（2-D DIGE）随后进行了质谱法，并使用二维差异质量差鉴定了拟南芥中BR调节的蛋白质。这项研究旨在表征质膜 - 定位的BR响应蛋白，包括BR信号激酶（BSK）。 这些蛋白质的功能研究表明，它们在BR信号传导途径中作为BRI1底物的关键作用。我们计划使用亲和力纯化，然后进行质谱分析来鉴定BSK的其他相互作用蛋白。这种分析将提高我们对BR信号转导的理解，并提供提高植物生产率的分子工具。