IMAGING AND HISTOLOGY CORE

成像和组织学核心

基本信息

批准号：
8167594
负责人：
SCOTT Allen WETZEL
金额：
$ 10.07万
依托单位：
UNIVERSITY OF MONTANA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-01 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8167594
关键词：
Biological Computer Retrieval of Information on Scientific Projects Database Computer software Computers Consult Core Facility Cultured Cells Equipment Equipment and supply inventories Experimental Designs Freezing Funding Grant Histology Image Image Analysis Imagery Immunohistochemistry Institution Light Light Microscope Maintenance Microscope Microtome - medical device Molecular Montana Optics Paraffin Embedding Proteins Research Research Personnel Resources Scientist Services Slide Source Staining method Stains Students System Techniques Tissues Training United States National Institutes of Health Universities cellular imaging charge coupled device camera experience finesse fluorescence imaging human tissue immunocytochemistry meetings professor programs tissue culture tissue fixing tissue preparation tool

Biological Computer Retrieval of Information on Scientific Projects Database Computer software Computers Consult Core Facility Cultured Cells Equipment Equipment and supply inventories Experimental Designs Freezing Funding Grant Histology Image Image Analysis Imagery Immunohistochemistry Institution Light Light Microscope Maintenance Microscope Microtome - medical device Molecular Montana Optics Paraffin Embedding Proteins Research Research Personnel Resources Scientist Services Slide Source Staining method Stains Students System Techniques Tissues Training United States National Institutes of Health Universities cellular imaging charge coupled device camera experience finesse fluorescence imaging human tissue immunocytochemistry meetings professor programs tissue culture tissue fixing tissue preparation tool

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项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The purpose of The MHFI core is to provide researchers with the resources to prepare tissue sections for histological and immunohistochemical staining. Toward this end the facility maintains the following equipment: + 2 microtomes for cutting paraffin embedded tissue- RMC MT 920 and Shandon Finesse 325 + 1 Shandon Citadel 2000 tissue processor + 1 Shandon Histocentre 2 embedding center + 1 Shandon Veristain 24-4 automated stainer for standard histology stains + 2 Cryotome cryostatic microtomes for frozen tissues one for human tissue, one for non human tissue + 1 vibratome for sectioning fixed tissues The core also maintains an available inventory of slides, coverslips, mounting media, waterbaths, and a variety of other histology tools. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. The MHFI provides researchers with analyzable images of cells/tissues. The core contains both the equipment and the technical assistance for acquiring and analyzing images taken from both the light and confocal microscope. The facility maintains the following equipment: + Olympus SZX16 Research Stereo Microscope, with a DP71- 12.5MP CCD Camera + 1 Nikon E800 research epifluorescent/transmitted light microscope with DIC optics + 1 Biorad Radiance 2000 confocal microscope on a Nikon TE300 inverted epifluor/ transmitted light microscope + 1 Nuance multi-spectral Image analysis system + NIH Imaging, Image J, CRi/Nuance Multispectral imaging, Image Pro Version 6.2 and Photoshop software + 3 Macintosh computers + 2 PC workstations The MHFI Core is under the direction of Dr. Diana Lurie (Professor, BMED). Dr. Lurie has 20 years of experience in both imaging and tissue preparation techniques including immunocytochemistry. She is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms. Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Diane Brooks, B.S., provides additional technical support in the core. Dr. Lurie, Ms. Brooks and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core.

该副本是利用众多研究子项目之一由NIH/NCRR资助的中心赠款提供的资源。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是对于中心，这不一定是调查员的机构。在组织或培养细胞中可视化和定位细胞蛋白的能力是任何生物学程序的重要组成部分。分子组织学和荧光成像核心致力于协助该系，蒙大拿州大学以及整个州内的研究人员，涉及蛋白质在组织培养以及组织切片中的鉴定，可视化和定位的项目。 MHFI核心的目的是为研究人员提供为组织学和免疫组织化学染色准备组织切片的资源。为此，该设施维护以下设备： + 2个用于切割石蜡嵌入组织的微失调-RMC MT 920和Shandon Finesse 325 + 1 Shandon Citadel 2000组织处理器 + 1 Shandon Histocentre 2嵌入中心 + 1 Shandon Verisain 24-4自动污渍用于标准组织学污渍 + 2个冷冻组的冷冻固定微动物，用于人体组织的一个冷冻组织，一种用于非人体组织 + 1个用于切片固定组织的振动型该核心还保持了幻灯片，盖玻片，安装媒体，水浴和各种其他组织学工具的可用清单。该核心雇用了一名专门从组织学和免疫组织化学（IHC）技术的员工科学家。科学家与研究人员进行实验设计协商，维护核心设备，为研究人员和学生以及其他相关服务提供组织学服务和培训。 MHFI为研究人员提供了可分析的细胞/组织图像。该核心包含从光和共聚焦显微镜中获取和分析图像的设备和技术援助。该设施维护以下设备： + Olympus SZX16研究立体声显微镜，带有DP71- 12.5MP CCD摄像头 + 1 Nikon E800研究表荧光度/发射光学显微镜 DIC光学元件 + 1 Biorad Radiance 2000尼康TE300上的共聚焦显微镜倒置 Epifluor/透射光学显微镜 + 1个差异多光谱图像分析系统 + NIH成像，图像J，CRI/NUANCE多光谱成像，图像Pro版本 6.2和Photoshop软件 + 3 Macintosh计算机 + 2个PC工作站 MHFI核心处于Diana Lurie博士的指导下（教授， BMed）。 Lurie博士在成像和组织制备技术（包括免疫细胞化学）方面具有20年的经验。她是MHFI Core的参谋科学家Lou Herritt的直接主管。赫里特女士是一位经验丰富的组织学家，对成像进行了广泛的培训，包括共聚焦显微镜技术的高级课程。 B.S.黛安·布鲁克斯（Diane Brooks）在核心中提供了其他技术支持。 Lurie博士，Brooks女士和Herritt女士每周见面，讨论核心设施的维护以及目前正在核心正在进行的科学项目。