Regulation of Skeletal Muscle Mass

骨骼肌质量的调节

• 批准号: 7734344
• 负责人: Alexandra Christine McPherron
• 金额: $ 27.85万
• 依托单位: NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7734344
• 关键词: Adult; Cachexia; Cell Culture Techniques; Child; Development; Disease; Fiber; Frequencies; HIV Wasting Syndrome; In Vitro; Knockout Mice; Limb structure; Malignant Neoplasms; Molecular; Mus; Muscle; Muscle Development; Muscle Fibers; Muscle satellite cell; Muscular Dystrophies; Mutant Strains Mice; Numbers; Primary Cell Cultures; Proteins; Regulation; Role; Siblings; Signal Transduction; Skeletal Muscle; Skeletal system; Staging; Stem cells; Transgenic Mice; embryonic stem cell; in vivo; myostatin; satellite cell; size

For an in vivo assessment of skeletal muscle development in myostatin deficient mice, we have collected mouse limb muscle from many stages of development and early adulthood. We have determined the skeletal muscle precursor frequency and muscle fiber number in normal and myostatin knockout mice. We are making genetically modified mice to manipulate myostatin signaling in specific cell progenitors. We are also analyzing the role of myostatin in function of muscle stem cells in vitro and in vivo.

为了对肌生长抑制素缺陷小鼠的骨骼肌发育进行体内评估，我们收集了多个发育阶段和成年早期的小鼠肢体肌肉。我们测定了正常小鼠和肌生长抑制素敲除小鼠的骨骼肌前体频率和肌纤维数量。我们正在培育转基因小鼠来操纵特定细胞祖细胞中的肌生长抑制素信号传导。我们还在体外和体内分析肌生长抑制素在肌肉干细胞功能中的作用。