USING ANNOTATED PEPTIDE MASS SPECTRUM LIBRARIES FOR PROTEIN IDENTIFICATION

使用带注释的肽质谱库进行蛋白质鉴定

• 批准号: 8361521
• 负责人: RONALD C. BEAVIS
• 金额: $ 0.13万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361521
• 关键词: Charge; Computer software; Databases; Funding; Grant; Homo sapiens; House mice; Ions; Libraries; Mass Spectrum Analysis; Methods; Modification; National Center for Research Resources; Parents; Peptides; Principal Investigator; Proteins; Proteome; Research; Research Infrastructure; Resources; Saccharomyces cerevisiae; Source; System; United States National Institutes of Health; base; cost; macromolecule; protein aminoacid sequence

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. A system for creating a library of tandem mass spectra annotated with corresponding peptide sequences was described. This system was based on the annotated spectra currently available in the Global Proteome Machine Database (GPMDB). The library spectra were created by averaging together spectra that were annotated with the same peptide sequence, sequence modifications, and parent ion charge. The library was constructed so that experimental peptide tandem mass spectra could be compared with those in the library, resulting in a peptide sequence identification based on scoring the similarity of the experimental spectrum with the contents of the library. A software implementation that performs this type of library search was constructed and successfully used to obtain sequence identifications. The annotated tandem mass spectrum libraries for the Homo sapiens, Mus musculus, and Saccharomyces cerevisiae proteomes and search software were made available for download and use by other groups. These and other advances are discussed in: Mass spectrometric protein identification using the global proteome machine. Feny¿ D, Eriksson J, Beavis R. Methods Mol Biol. 2010;673:189-202

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 描述了一个用于创建用相应肽序列注释的串联质谱库的系统。该系统基于当前在全球蛋白质机器数据库（GPMDB）中可用的注释光谱。该库是通过将用相同肽序列，序列修饰和父离子电荷的平均谱图组合在一起而创建的。构建了库，以便可以将实验性肽串联质量光谱与图书馆中的谱系进行比较，从而基于评分实验光谱与文库内容的评分，从而导致肽序列识别。构建了执行此类库搜索的软件实现，并成功地用于获得序列标识。与Homo Sapiens，Mus Musculus和Saccharomyces酿酒酵母蛋白质组织和搜索软件的注释的串联质谱库可供其他组下载和使用。这些进步和其他进步在以下讨论中： 使用全球蛋白质组机器鉴定质谱蛋白。 芬尼D，Eriksson J，Beavis R. 方法摩尔生物。 2010; 673：189-202