Rapid Scan Biomedical EPR Spectroscopy and Imaging

快速扫描生物医学 EPR 光谱和成像

• 批准号: 7434674
• 负责人: Gareth R Eaton
• 金额: $ 49.43万
• 依托单位: UNIVERSITY OF DENVER (COLORADO SEMINARY)
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-06 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7434674
• 关键词: Affect; Air; Animals; Biological Process; Biomedical Research; Chicago; Computer software; Computers; Detection; Digital Signal Processing; Disease; Electron Spin Resonance Spectroscopy; Electrons; Engineering; Free Radicals; Frequencies; Future; Image; Industry; Investigation; Measurement; Measures; Methodology; Methods; Mus; Noise; Operating System; Oxygen; Oxygen saturation measurement; Peripheral Vascular Diseases; Physiologic pulse; Physiological; Physiology; Process; Pulse taking; Rate; Relative (related person); Relaxation; Research; Research Personnel; Sampling; Scanning; Scientist; Signal Transduction; Source; Spectrum Analysis; Spin Trapping; System; Technology; Testing; Time; Universities; Wound Healing; absorption; cancer therapy; data acquisition; design; digital; experience; improved; in vivo; innovation; instrumentation; magnetic field; oncology; rapid detection; research study; response; software systems; tumor

A new and innovative electron paramagnetic resonance (EPR) methodology will be developed for in vivo spectroscopy, imaging and biomedical research. A partnership of engineers, research scientists, clinicians, and industry propose to design and build rapid-scan EPR systems operating at 250 MHz and at L-band (ca. 1.1 GHz). The emphasis is on careful engineering of the spectrometer systems, including magnet, scan coils, resonator, and data acquisition to optimize physiological EPR signal acquisition for in vivo spectroscopy and imaging. Rapid-scan EPR encompasses the regime in which the magnetic field sweep is fast relative to relaxation times, which is a newly developed intermediate regime between CW and pulsed EPR. Direct-detection rapid-scan EPR signals provide the absorption lineshape directly, reveal electron spin relaxation times without requiring high incident power, and provide accurate relative amplitudes of peaks in rapidly decaying signals. The Specific Aims are: (1) Build a dedicated rapid-scan spectrometer at L-band (ca. 1.1 GHz) with scan rates optimized for biomedical, in vivo, and imaging experiments. (2) Build improved resonators, rapid scan coils and drivers, and dedicated rapid-scan bridge at 250 MHz. (3) Build a 250 MHz rapid scan bridge, resonator, and magnetic field scan coil unit at the University of Denver and install it at the University of Chicago Center for In Vivo Imaging of Physiology, where it will be used to image oxygen concentrations in animal tumors. The benefits of traditional CW imaging, pulsed EPR imaging, and rapid-scan imaging for oximetry and oncology will be compared. (4) Design, build and test hardware/software systems for acquisition of the rapid-scan signal and post-processing of spectral information that will be used at 250 MHz and at L-band. In addition to in vivo imaging, future applications include the study of transient paramagnetic species, such as spin-trapped radicals with short lifetimes, time-dependent biological processes, and fundamental spin relaxation phenomena. This instrumentation is a new enabling technology and will create a new era for measuring oxygen in vivo and for scientific investigation of paramagnetic species, including in vivo free radicals. Free radicals are implicated in many diseases, and measurement of oxygen in vivo is important for cancer treatment, peripheral vascular disease, and wound healing.

将为体内开发一种新的和创新的电子顺磁共振（EPR）方法论 光谱，成像和生物医学研究。工程师，研究科学家，临床医生的伙伴关系， 行业建议设计和建立在250 MHz和L波段运行的快速扫描EPR系统（约。 1.1 GHz）。重点是光谱仪系统的仔细工程，包括磁铁，扫描 线圈，谐振器和数据采集，以优化体内生理EPR信号获取 光谱和成像。 Rapid-Scan EPR涵盖了磁场扫描为 相对于放松时间快速，这是CW和脉冲之间的新开发的中间状态 EPR。直接检测快速扫描EPR信号可直接提供吸收线形，揭示电子自旋 放松时间不需要高入射力，并提供准确的相对幅度 迅速腐烂的信号。 具体目的是：（1）在L波段（约1.1 GHz）构建专用的快速扫描光谱仪 针对生物医学，体内和成像实验优化的速率。 （2）建立改进的谐振器，快速扫描 线圈和驱动器，以及250 MHz的专用快速扫描桥。 （3）建造250 MHz快速扫描桥， 丹佛大学的谐振器和磁场扫描线圈单元，并将其安装在大学 芝加哥生理学中心成像中心，将其用于图像氧气浓度 动物肿瘤。传统CW成像，脉冲EPR成像和快速扫描成像的好处 将比较血氧和肿瘤学。 （4）设计，构建和测试硬件/软件系统 采购快速扫描信号和光谱信息的后处理，该信息将在250 MHz处使用 在L波段。除了体内成像外，未来的应用还包括瞬态顺磁性的研究 物种，例如寿命短的自旋自由基，时间依赖于时间依赖的生物过程和 基本的自旋松弛现象。该仪器是一种新的促成技术，将创建一个 测量体内氧气和对顺磁性物种的科学研究的新时代，包括 体内自由基。自由基与许多疾病有关，体内氧的测量是 对于癌症治疗，周围血管疾病和伤口愈合至关重要。