USING ANNOTATED PEPTIDE MASS SPECTRUM LIBRARIES FOR PROTEIN IDENTIFICATION

使用带注释的肽质谱库进行蛋白质鉴定

• 批准号: 8169143
• 负责人: RONALD C. BEAVIS
• 金额: $ 0.12万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169143
• 关键词: Charge; Computer Retrieval of Information on Scientific Projects Database; Computer software; Databases; Funding; Grant; Homo sapiens; House mice; Institution; Ions; Libraries; Mass Spectrum Analysis; Modification; Parents; Peptides; Proteins; Proteome; Research; Research Personnel; Resources; Saccharomyces cerevisiae; Source; System; United States National Institutes of Health; base; protein aminoacid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 描述了一个用于创建用相应肽序列注释的串联质谱库的系统。该系统基于当前在全球蛋白质机器数据库（GPMDB）中可用的注释光谱。图书馆光谱是通过将用相同的肽序列，序列修饰和父离子电荷进行注释的光谱来创建的。构建了库，以便可以将实验性肽串联质量光谱与图书馆中的谱系进行比较，从而基于评分实验光谱与文库内容的评分，从而导致肽序列识别。构建了执行此类库搜索的软件实现，并成功地用于获得序列标识。与Homo Sapiens，Mus Musculus和Saccharomyces酿酒酵母蛋白质组织和搜索软件的注释的串联质谱库可供其他组下载和使用。