High-Resolution, High Speed, High-Throughput 3-Dimensional Detector for Biology

适用于生物学的高分辨率、高速、高通量 3 维探测器

• 批准号: 7197027
• 负责人: SHIMON WEISS
• 金额: $ 31.91万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7197027
• 关键词: 3-Dimensional; Animals; Anisotropy; Anodes; Arts; Behavior; Biochemical; Biological; Biology; Biophysics; Caliber; Cell physiology; Cells; Characteristics; Charge; Complex; Computer software; Count; Data; Data Analyses; Detection; Development; Devices; Diagnosis; Dimensions; Disease; Electronics; Elements; Event; Evolution; Fluorescence; Fluorescence Microscopy; Fluorescence Resonance Energy Transfer; Fluorescence Spectroscopy; Future; Generations; Height; Human; Image; Interdisciplinary Study; Lasers; Lead; Life; Measures; Medical; Medicine; Methodology; Microfluidics; Microscopic; Microscopy; Mission; Molecular; Molecular Probes; Monitor; NIH Program Announcements; National Institute of General Medical Sciences; Noise; Patients; Performance; Photons; Physics; Physiologic pulse; Positioning Attribute; Protocols documentation; Public Health; Pulse taking; Range; Rate; Research; Research Personnel; Research Proposals; Resolution; Scientist; Semiconductors; Signal Transduction; Spectrum Analysis; Speed; Surface; Techniques; Time; Tissues; Training; Tube; Vision; animal tissue; base; bioimaging; data acquisition; design; detector; digital; gallium arsenide; in vivo; instrument; instrumentation; macromolecule; multidisciplinary; nanocrystal; photomultiplier; programs; protein folding; prototype; quantum; single molecule; symposium

DESCRIPTION (provided by applicant): The objectives of this proposal are to develop a high-performance photon-counting 3-dimensional imager (2 spatial, 1 temporal) for biology and new methodologies and protocols taking advantage of its new capabilities. This detector will be optimized for ultrahigh sensitivity fluorescence spectroscopy in the visible and near-infrared spectral range (500-850 nm). It will have: (i) a high detection efficiency based on a fast GaAs photocathode: ~35 %, (ii) will register single-photons with high spatial resolution using a cross-strip anode design: 35 um, and (iii) high temporal resolution using microchannel plates: 250 ps, and (iv) be capable of both high local and global couting rates (100 kHz, 20 MHz). These characteristics are condensed in the acronym "H33D" (pronounced heed) for High-spatial, High-temporal resolution, and High throughput (the 3 "H'"s, hence H3) 3-Dimensional detector. The H33D detector will be suitable for a variety of biomedical applications, such as imaging and spectroscopy of single molecules, molecular complexes and macromolecules in living cells and tissues, as well as in vivo animal imaging, some of which will be studied during the proposed 4 year span of this research proposal. The last application will be a first step towards using the H33D detector for biomedical imaging of fluorescent molecular probes in human patients with unprecedented sensitivity. These characteristics of our interdisciplinary research proposal regrouping scientists with a biophysical/biochemical background and scientists with a physics/instrumentation background are in perfect match with the vision expressed during the Biomedical Imaging Symposium 'Visualizing the Future of Biology and Medicine" organized by the BECON in 1999, and fits particularly well within the mission of the NIGMS and the NIBIB, who are part of BRG program announcement PA-02-011. Relevance of this research to public health: The richer information provided by the H33D detector will lead to a better understanding of cellular processes at the molecular level, a prerequisite step to design intelligent counter-measures aimed at curing abnormal cellular behavior with medical compounds. The long-term application of the H33D detector for live animal and human imaging will provide a much needed high- sensitivity instrument for diagnosing and monitoring the evolution of diseases at the molecular level.

描述（由申请人提供）：该提案的目标是为生物学和新的方法和协议开发高性能光子计算3维成像仪（2个空间，1个时间段），利用其新功能。该检测器将在可见的和近红外光谱范围内（500-850 nm）中优化用于超高灵敏度荧光光谱。它将具有：（i）基于快速GAAS光电阴极的高检测效率：〜35％，（ii）将使用跨条纹阳极设计进行高空间分辨率的单光子注册：35 um和（iii）使用微频道板的高时间分辨率使用微渠道板：250 ps和（iv）具有高度和（iiv）的高度和全局速率（IV）。这些特征以高空间，高速度分辨率和高吞吐量（3“ h'” S，因此H3）3维检测器的首字母缩写为“ H33D”（发音为注意）。 H33D检测器将适用于各种生物医学应用，例如活细胞和组织中的单分子，分子复合物和大分子的成像和光谱，以及体内动物成像，其中一些将在提出的4年研究范围内研究。最后的应用将是使用H33D检测器对具有前所未有敏感性的人类患者的荧光分子探针进行生物医学成像的第一步。我们的跨学科研究建议的这些特征，使科学家具有生物物理/生化背景和具有物理/仪器背景的科学家，这与在生物医学成像研讨会“可视化生物学和医学的未来”中表达的愿景完全匹配。 PA-02-011。分子水平疾病的进化。