Multipixel Hybrid Photon-Counting Detector for High-Throughput Single-Molecule As

用于高通量单分子作为的多像素混合光子计数探测器

• 批准号: 7446034
• 负责人: SHIMON WEISS
• 金额: $ 22.09万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-05 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7446034
• 关键词: Basic Science; Biological; Biological Assay; Biological Models; Biomedical Research; Communities; Computer software; Count; Detection; Devices; Diagnostic; Electronics; Fluorescence; Goals; Hybrids; Lasers; Measurement; Methods; Microscope; Photons; Preclinical Drug Evaluation; Range; Research Proposals; Scheme; Spectrum Analysis; Spottings; System; base; data acquisition; detector; fundamental research; interest; photonics; single molecule; single-molecule FRET

DESCRIPTION (provided by applicant): The long-term objective of this project is to provide the biomedical research community with a new device for high-throughput single-molecule fluorescence assays. The domains of applications of these methods range from basic research to high-throughput diagnostics and drug screening. The goal of this research proposal is to develop an integrated system for multiplexed, high-throughput single-molecule assays. This system will be based on a new type of single-photon counting capable mutichannel hybrid photodetector (HPD) recently developed by Hamamatsu Photonics Corporation. We will incorporate this detector into a multiple excitation/multiple detection spot confocal microscope and demonstrate its capabilities in actual single-molecule assays. We expect this detector to find applications not only in fundamental research applications using single-molecule methods, but also in the emerging fields of high-throughput single-molecule diagnostics and drug screening. The specific aims of this research proposal are: (1) to develop an electronic interface and data acquisition system (including software) for the multichannel hybrid detector developed by Hamamatsu. (2) to integrate this system into a multiplexed single-molecule fluorescence excitation/detection confocal microscope. (3) to demonstrate the capabilities of this system for multiplexed, high-throughput single- molecule assays, by performing multiplexed fluorescence correlation spectroscopy (FCS) measurements on model systems of biological relevance as well as multiplexed single-molecule FRET measurements using an alternate laser excitation (ALEX) scheme.

描述（由申请人提供）：该项目的长期目标是为生物医学研究界提供一种用于高通量单分子荧光测定的新设备。这些方法的应用领域从基础研究到高通量诊断和药物筛选。该研究计划的目标是开发一种用于多重、高通量单分子检测的集成系统。该系统将基于滨松光子公司最近开发的一种新型单光子计数多通道混合光电探测器（HPD）。我们将将该检测器整合到多激发/多检测点共焦显微镜中，并在实际的单分子测定中展示其功能。我们期望该检测器不仅能够在使用单分子方法的基础研究应用中找到应用，而且能够在高通量单分子诊断和药物筛选的新兴领域中找到应用。本研究计划的具体目标是：（1）为滨松开发的多通道混合探测器开发电子接口和数据采集系统（包括软件）。 (2)将该系统集成到多重单分子荧光激发/检测共焦显微镜中。 (3) 通过对生物相关模型系统进行多重荧光相关光谱 (FCS) 测量以及使用备用激光器进行多重单分子 FRET 测量，展示该系统进行多重、高通量单分子测定的能力激励（ALEX）方案。