High-Resolution, High Speed, High-Throughput 3-Dimensional Detector for Biology

适用于生物学的高分辨率、高速、高通量 3 维探测器

基本信息

批准号：
7393254
负责人：
SHIMON WEISS
金额：
$ 35.99万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2011-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7393254
关键词：
3-Dimensional Animals Anisotropy Anodes Arts Behavior Biochemical Biological Biology Biophysics Caliber Cell physiology Cells Characteristics Charge Complex Computer software Count Data Data Analyses Detection Development Devices Diagnosis Dimensions Disease Electronics Elements Event Evolution Fluorescence Fluorescence Microscopy Fluorescence Resonance Energy Transfer Fluorescence Spectroscopy Future Generations Height Human Image Interdisciplinary Study Lasers Lead Life Measures Medical Medicine Methodology Microfluidics Microscopic Microscopy Mission Molecular Molecular Probes Monitor NIH Program Announcements National Institute of General Medical Sciences Noise Patients Performance Photons Physics Physiologic pulse Positioning Attribute Protocols documentation Public Health Pulse taking Range Rate Research Research Personnel Research Proposals Resolution Scientist Semiconductors Signal Transduction Spectrum Analysis Speed Surface Techniques Time Tissues Training Tube Vision animal tissue base bioimaging data acquisition design detector digital gallium arsenide in vivo instrument instrumentation macromolecule multidisciplinary nanocrystal photomultiplier programs protein folding prototype quantum single molecule symposium

项目摘要

DESCRIPTION (provided by applicant): The objectives of this proposal are to develop a high-performance photon-counting 3-dimensional imager (2 spatial, 1 temporal) for biology and new methodologies and protocols taking advantage of its new capabilities. This detector will be optimized for ultrahigh sensitivity fluorescence spectroscopy in the visible and near-infrared spectral range (500-850 nm). It will have: (i) a high detection efficiency based on a fast GaAs photocathode: ~35 %, (ii) will register single-photons with high spatial resolution using a cross-strip anode design: 35 um, and (iii) high temporal resolution using microchannel plates: 250 ps, and (iv) be capable of both high local and global couting rates (100 kHz, 20 MHz). These characteristics are condensed in the acronym "H33D" (pronounced heed) for High-spatial, High-temporal resolution, and High throughput (the 3 "H'"s, hence H3) 3-Dimensional detector. The H33D detector will be suitable for a variety of biomedical applications, such as imaging and spectroscopy of single molecules, molecular complexes and macromolecules in living cells and tissues, as well as in vivo animal imaging, some of which will be studied during the proposed 4 year span of this research proposal. The last application will be a first step towards using the H33D detector for biomedical imaging of fluorescent molecular probes in human patients with unprecedented sensitivity. These characteristics of our interdisciplinary research proposal regrouping scientists with a biophysical/biochemical background and scientists with a physics/instrumentation background are in perfect match with the vision expressed during the Biomedical Imaging Symposium 'Visualizing the Future of Biology and Medicine" organized by the BECON in 1999, and fits particularly well within the mission of the NIGMS and the NIBIB, who are part of BRG program announcement PA-02-011. Relevance of this research to public health: The richer information provided by the H33D detector will lead to a better understanding of cellular processes at the molecular level, a prerequisite step to design intelligent counter-measures aimed at curing abnormal cellular behavior with medical compounds. The long-term application of the H33D detector for live animal and human imaging will provide a much needed high- sensitivity instrument for diagnosing and monitoring the evolution of diseases at the molecular level.

描述（由申请人提供）：本提案的目标是开发一种用于生物学的高性能光子计数 3 维成像仪（2 个空间，1 个时间），以及利用其新功能的新方法和协议。该探测器将针对可见光和近红外光谱范围（500-850 nm）的超高灵敏度荧光光谱进行优化。它将具有：(i) 基于快速 GaAs 光电阴极的高检测效率：约 35%，(ii) 将使用交叉带阳极设计以高空间分辨率记录单光子：35 um，以及 (iii) 高使用微通道板的时间分辨率：250 ps，并且 (iv) 能够实现高局部和全局计算速率（100 kHz、20 MHz）。这些特性浓缩在缩写“H33D”（发音为 heed）中，代表高空间、高时间分辨率和高吞吐量（3 个“H”，因此为 H3）3 维探测器。 H33D探测器将适用于各种生物医学应用，例如活细胞和组织中的单分子、分子复合物和大分子的成像和光谱，以及体内动物成像，其中一些将在拟议的4期间进行研究本研究计划的跨度为一年。最后的应用将是使用 H33D 探测器以前所未有的灵敏度对人类患者进行荧光分子探针生物医学成像的第一步。我们的跨学科研究提案将具有生物物理/生化背景的科学家和具有物理/仪器背景的科学家重新组合，这些特点与 BECON 在1999 年，特别适合 NIGMS 和 NIBIB 的使命，它们是 BRG 计划公告 PA-02-011 的一部分。这项研究与公众的相关性。健康：H33D探测器提供的更丰富的信息将有助于在分子水平上更好地了解细胞过程，这是设计旨在用医学化合物治愈异常细胞行为的智能对策的先决条件。用于活体动物和人体成像的 H33D 探测器将为在分子水平上诊断和监测疾病的演变提供急需的高灵敏度仪器。