IMAGING AND HISTOLOGY CORE

成像和组织学核心

• 批准号: 8167594
• 负责人: SCOTT Allen WETZEL
• 金额: $ 10.07万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167594
• 关键词: Biological; Computer Retrieval of Information on Scientific Projects Database; Computer software; Computers; Consult; Core Facility; Cultured Cells; Equipment; Equipment and supply inventories; Experimental Designs; Freezing; Funding; Grant; Histology; Image; Image Analysis; Imagery; Immunohistochemistry; Institution; Light; Light Microscope; Maintenance; Microscope; Microtome - medical device; Molecular; Montana; Optics; Paraffin Embedding; Proteins; Research; Research Personnel; Resources; Scientist; Services; Slide; Source; Staining method; Stains; Students; System; Techniques; Tissues; Training; United States National Institutes of Health; Universities; cellular imaging; charge coupled device camera; experience; finesse; fluorescence imaging; human tissue; immunocytochemistry; meetings; professor; programs; tissue culture; tissue fixing; tissue preparation; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The ability to visualize and localize cellular proteins in tissues or cultured cells is an essential component of any biological program. The Molecular Histology and Fluorescence Imaging Core is dedicated to assisting researchers within the department, the University of Montana, and across the state with projects involving the identification, visualization, and localization of proteins in tissue culture as well as in tissue sections. The purpose of The MHFI core is to provide researchers with the resources to prepare tissue sections for histological and immunohistochemical staining. Toward this end the facility maintains the following equipment: + 2 microtomes for cutting paraffin embedded tissue- RMC MT 920 and Shandon Finesse 325 + 1 Shandon Citadel 2000 tissue processor + 1 Shandon Histocentre 2 embedding center + 1 Shandon Veristain 24-4 automated stainer for standard histology stains + 2 Cryotome cryostatic microtomes for frozen tissues  one for human tissue, one for non  human tissue + 1 vibratome for sectioning fixed tissues The core also maintains an available inventory of slides, coverslips, mounting media, waterbaths, and a variety of other histology tools. The core employs a staff scientist versed in histology and immunohistochemistry (IHC) techniques. The scientist consults with researchers on experimental design, maintains the core equipment, provides histology services and training to researchers and students, and other related services as needed. The MHFI provides researchers with analyzable images of cells/tissues. The core contains both the equipment and the technical assistance for acquiring and analyzing images taken from both the light and confocal microscope. The facility maintains the following equipment: + Olympus SZX16 Research Stereo Microscope, with a DP71- 12.5MP CCD Camera + 1 Nikon E800 research epifluorescent/transmitted light microscope with DIC optics + 1 Biorad Radiance 2000 confocal microscope on a Nikon TE300 inverted epifluor/ transmitted light microscope + 1 Nuance multi-spectral Image analysis system + NIH Imaging, Image J, CRi/Nuance Multispectral imaging, Image Pro Version 6.2 and Photoshop software + 3 Macintosh computers + 2 PC workstations The MHFI Core is under the direction of Dr. Diana Lurie (Professor, BMED). Dr. Lurie has 20 years of experience in both imaging and tissue preparation techniques including immunocytochemistry. She is the direct supervisor of Lou Herritt, the Staff Scientist for the MHFI core. Ms. Herritt is an experienced histologist with extensive training in imaging, including advanced coursework in confocal microscope techniques. Diane Brooks, B.S., provides additional technical support in the core. Dr. Lurie, Ms. Brooks and Ms. Herritt meet weekly to discuss the maintenance of the core facility as well as scientific projects currently ongoing in the core.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 可视化和定位组织或培养细胞中的细胞蛋白的能力是任何生物程序的重要组成部分。 分子组织学和荧光成像核心致力于协助该系、蒙大拿大学和全州的研究人员开展涉及组织培养物和组织切片中蛋白质的识别、可视化和定位的项目。 MHFI 核心的目的是为研究人员提供准备用于组织学和免疫组织化学染色的组织切片的资源。为此，该设施维护以下设备： + 2 台用于切割石蜡包埋组织的切片机 - RMC MT 920 和 Shandon Finesse 325 + 1 个 Shandon Citadel 2000 组织处理机 + 1 Shandon Histocentre 2 包埋中心 + 1 台 Shandon Veristin 24-4 自动染色机，用于标准组织学染色 + 2 台用于冷冻组织的 Cryotome 低温切片机，一台用于人体组织，一台用于非人体组织 + 1 个振动切片机，用于固定组织切片 该核心还保留了载玻片、盖玻片、封固剂、水浴和各种其他组织学工具的可用库存。核心团队聘请了一位精通组织学和免疫组织化学 (IHC) 技术的科学家。 科学家就实验设计向研究人员进行咨询，维护核心设备，为研究人员和学生提供组织学服务和培训，以及根据需要提供其他相关服务。 MHFI 为研究人员提供可分析的细胞/组织图像。核心包含用于获取和分析从光学显微镜和共焦显微镜拍摄的图像的设备和技术援助。该设施维护以下设备： + Olympus SZX16 研究立体显微镜，配有 DP71- 12.5MP CCD 相机 + 1 台 Nikon E800 研究型落射荧光/透射光显微镜 DIC 光学器件 + 1 个安装在 Nikon TE300 倒置上的 Biorad Radiance 2000 共焦显微镜 落射荧光/透射光显微镜 + 1 Nuance 多光谱图像分析系统 + NIH 成像、Image J、CRi/Nuance 多光谱成像、Image Pro 版本 6.2 与Photoshop软件 + 3 台 Macintosh 电脑 + 2 个 PC 工作站 MHFI 核心由 Diana Lurie 博士（教授， BMED）。 Lurie 博士在成像和组织制备技术（包括免疫细胞化学）方面拥有 20 年的经验。 她是 MHFI 核心研究员 Lou Herritt 的直接主管。 Herritt 女士是一位经验丰富的组织学家，接受过广泛的成像培训，包括共焦显微镜技术的高级课程。 Diane Brooks，B.S.，提供额外的核心技术支持。 卢里博士、布鲁克斯女士和赫里特女士每周会面讨论核心设施的维护以及目前在核心进行的科学项目。