Cell Culture and Genetic Engineering Core

细胞培养和基因工程核心

• 批准号: 8500666
• 负责人: JAYANTA ROY-CHOWDHURY
• 金额: $ 15.17万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8500666
• 关键词: Adenoviruses; Animal Model; Animals; Baculoviruses; Cell Culture Techniques; Cell Line; Cell Separation; Culture Media; Cultured Cells; Equipment; Evaluation; Freezing; Gene Transfer; Genetic; Genetic Engineering; Hepatocyte; Hormonal; Hormones; Human; Instruction; Liver; Maintenance; Microscope; Microscopy; Mycoplasma; Research; Research Personnel; Resected; Services; Subfamily lentivirinae; Testing; Therapeutic Studies; Transplantation; base; cell growth; cell type; digital; gene therapy; human embryonic stem cell; recombinant viral vector

PURPOSE: The Cell Culture and Genetic Engineering Core has two components: I. Cell culture component: Objectives of this component are to provide Liver Research Center Investigators with: (i) isolated liver cells of consistently high quality, (iii) well-characterized frozen stocks of cell lines, (iv) specialized culture media, hormones and supplements, and matrix components for optimal cell growth; and (iv) equipment and instructions for use in isolation and culturing of various cell types derived from the liver and II. Genetic Engineering component: (i) The objectives are to assist Liver Center Investigators in developing recombinant viral vectors, (based on oncoretroviruses, lentiviruses, adenoviruses and baculoviruses) for use in their specific research, and (ii) Maintain equipment for microscopy and assist in routine evaluation of cultured cells following genetic or other manipulations. Services to be added in 2008 include (i) culturing human embryonic stem cells and providing these to Liver Center Investigators for differentiation into liver cells; (ii) providing primary human liver cells isolated from resected liver segments and procured livers that are not used for transplantation; and (iii) assisting Investigators in genetically marking liver cells for identification after transplantation into animal models.

目的： 细胞培养和基因工程核心有两个组成部分： I. 细胞培养部分： 该部分的目标是为肝脏研究中心研究人员提供：(i) 始终保持高质量的分离肝细胞，(iii) 特征明确的细胞系冷冻储备，(iv) 专门的培养基、激素和补充剂以及基质成分为了最佳的细胞生长； (iv) 用于分离和培养源自肝脏的各种细胞类型的设备和说明。基因工程部分：(i) 目标是协助肝脏中心研究人员开发重组病毒载体（基于癌逆转录病毒、慢病毒、腺病毒和杆状病毒）用于其具体研究，以及 (ii) 维护显微镜设备并协助遗传或其他操作后对培养细胞的常规评估。待添加的服务 2008 年包括 (i) 培养人类胚胎干细胞并将其提供给肝脏中心研究人员以分化为肝细胞； (ii) 提供从切除的肝段和获得的不用于移植的肝脏中分离的原代人肝细胞； (iii) 协助研究人员对肝细胞进行基因标记，以便在移植到动物模型后进行鉴定。