Cell Imaging

细胞成像

• 批准号: 7030417
• 负责人: JERROLD R. TURNER
• 金额: $ 12.16万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-21 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7030417
• 关键词: bioimaging /biomedical imaging; biomedical facility; cell line; cell sorting; charge coupled device camera; electrophysiology; fluorescence recovery after photobleaching; histology; immunofluorescence technique; immunoperoxidase; microscopy; molecular /cellular imaging; time resolved data; tissue /cell culture; tissue /cell preparation; transfection

The Cell and Tissue Imaging Core is designed to provide the investigators and projects within this Program Project access to state-of-the-art techniques and equipment and the expertise to use these to their fullest capabilities. Thus, in addition to provision of equipment, services offered will include technical support, training, and assistance with experimental design, data collection, data analysis, interpretation, and preparation of presentation quality images. The core is managed by Dr. Jerrold Turner, a practicing gastrointestinal surgical pathologist who also has extensive experience with generation of transfected cell lines, quantitative and qualitative fluorescent imaging approaches, and immunostaining techniques, and is located adjacent to Dr. Turner's office and laboratory space. An experienced dedicated technician will support the operation of the core and assist investigators with all aspects of core utilization. The Specific Aims of the Cell and Tissue Imaging Core are to provide the investigators and projects within this Program Project with 1. creation of cell lines expressing fluorescent fusion proteins, including construction of appropriate expression constructs and transfection, sorting, and maintenance of cell lines, 2. time lapse imaging of live cell monolayers with simultaneous electrophysiological measurement, 3. fluorescence recovery after photobleaching (FRAP) studies, 4. immunofluorescence of cultured cells and frozen tissue sections, 5. post acquisition processing, including quantitative analysis and deconvolution, 6. histological preparation and analysis of tissues from experimental animals, and 7. immunoperoxidase staining of animal tissues. The Cell and Tissue Imaging Core facilities include two epifluorescence microscopes with heated/cooled stages, z-motors, automated electronic filter wheels, and cooled CCD cameras. A separate two-headed light microscope with high resolution color digital camera is also available. Multiple offline data analysis workstations are available within the core. The Cell and Tissue Imaging Core is supported by other core facilities at The University of Chicago, including 2 confocal microscopes and 2 inverted epifluorescence microscopes within The Integrated Microscopy Core and high speed cell sorting within the Flow Cytometry Core. SUMMARY: This core provides both routine and sophisticated microscopy approaches to Program Project Investigators. These approaches require both equipment and technical skills that are not typically available to individual laboratories. Their availability to Program Project Investigators will make it possible to complete studies that could not otherwise be accomplished and will increase both efficiency and cost-effectiveness of other studies. Thus, this core is vital to the success of the overall Program Project.

细胞和组织成像核心旨在为该程序提供调查人员和项目 项目访问最先进的技术和设备以及将其充分利用的专业知识 功能。因此，除了提供设备外，提供的服务还将包括技术支持， 培训和实验设计，数据收集，数据分析，解释和协助 准备演示质量图像的准备。核心由杰罗尔德·特纳（Jerrold Turner）博士管理 胃肠道手术病理学家，他在转染的细胞中也有丰富的经验 线，定量和定性荧光成像方法以及免疫染色技术，IS 位于特纳博士的办公室和实验室空间附近。一位经验丰富的专门技术人员将 支持核心的操作，并协助调查人员核心利用的各个方面。具体 细胞和组织成像核心的目的是为该程序提供调查人员和项目 通过1。产生表达荧光融合蛋白的细胞系，包括构造 适当的表达构建和转染，分类和维护细胞系，2。时间流逝 具有同时电生理测量的活细胞单层成像，3。荧光 光漂白后的恢复（FRAP）研究，4。培养细胞的免疫荧光并冷冻 组织切片，5。采集后处理，包括定量分析和反卷积，6。 实验动物组织的组织学制备和分析，7。免疫过氧化物酶 动物组织的染色。细胞和组织成像核心设施包括两种落荧光 具有加热/冷却阶段，Z运动器，自动电子滤清器和冷却CCD的显微镜 相机。还提供了具有高分辨率彩色数码相机的单独的两头光显微镜。 核心内有多个离线数据分析工作站。细胞和组织成像核心是 在芝加哥大学的其他核心设施的支持下，包括2个共聚焦显微镜和2个 集成显微镜核心和高速细胞分选内的倒荧光显微镜 在流式细胞仪核心内。 摘要：此核心提供程序项目的常规和复杂的显微镜方法 调查人员。这些方法需要通常不可用的设备和技术技能 给个别实验室。他们对计划项目调查人员的可用性将使完成 否则无法完成的研究将提高效率和成本效益 其他研究。因此，该核心对于整个计划项目的成功至关重要。