CORE--IMAGING

核心--成像

• 批准号: 6608260
• 负责人: Richard E Waugh
• 金额: $ 11万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6608260
• 关键词: bioimaging /biomedical imaging; biomedical facility; cell sorting; confocal scanning microscopy; electron microscopy; image processing; video microscopy; video recording system

Image acquisitions, image processing and image analysis are fundamental tools used in all of the projects. The availability of hardware and software to accomplish these tasks, and the availability of technical support to maintain and improve existing capabilities and to develop new processing methodologies is essential for the effective and time completion of the proposed studies. The core includes support for confocal microscopy, electron microscopy and cell sorting, as well as support for the purchase of hardware to be used for video image processing and the development of software to facilitate extraction of information from experimental images. Both projects 3 and 4 face substantial needs in the next two to three years for development of new image processing equipment and software. Current hardware, although still effective for the tasks for which it was designed, is no longer supported by the manufacturer, and so any component failure in a particular system will result in a substantial reduction in our capacity to process images. Given this situation it is clearly inadvisable to develop further protocols for the existing systems, particularly in light of advances in technology that have occurred in recent years. One of the primary objectives for the Core in the next funding period will be to provide the technical support and resources for acquiring new hardware, porting exiting capabilities to the new platform and developing new analysis routines to support recently developed experimental procedures. A new need within the program in the next period will be technical support for confocal imaging. The University will provide a confocal microscope to which the program will have adequate access, but technical support to operate the system will not be provided. The complexity of the system, combined with the special needs of the program (e.g. the need to image fluid-perfused microvascular systems in vivo and in vitro, and the need for micromanipulation and accompanying pressure control systems) make it imperative that the program have dedicated technical expertise to conduct these studies. This support will be provided by the core and be available to all three Rochester projects.

图像采集、图像处理和图像分析是基础 所有项目中使用的工具。硬件和可用性 完成这些任务的软件以及技术的可用性 支持维持和提高现有能力并开发新能力 处理方法对于有效和时间至关重要 完成拟议的研究。 核心包括对共焦显微镜、电子显微镜和 细胞分选，以及购买要使用的硬件的支持 为视频图像处理和软件开发提供便利 从实验图像中提取信息。项目3和项目4 未来两三年内面临巨大的发展需求 新的图像处理设备和软件。虽然目前的硬件 对于其设计的任务仍然有效，不再有效 由制造商支持，因此任何组件故障 特定系统将导致我们的容量大幅减少 来处理图像。鉴于这种情况，显然不建议 为现有系统开发进一步的协议，特别是在光方面 近年来发生的技术进步。中的一个 核心在下一个资助期内的主要目标是 提供获取新硬件的技术支持和资源， 将现有功能移植到新平台并开发新功能 分析例程支持最近开发的实验程序。 下一阶段该计划的新需求将是技术支持 用于共焦成像。该大学将提供共焦显微镜 该计划将有足够的访问权限，但技术支持 不提供操作系统。系统的复杂性， 结合程序的特殊需求（例如需要图像 体内和体外液体灌注微血管系统，以及需要 用于显微操作和随附的压力控制系统）使其 该计划必须拥有专门的技术专长来进行 这些研究。这种支持将由核心提供并可用 罗彻斯特的所有三个项目。