Role of GDF11 in the developing and adult pancreas

GDF11 在发育中和成人胰腺中的作用

• 批准号: 7734238
• 负责人: Alexandra Christine McPherron
• 金额: $ 13.92万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7734238
• 关键词: Address; Adult; Age; Birth; Body Composition; Body Weights and Measures; Cells; Cultured Cells; Defect; Development; Diabetes Mellitus; Diet; Differentiation and Growth; Fatty acid glycerol esters; Gene Expression; Gene Targeting; Gene Transfer Techniques; Genes; Goals; Growth; Kidney; Knockout Mice; Methods; Mus; Mutant Strains Mice; Pancreas; Perinatal; Phenotype; Role; Signal Transduction; Skeletal system; Tissues; Transforming Growth Factor beta; glucose metabolism; islet; member; mouse Gdf11 protein; postnatal; sex

In order to overcome the perinatal lethality of GDF11 deletion in mice, we have made a pancreas specific deletion of the mouse GDF11 gene using the Cre-loxP method. We have characterized the phenotype by measuring body weight during postnatal growth and body composition in both sexes. We have also analyzed glucose metabolism in adult mice of different ages and on high fat diets. We have determined gene expression differences in islets from wild type and mutant mice at several ages and both sexes and are analyzing the cell subtype proportions.

为了克服小鼠 GDF11 缺失的围产期致死率，我们使用 Cre-loxP 方法对小鼠 GDF11 基因进行了胰腺特异性缺失。我们通过测量两性出生后生长期间的体重和身体成分来表征该表型。我们还分析了不同年龄和高脂肪饮食的成年小鼠的葡萄糖代谢。我们已经确定了野生型和突变型小鼠在不同年龄和性别的胰岛中的基因表达差异，并正在分析细胞亚型比例。