Imaging and Computational Resources Core

成像和计算资源核心

• 批准号: 8502295
• 负责人: Richard E Waugh
• 金额: $ 16.65万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8502295
• 关键词: Adhesives; Appointment; Atomic Force Microscopy; Biomedical Engineering; Blood Cells; Blood Proteins; Blood Vessels; Boston; Calcium; Cell Adhesion Molecules; Cell surface; Cells; Computer software; Confocal Microscopy; Data; Detection; Endothelial Cells; Flow Cytometry; Fluorescence; Fluorescence Microscopy; Funding; Grant; Image; Image Analysis; Imaging technology; In Situ; Institutes; Ligands; Maintenance; Measurement; Methodology; Microscope; Microscopy; Process; Research; Research Personnel; Resources; Services; Signal Transduction; Staging; Technology; Time; Traction; Universities; chemokine; computer infrastructure; computing resources; density; digital; digital imaging; image processing; improved; instrumentation; neutrophil; parallel computing; professor; programs; research study; software development; tool

DESCRIPTION OF SERVICES Image acquisition, image processing, image analysis, and detection and interpretation of fluorescence signals are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and timely completion of the proposed studies. The core includes access to confocal microscopy, flow cytometry, atomic force microscopy and total internal reflectance fluorescence microscopy (TIRFM), as well as support for the maintenance of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. In addition, in the current application we propose to expand core capabilities to include support for computational resources. Two Co-investigators have been added to the project, Micah Dembo from Boston University, will provide expertise in analyzing traction force microscopy experiments, and David Gee, an Assistant Professor at Rochester Institute of Technology who holds an adjunct appointment in Biomedical Engineering at the University of Rochester, who is expert in parallel computing applications. Thus, capabilities for both high end parallel computing resources and the ability to analyze traction force microscopy experiments represent significant additions to services provided by the Core. Projects 1, 2, 3 and 5 are engaged in research directions that will benefit from these new resources, and Project 4, to a lesser extent, will benefit from the availability of these capabilities. We have made progress in the past grant period in moving to new hardware platforms, moving to a largely digital imaging acquisition format. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for improving capabilities of this new hardware and facilitating its routine use in the proposed studies. We also recognize that the digital world is constantly evolving, and continued support to troubleshoot established technologies and maintain needed levels of imaging capability will be needed throughout the funded period of the program.

服务说明 图像采集、图像处理、图像分析以及荧光信号的检测和解释是所有项目中使用的基本工具。完成这些任务的硬件和软件的可用性，以及维持和提高现有能力以及开发新的处理方法的技术支持的可用性对于有效和及时完成拟议的研究至关重要。 核心包括使用共焦显微镜、流式细胞术、原子力显微镜和全内反射荧光显微镜 (TIRFM)，以及对用于视频图像处理的硬件维护和软件开发以促进信息提取的支持来自实验图像。此外，在当前的应用程序中，我们建议扩展核心功能以包括对计算资源的支持。该项目增加了两名联合研究员，波士顿大学的 Micah Dembo 将提供分析牵引力显微镜实验的专业知识，罗彻斯特理工学院的助理教授 David Gee 在该大学生物医学工程领域担任兼职教授罗切斯特博士是并行计算应用方面的专家。因此，高端并行计算资源的能力和分析牵引力显微镜实验的能力代表了核心提供的服务的显着增加。项目 1、2、3 和 5 所从事的研究方向将从这些新资源中受益，而项目 4 将在较小程度上受益于这些能力的可用性。在过去的资助期内，我们在转向新的硬件平台、转向主要是数字图像采集格式方面取得了进展。核心在下一个资助期间的主要目标之一将是提供技术支持和资源，以提高这种新硬件的能力并促进其在拟议研究中的常规使用。我们还认识到，数字世界在不断发展，在整个项目资助期间，需要持续提供支持来排除现有技术的故障并保持所需的成像能力水平。