In Vivo EPR Bioengineering Research Partnership

体内 EPR 生物工程研究合作伙伴

• 批准号: 6344154
• 负责人: Gareth R Eaton
• 金额: $ 28.95万
• 依托单位: UNIVERSITY OF DENVER (COLORADO SEMINARY)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6344154
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; computer system design /evaluation; electron spin resonance spectroscopy; free radicals; interdisciplinary collaboration; laboratory mouse; magnetic field; mathematical model; model design /development

DESCRIPTION (provided by applicant): In vivo paramagnetic resonance (EPR) requires optimal signal-to-noise (S/N) enhancement in the shortest possible time. To study radicals in deep tissues it is necessary to perform the EPR measurements at low radiofrequency (e.g., 250 MHz), as in MRI. This decreases the S/N relative to the more common 9 GHz EPR. Physiological motions and metabolism occurring within the time of the usual EPR measurements necessitate the development of special techniques for in vivo studies. It is proposed to establish a partnership of engineers, research scientists, clinicians, and industry to fully engineer an EPR system dedicated to in vivo spectroscopy and imaging. As a first step, it is proposed to engineer a CW 250 MHz EPR spectrometer system optimized for the best in vivo free radical sensitivity per unit time. The specific tasks include the design, construction, and testing of an air-core magnet for in vivo EPR optimized for rapid magnetic field scans, and a control system for scanning the magnetic field rapidly. We introduce the innovation that the magnet will be resonated, and magnetic field scans will be sinusoidal. Measurement of the noise spectral densities of the spectrometer system, and of a spectrometer with a mouse in the resonator, will provide the basis for a mathematical model of the spectrometer noise characteristics from which one can predict the S/N per unit time expected for various magnetic field scan rates. The S/N for various scan rates will be compared with the predicted values. Software will be written to linearize and deconvolute the spectral information recorded under rapid-scan conditions. In subsequent effort it is proposed to extend the scope of the bioengineering research partnership to tackle the problems of optimal compensation for physiological motion, acquisition of the full RF spectrum and post-processing to replace analog pre-processing, and design of open magnet structures to achieve better patient acceptance and decrease costs.

描述（由申请人提供）：体内顺磁共振（EPR） 需要最短的最佳信噪比（S/N）增强功能 时间。要在深组织中研究自由基，有必要执行EPR 低射频（例如250 MHz）处的测量值如MRI。这减少了 S/N相对于更常见的9 GHz EPR。生理运动和 需要在通常的EPR测量之时发生代谢 用于体内研究的特殊技术的发展。提议 建立工程师，研究科学家，临床医生和 行业旨在完全设计一个专门用于体内光谱和的EPR系统 成像。第一步，提议设计CW 250 MHz EPR 光谱仪系统优化，以最佳体内自由基灵敏度 单位时间。具体任务包括设计，构造和测试 用于快速磁场扫描的体内EPR的空气核磁铁， 以及用于快速扫描磁场的控制系统。我们介绍 磁铁将引起共鸣的创新，磁场扫描将是 正弦。测量光谱仪的噪声光谱密度 系统，以及带有谐振器中鼠标的光谱仪，将提供 来自光谱仪噪声特性的数学模型的基础 哪一个可以预测各种磁场的单位时间S/N 扫描率。将各种扫描率的S/N与预测的 值。软件将被写成线性化和反应频谱 在快速扫描条件下记录的信息。在随后的努力中 提议将生物工程研究合作伙伴关系的范围扩展到 解决生理运动的最佳补偿问题， 获取完整的RF光谱和后期处理以取代模拟 预处理和设计开放式磁铁结构以实现更好的患者 接受并降低成本。