Cell Culture and Genetic Engineering Core

细胞培养和基因工程核心

• 批准号: 7688357
• 负责人: JAYANTA ROY-CHOWDHURY
• 金额: $ 16.8万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7688357
• 关键词: Adenoviruses; Animal Model; Animals; Baculoviruses; Cell Culture Techniques; Cell Line; Cell Separation; Culture Media; Cultured Cells; Equipment; Evaluation; Freezing; Gene Transfer; Genes; Genetic; Genetic Engineering; Hepatocyte; Hormonal; Hormones; Human; Instruction; Liver; Maintenance; Microscope; Microscopy; Mycoplasma; Recombinants; Research; Research Personnel; Resected; Services; Subfamily lentivirinae; Testing; Transplantation; Viral Vector; base; cell growth; cell type; digital; human embryonic stem cell

The Cell Culture and Genetic Engineering Core has two components: I. Cell culture component: Objectives of this component are to provide Liver Research Center Investigators with: (i) isolated liver cells of consistently high quality, (iii) well-characterized frozen stocks of cell lines, (iv) specialized culture media, hormones and supplements, and matrix components for optimal cell growth; and (iv) equipment and instructions for use in isolation and culturing of various cell types derived from the liver and II. Genetic Engineering component: (i) The objectives are to assist Liver Center Investigators in developing recombinant viral vectors, (based on oncoretroviruses, lentiviruses, adenoviruses and baculoviruses) for use in their specific research, and (ii) Maintain equipment for microscopy and assist in routine evaluation of cultured cells following genetic or other manipulations. Services to be added in 2008 include (i) culturing human embryonic stem cells and providing these to Liver Center Investigators for differentiation into liver cells; (ii) providing primary human liver cells isolated from resected liver segments and procured livers that are not used for transplantation; and (iii) assisting Investigators in genetically marking liver cells for identification after transplantation into animal models.

细胞培养和基因工程核心有两个组成部分：I。细胞培养成分： 该组成部分的目标是为肝脏研究中心的调查人员提供：（i）孤立的肝脏 始终高质量的细胞（iii）特征良好的细胞系冷冻库存，（iv）专业 培养基，激素和补充剂以及基质成分，以实现最佳细胞生长； （iv） 用于隔离和培养从肝脏衍生的各种细胞类型的设备和说明 和II。基因工程组成部分：（i）目标是协助肝脏中心调查人员 开发重组病毒载体（基于癌中病毒，慢病毒，腺病毒和 杆状病毒）用于其特定研究，（ii）维护显微镜的设备并协助 在遗传或其他操纵后对培养细胞的常规评估中。要添加的服务 2008包括（i）培养人类胚胎干细胞并将其提供给肝脏中心研究者 分化为肝细胞； （ii）提供从切除的肝脏分离的原代人肝细胞 段和未用于移植的肝脏； （iii）协助调查人员 在移植到动物模型中后，遗传标记肝细胞以鉴定。