OCT IMAGING OF SUB-SCLERAL CHANNELS CREATED WITH FEMTOSECOND

使用飞秒创建的巩膜下通道 OCT 成像

• 批准号: 8169491
• 负责人: ZHONGPING CHEN
• 金额: $ 1.37万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169491
• 关键词: Affect; Animal Model; Animals; Blindness; Cadaver; Cell Death; Cicatrix; Computer Retrieval of Information on Scientific Projects Database; Doctor of Philosophy; Drainage procedure; Evaluation; Eye; Eyedrops; Funding; Fundus; General Anesthesia; Glaucoma; Grant; IACUC; Image; In Vitro; Institution; Laboratories; Lasers; Life; Liquid substance; Location; Longevity; Measurement; Michigan; Modification; Normal Range; Operative Surgical Procedures; Oryctolagus cuniculus; Procedures; Production; Property; Protocols documentation; Recovery; Research; Research Personnel; Resources; Shapes; Source; Techniques; Technology; Testing; Tissues; United States National Institutes of Health; Universities; Wound Healing; aqueous; in vitro Model; in vivo; pressure; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Femtosecond lasers can be delivered through transparent and translucent tissue to perform high precision surgical procedures without damage to the superficial or adjacent tissues. These unique properties of the femtosecond laser-tissue interactions provides considerable potential advantage over traditional laser treatments used for glaucoma. Glaucoma is one of the leading causes of blindness with over 2 million people affected in the US alone. The condition is characterized by increased pressure in the eye which causes gradual, permanent cell death in the fundus resulting in blindness. Treatment of glaucoma focuses on lowering the eye pressure by reducing the production of fluid in the eye that maintains the pressure (aqueous) or increasing the drainage of this fluid out of the eye. At present, the traditional treatment would include use of eye drops, laser, or surgery however, use of these treatments may be cumbersome and poses a lot of complications such as scar formation especially after surgery. We hypothesize that the application of the femtosecond laser may be used to treat glaucoma. Among several potential advantages over the traditional glaucoma treatments the most important is its ability to create drainage channels for aqueous outflow without collateral damage to the overlying or adjacent tissue. This will likely increase the longevity of the drainage channel that can maintain eye pressure within normal range. To test our hypothesis, we propose the evaluation of femtosecond laser technology for the creation of drainage channels in an in vivo animal model. An in-vitro model of the aqueous outflow was already created by the same group in another laboratory (University of Michigan) as an initial step to understand the effect of femtosecond laser created drainage channels on the aqueous outflow in cadaver eyes. The best shape and depth of the channel was obtained from this cadaver eye experiment. After the in-vitro study, it is necessary to establish the efficacy of the femtosecond laser technology for the creation of drainage channels in a live animal model. Additionally, the efficacy of the drainage channels for decreassing intraoccular pressure can also evaluated in vivo. Aims: 1. Demonstrate the efficacy of femtosecond laser glaucoma treatment and optimize the procedure using in vivo animal models and standard measurement techniques of the aqueous outflow 2. Investigate longevity and patency of femtosecond laser-created outflow channels by performing wound-healing studies using in vivo animal models Following general anesthesia at another location, each rabbit will be transported to BLI for OCT imaging of its eyes and then returned to the investigator's lab for recovery. All animal studies will be performed under and in accordance with the UCI IACUC approved animal protocol #2005-2567, and that a copy of that protocol, the IACUC approval and any approved modifications of the protocol will be provided to BLI. Tibor Juhasz, PhD (Ph: 949-824-8769) will be designated as the responsible party for oversight of the animal procedures at BLI.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 飞秒激光器可以通过透明和半透明的组织传递，以执行高精度的手术程序，而不会损坏表面或相邻组织。 飞秒激光 - 组织相互作用的这些独特性能比用于青光眼的传统激光治疗具有很大的潜在优势。青光眼是失明的主要原因之一，仅在美国就受到了200万人的影响。该病情的特征是眼睛中的压力增加，导致眼底逐渐，永久性细胞死亡，导致失明。青光眼治疗的重点是减少眼睛中液体的产生，从而保持压力（水性）或从眼睛中增加这种液体的排水。 目前，传统的治疗方法包括使用眼滴，激光或手术，但是，使用这些治疗方法可能很麻烦，并且会造成许多并发症，例如疤痕形成，尤其是在手术后。我们假设飞秒激光的应用可用于治疗青光眼。与传统青光眼治疗相对于传统青光眼治疗的几种潜在优势，最重要的是它可以创建排水通道以进行水性流出的能力，而不会对上覆或相邻组织的附带损害。这可能会增加引流通道的寿命，从而在正常范围内保持眼压。 为了检验我们的假设，我们建议对飞秒激光技术进行评估，以在体内动物模型中创建排水通道。同一组已经在另一个实验室（密歇根大学）中创建了一个水流流出的体外模型，这是了解飞秒激光器在尸体外流的水性流出的第一步。 该通道的最佳形状和深度是从该尸体眼睛实验中获得的。 经过维特罗研究后，有必要建立飞秒激光技术在现场动物模型中创建排水通道的功效。此外，排水通道降低的副周围压力的功效也可以在体内评估。 目标： 1。证明飞秒激光青光眼治疗的功效，并使用体内动物模型和水性流出的标准测量技术优化了程序 2。通过使用体内动物模型进行伤口愈合研究，研究飞秒激光创建的流出流出通道的寿命和通畅性 在另一个位置进行全身麻醉之后，每只兔子将被运送到BLI进行OCT的眼睛成像，然后返回调查员的实验室进行恢复。 所有动物研究将根据UCI IACUC批准的动物方案＃2005-2567进行，并将该方案的副本，IACUC批准以及该方案的任何批准修改提供给BLI。 Tibor Juhasz，PhD博士（ph：949-824-8769）将被指定为BLI上动物程序监督的负责方。