Imaging and Computational Resources Core

成像和计算资源核心

• 批准号: 8006843
• 负责人: Richard E Waugh
• 金额: $ 20.38万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8006843
• 关键词: Adhesives; Appointment; Atomic Force Microscopy; Biomedical Engineering; Boston; Calcium; Cell Adhesion Molecules; Cell surface; Cells; Computer software; Confocal Microscopy; Data; Endothelial Cells; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Funding; Grant; Image; Image Analysis; Imaging technology; In Situ; Institutes; Ligands; Maintenance; Measurement; Methodology; Microscope; Microscopy; Process; Research; Resources; Services; Signal Transduction; Staging; Technology; Traction; Universities; chemokine; computer infrastructure; computing resources; density; digital; digital imaging; image processing; improved; neutrophil; professor; programs; research study; software development; tool

DESCRIPTION OF SERVICES Image acquisition, image processing, image analysis, and detecfion and interpretafion of fiuorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve exisfing capabilifies and to develop new processing methodologies is essential for the effective and fimely completion of the proposed studies. The core includes access to confocal microscopy, flow cytometry, atomic force microscopy and total internal refiectance fluorescence microscopy (TIRFM), as well as support for the maintenance of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addifion, in the current application we propose to expand core capabilifies to include support for computational resources. Two Co-lnvestigators have been added to the project, Micah Dembo from Boston University, will provide expertise in analyzing traction force microscopy experiments, and David Gee, an Assistant Professor at Rochester Institute of Technology who holds an adjunct appointment in Biomedical Engineering at the University of Rochester, who is expert in parallel compufing applications. Thus, capabilities for both high end parallel compufing resources and the ability to analyze traction force microscopy experiments represent significant addifions to services provided by the Core. Projects 1, 2, 3 and 5 are engaged in research directions that will benefit from these new resources, and Project 4, to a lesser extent, will benefit from the availability of these capabilities. We have made progress in the past grant period in moving to new hardware platforms, moving to a largely digital imaging acquisition format. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for improving capabilifies of this new hardware and facilitafing its roufine use in the proposed studies. We also recognize that the digital worid is constantly evolving, and confinued support to troubleshoot established technologies and maintain needed levels of imaging capability will be needed throughout the funded period of the program.

服务说明 图像采集、图像处理、图像分析以及荧光检测和解释 信号是所有项目中使用的基本工具。硬件和软件的可用性 完成这些任务，并提供技术支持来维护和改进现有能力 开发新的加工方法对于有效、及时地完成 拟议的研究。 核心包括共聚焦显微镜、流式细胞术、原子力显微镜和总 内反射荧光显微镜 (TIRFM)，以及对硬件维护的支持 用于视频图像处理和软件开发，以方便从其中提取信息 实验图像。此外，在当前的应用中，我们建议扩展核心功能，包括 对计算资源的支持。该项目已增加两名联合研究员 Micah Dembo 来自波士顿大学，将提供分析牵引力显微镜实验的专业知识，大卫 Gee，罗切斯特理工学院助理教授，在 罗彻斯特大学生物医学工程专业，并行计算应用专家。因此， 高端并行计算资源的能力和分析牵引力显微镜的能力 实验代表了对核心提供的服务的重大补充。项目 1、2、3 和 5 是 从事将从这些新资源中受益的研究方向，项目 4 在较小程度上将 受益于这些功能的可用性。我们在过去的拨款期间在转向 新的硬件平台，转向主要是数字成像采集格式。主要目标之一 下一个资助期间的核心将是提供技术支持和资源，以改善 使这种新硬件具有功能并促进其在拟议研究中的常规使用。我们也认识到 数字世界在不断发展，并持续支持对现有技术进行故障排除 并在整个项目资助期间保持所需的成像能力水平。