Molecular Histology and Fluorescent Imaging

分子组织学和荧光成像

• 批准号: 9039998
• 负责人: SCOTT Allen WETZEL
• 金额: $ 12.51万
• 依托单位: UNIVERSITY OF MONTANA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9039998
• 关键词: Biological; Biological Models; Biology; Cells; Cellular Infiltration; Centers of Research Excellence; Consultations; Data; Data Analyses; Detection; Disease; Emerging Technologies; Environmental Health; Equipment; Event; Experimental Designs; Exposure to; Extramural Activities; Funding; Goals; Histological Techniques; Histology; Human Biology; Image; Image Analysis; Imaging technology; Immunofluorescence Immunologic; Immunohistochemistry; Instruction; Life; Membrane Proteins; Microscope; Mission; Molecular; Monitor; Montana; Nucleic Acids; Outcome; Preparation; Process; Regulation; Research; Research Personnel; Research Support; Resource Sharing; Resources; Sampling; Services; Staining method; Stains; Technical Expertise; Techniques; Technology; Time; Tissue imaging; Tissues; Toxic Environmental Substances; Toxicant exposure; Training; United States National Institutes of Health; Universities; Update; Xenobiotics; base; cost; design; fluorescence imaging; histological image; imaging system; insight; instrumentation; interest; member; operation; programs; research study; success; tool

PROJECT SUMMARY (See instructions): The ability to visualize and determine the localization of molecules within cells and tissues is an essential component of modern biology. Imaging and histology are central to the research mission of the CEHS and its members. The rapid advances in imaging technology within the last decade have greatly advanced the field and provided for new and exciting lines of inquiry. As imaging technologies have become more sophisticated, new and updated equipment and the expertise to use them efficiently and to their full potential is required for investigators to remain competitive. However, the costs and time required to master these techniques are prohibitive for most investigators. The purpose of the Molecular Histology and Fluorescence Imaging (MHFI) Core is to provide CEHS investigators with the means and expertise to perform cutting-edge histology and imaging experiments using state-of-the-art equipment and technical expertise. The histology component provides users with automated sample preparation, increasing throughput while at the same time standardizing sample preparation and staining. This is essential to CEHS investigators as they assess the biological outcome of exposure to environmental toxicants and xenobiotics. The fluorescence imaging component maintains a new, state-of-the-art Olympus FV1000 confocal microscope with spectral detection, live cell, 3D, and TIRF capabilities. The MHFI Core also has a Nuance Multispectral Imaging system and several off-line image analysis workstations allowing users sophisticated tools necessary to analyze their data. Most importantly, in addition to the equipment the Core provides investigators with expert advise and technical expertise to design, implement, and critically analyze histology and fluorescent imaging experiments. The unique services provided by the MHFI Core and its staff is critically important to the competitiveness and success of CEHS investigators and for investigators across the University of Montana and the region in obtaining and maintaining extramural funding.

项目摘要（参见说明）：可视化和确定细胞和组织内分子定位的能力是现代生物学的重要组成部分。成像和组织学是 CEHS 及其成员研究任务的核心。过去十年成像技术的快速进步极大地推进了该领域的发展，并提供了新的、令人兴奋的研究方向。随着成像技术变得越来越复杂，研究人员需要新的和更新的设备以及有效使用它们并充分发挥其潜力的专业知识来保持竞争力。然而，掌握这些技术所需的成本和时间对于大多数研究人员来说是令人望而却步的。分子组织学和荧光成像 (MHFI) 核心的目的是为 CEHS 研究人员提供使用最先进的设备和技术专业知识进行尖端组织学和成像实验的手段和专业知识。组织学组件为用户提供自动化样品制备，提高通量，同时标准化样品制备和染色。这对于 CEHS 研究人员评估接触环境毒物和异生物质的生物学结果至关重要。荧光成像组件保留了新型、最先进的奥林巴斯 FV1000 共焦显微镜，具有光谱检测、活细胞、3D 和 TIRF 功能。 MHFI Core 还具有 Nuance 多光谱成像系统和多个离线图像分析工作站，为用户提供分析数据所需的复杂工具。最重要的是，除了设备之外，Core 还为研究人员提供设计、实施和批判性分析组织学和荧光成像实验的专家建议和技术专长。 MHFI 核心及其工作人员提供的独特服务对于 CEHS 调查人员的竞争力和成功以及整个蒙大拿大学和该地区的调查人员获得和维持校外资金至关重要。