HOMOZYGOUS CLONES OF TROUT AS MODELS

鳟鱼的纯合克隆作为模型

• 批准号: 3421680
• 负责人: GARY H THORGAARD
• 金额: $ 14.76万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1995-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3421680
• 关键词: animal population genetics; biological models; chemical fingerprinting; environmental toxicology; genetic models; genetic strain; health science research; homozygote; immunogenetics; inbreeding; model design /development; molecular cloning; restriction fragment length polymorphism; trout /salmon; water pollution control

The purpose of the present proposal is to develop and describe syngeneic (isogenic) strains of rainbow trout as a lower vertebrate aquatic animal model for biomedical research. In particular, we will develop these fish for the study of immunological phenomena. These trout will be useful not only for immunological research such as immunogenetics and neuroimmunology, but will fill many niches in such diverse areas as endocrinology, developmental biology, aquatic toxicology, gene transfer technology and carcinogenesis research. The specific aims of the project are the propagation of existing and development of new isogenic lines by androgenesis and gynogenesis, validation of the isogenic nature of the derived fish; and further development of immunological techniques using the trout as models.

本提案的目的是开发和描述同基因 作为低等脊椎动物水生动物的虹鳟鱼（等基因）品系 生物医学研究模型。 特别是，我们将开发这些鱼 用于免疫学现象的研究。 这些鳟鱼会有用 仅用于免疫遗传学和神经免疫学等免疫学研究， 但将填补内分泌学等不同领域的许多空白， 发育生物学、水生毒理学、基因转移技术和 致癌作用研究。 该项目的具体目标是 现有的繁殖和新等基因系的开发 雄激素发生和雌激素发生，验证同基因性质 衍生鱼；以及利用免疫学技术的进一步发展 鳟鱼作为模型。