Rapid Scan Biomedical EPR Spectroscopy and Imaging

快速扫描生物医学 EPR 光谱和成像

• 批准号: 7870319
• 负责人: Gareth R Eaton
• 金额: $ 55.92万
• 依托单位: UNIVERSITY OF DENVER (COLORADO SEMINARY)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7870319
• 关键词: Affect; Air; Animals; Biomedical Research; Chicago; Computer software; Computers; Detection; Digital Signal Processing; Electron Spin Resonance Spectroscopy; Electrons; Engineering; Free Radicals; Frequencies; Image; Industry; Measures; Methodology; Methods; Mus; Noise; Operating System; Oxygen; Oxygen saturation measurement; Penetration; Physiologic pulse; Physiological; Physiology; Process; Relative (related person); Relaxation; Research; Research Personnel; Sampling; Scanning; Scientist; Signal Transduction; Source; Spectrum Analysis; System; Technology; Testing; Time; Universities; absorption; analog; data acquisition; design; digital; experience; improved; in vivo; innovation; instrumentation; magnetic field; oncology; rapid detection; research study; response; software systems; tumor

DESCRIPTION (provided by applicant): A new and innovative electron paramagnetic resonance (EPR) methodology will be developed for in vivo spectroscopy, imaging and biomedical research. A partnership of engineers, research scientists, clinicians, and industry propose to design and build rapid-scan EPR systems operating at 250 MHz and at L-band (ca. 1.2 GHz). The emphasis is on careful engineering of the spectrometer systems, including magnet, scan coils, resonator, and data acquisition to optimize physiological EPR signal acquisition for in vivo spectroscopy and imaging at the low RF frequencies that are accepted as the best compromises between penetration depth and signal to noise for in vivo animal studies. Rapid-scan EPR encompasses the regime in which the magnetic field sweep is fast relative to relaxation times, which is a newly developed intermediate regime between CW and pulsed EPR. Direct-detection rapid-scan EPR signals provide the absorption lineshape directly, reveal electron spin relaxation times without requiring high incident power, and provide accurate relative amplitudes of peaks in rapidly decaying signals. The Specific Aims are: (1) Build a dedicated rapid-scan spectrometer at L-band (ca. 1.2 GHz) with scan rates optimized for biomedical, in vivo, and imaging experiments. (2) At 250 MHz build improved resonators, rapid-scan coils and drivers, and optimize the rapid-scan bridge. (3) Build a 250 MHz rapid-scan bridge, resonator, and magnetic field scan coil unit at the University of Denver and install it at the University of Chicago Center for In Vivo Imaging of Physiology, where it will be used to image oxygen concentrations in animal tumors. The benefits of traditional CW imaging, pulsed EPR imaging, and rapid-scan imaging for oximetry and oncology will be compared. (4) Design, build and test hardware/software systems for acquisition of the rapid-scan signal and post-processing of spectral information that will be used at 250 MHz and at L-band.

描述（由申请人提供）：将为体内光谱，成像和生物医学研究开发一种新的和创新的电子顺磁共振（EPR）方法。工程师，研究科学家，临床医生和行业的合作伙伴关系提议设计和建立在250 MHz和L波段（约1.2 GHz）运行的Rapid-Scan EPR系统。重点放在光谱仪系统的仔细工程上，包括磁铁，扫描线圈，谐振器和数据获取，以优化体内光谱的生理EPR信号获取和在低RF频率下的成像，这些频率被接受为渗透深度和信号的最佳损害与噪声之间的最佳损害。 Rapid-Scan EPR涵盖了磁场扫描相对于松弛时间快速的状态，这是CW和脉冲EPR之间新开发的中间方案。直接检测快速扫描EPR信号可直接提供吸收线形，揭示电子自旋松弛时间而无需高入射力，并在快速衰减的信号中提供准确的峰值相对振幅。具体目的是：（1）在L波段（Ca. 1.2 GHz）上建立一个专用的快速扫描光谱仪，并针对生物医学，体内和成像实验进行了优化的扫描速率。 （2）在250 MHz时，建立了改进的谐振器，快速扫描线圈和驱动器，并优化了快速扫描的桥梁。 （3）在丹佛大学建造一个250 MHz快速扫描的桥，谐振器和磁场扫描线圈单元，并在芝加哥大学生理学中的芝加哥大学体内成像中心进行安装，并将其用于在动物肿瘤中图像氧气浓度。将比较传统CW成像，脉冲EPR成像以及对血氧仪和肿瘤学的快速扫描成像的好处。 （4）设计，构建和测试硬件/软件系统，以获取将在250 MHz和L波段使用的光谱信息的快速扫描信号和后处理。