Multipixel Hybrid Photon-Counting Detector for High-Throughput Single-Molecule As

用于高通量单分子作为的多像素混合光子计数探测器

基本信息

批准号：
7821468
负责人：
SHIMON WEISS
金额：
$ 22.31万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-05-05 至 2012-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The long-term objective of this project is to provide the biomedical research community with a new device for high-throughput single-molecule fluorescence assays. The domains of applications of these methods range from basic research to high-throughput diagnostics and drug screening. The goal of this research proposal is to develop an integrated system for multiplexed, high-throughput single-molecule assays. This system will be based on a new type of single-photon counting capable mutichannel hybrid photodetector (HPD) recently developed by Hamamatsu Photonics Corporation. We will incorporate this detector into a multiple excitation/multiple detection spot confocal microscope and demonstrate its capabilities in actual single-molecule assays. We expect this detector to find applications not only in fundamental research applications using single-molecule methods, but also in the emerging fields of high-throughput single-molecule diagnostics and drug screening. The specific aims of this research proposal are: (1) to develop an electronic interface and data acquisition system (including software) for the multichannel hybrid detector developed by Hamamatsu. (2) to integrate this system into a multiplexed single-molecule fluorescence excitation/detection confocal microscope. (3) to demonstrate the capabilities of this system for multiplexed, high-throughput single- molecule assays, by performing multiplexed fluorescence correlation spectroscopy (FCS) measurements on model systems of biological relevance as well as multiplexed single-molecule FRET measurements using an alternate laser excitation (ALEX) scheme.

描述（由申请人提供）：该项目的长期目标是为生物医学研究界提供一种用于高通量单分子荧光测定的新设备。这些方法的应用领域范围从基础研究到高通量诊断和药物筛查。该研究建议的目的是开发一个用于多路复用的高通量单分子测定的集成系统。该系统将基于一种新型的单光子计数类型的杂种杂种杂交光电探测器（HPD），该系统最近由Hamamatsu Photonics Corporation开发。我们将将该检测器纳入多个激发/多重检测点共聚焦显微镜中，并在实际的单分子测定中证明其功能。我们希望该探测器不仅在基本研究应用中使用单分子方法找到应用，而且还可以在高通量单分子诊断和药物筛查的新兴领域中找到应用。该研究建议的具体目的是：（1）开发由Hamamatsu开发的多通道混合检测器的电子界面和数据采集系统（包括软件）。（2）将该系统整合到多路复用的单分子荧光激发/检测共聚焦显微镜中。（3）通过使用替代激发（ALEX）的多重单分子FRET测量值（ALEX）方案，通过对生物学相关性系统以及多路复用的单分子FRET测量进行多重荧光相关光谱（FCS）测量来证明该系统对多路复用，高通量单分子测定的能力。