Molecular Histology and Fluorescent Imaging

分子组织学和荧光成像

• 批准号: 9039998
• 负责人: SCOTT Allen WETZEL
• 金额: $ 12.51万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9039998
• 关键词: Biological; Biological Models; Biology; Cells; Cellular Infiltration; Centers of Research Excellence; Consultations; Data; Data Analyses; Detection; Disease; Emerging Technologies; Environmental Health; Equipment; Event; Experimental Designs; Exposure to; Extramural Activities; Funding; Goals; Histological Techniques; Histology; Human Biology; Image; Image Analysis; Imaging technology; Immunofluorescence Immunologic; Immunohistochemistry; Instruction; Life; Membrane Proteins; Microscope; Mission; Molecular; Monitor; Montana; Nucleic Acids; Outcome; Preparation; Process; Regulation; Research; Research Personnel; Research Support; Resource Sharing; Resources; Sampling; Services; Staining method; Stains; Technical Expertise; Techniques; Technology; Time; Tissue imaging; Tissues; Toxic Environmental Substances; Toxicant exposure; Training; United States National Institutes of Health; Universities; Update; Xenobiotics; base; cost; design; fluorescence imaging; histological image; imaging system; insight; instrumentation; interest; member; operation; programs; research study; success; tool

PROJECT SUMMARY (See instructions): The ability to visualize and determine the localization of molecules within cells and tissues is an essential component of modern biology. Imaging and histology are central to the research mission of the CEHS and its members. The rapid advances in imaging technology within the last decade have greatly advanced the field and provided for new and exciting lines of inquiry. As imaging technologies have become more sophisticated, new and updated equipment and the expertise to use them efficiently and to their full potential is required for investigators to remain competitive. However, the costs and time required to master these techniques are prohibitive for most investigators. The purpose of the Molecular Histology and Fluorescence Imaging (MHFI) Core is to provide CEHS investigators with the means and expertise to perform cutting-edge histology and imaging experiments using state-of-the-art equipment and technical expertise. The histology component provides users with automated sample preparation, increasing throughput while at the same time standardizing sample preparation and staining. This is essential to CEHS investigators as they assess the biological outcome of exposure to environmental toxicants and xenobiotics. The fluorescence imaging component maintains a new, state-of-the-art Olympus FV1000 confocal microscope with spectral detection, live cell, 3D, and TIRF capabilities. The MHFI Core also has a Nuance Multispectral Imaging system and several off-line image analysis workstations allowing users sophisticated tools necessary to analyze their data. Most importantly, in addition to the equipment the Core provides investigators with expert advise and technical expertise to design, implement, and critically analyze histology and fluorescent imaging experiments. The unique services provided by the MHFI Core and its staff is critically important to the competitiveness and success of CEHS investigators and for investigators across the University of Montana and the region in obtaining and maintaining extramural funding.

项目摘要（请参阅说明）：可视化和确定细胞和组织中分子定位的能力是现代生物学的重要组成部分。成像和组织学是CEHS及其成员的研究任务的核心。在过去十年中，成像技术的快速进步已大大提高了该领域，并提供了新的令人兴奋的询问线。随着成像技术变得越来越复杂，新的和更新的设备以及有效地使用它们的专业知识，并且要使研究人员保持竞争力是必需的。但是，掌握这些技术所需的成本和时间对于大多数研究人员来说都是艰难的。分子组织学和荧光成像（MHFI）核心的目的是为CEHS研究人员提供使用最先进的设备和技术专业知识进行尖端的组织学和成像实验的手段和专业知识。组织学成分为用户提供自动样本准备，同时标准化样品制备和染色，从而增加了吞吐量。这对CEHS研究人员评估了暴露于环境有毒物质和异种生物的生物学结果。荧光成像组件具有具有光谱检测，活细胞，3D和TIRF功能的新的，最先进的FV1000共聚焦显微镜。 MHFI核心还具有细微的多光谱成像系统，并且几个离线图像分析工作站允许用户复杂的工具来分析其数据。最重要的是，除了设备外，核心还为研究人员提供了专家建议和技术专业知识，以设计，实施和严格分析组织学和荧光成像实验。 MHFI Core及其员工提供的独特服务对于CEHS调查人员以及蒙大拿大学和该地区的调查人员的竞争力和成功至关重要，以获得和维持壁外资金。