HOMOZYGOUS CLONES OF TROUT AS BIOMEDICAL RESEARCH MODELS

鳟鱼纯合克隆作为生物医学研究模型

基本信息

批准号：
6044120
负责人：
GARY H THORGAARD
金额：
$ 22.41万
依托单位：
WASHINGTON STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1990
资助国家：
美国
起止时间：
1990-09-30 至 2001-07-31
项目状态：
已结题

项目摘要

DESCRIPTION (Adapted from the applicant's abstract): The overall objective of this application is to continue the development and characterization of clonal lines of rainbow trout (Oncorhynchus mykiss) as models for biomedical research. Tasks of the present project will be divided between two laboratories: Dr. Gary Thorgaard's laboratory in the Department of Zoology, WSU, and Dr. Sandra Ristow's Laboratory in the Department of Animal Sciences, WSU. Lines of trout will be propagated and developmentally and behaviorally characterized by Dr. Gary Thorgaard's laboratory, and they will be immunologically characterized by Dr. Sandra Ristow's laboratory. The Specific Aims of the joint project are: 1) propagate and disseminate these trout as biomedical models; 2) examine developmental characteristics (development rate at three different temperatures, several meristic characters, and three measures of smoltification) in five clonal lines of rainbow trout; 3) examine behavioral and stress responses in five clonal lines of rainbow trout; 4) more fully characterize the T-cell like compartment and the natural cytotoxic cell activity of the lines of trout by completing the establishment of syngeneic cell lines, more fully characterize mixed lymphocyte cultures between the lines, graft across allogeneic lines, and examine natural cytotoxic cell (NCC) activity in the anterior kidneys of the trout; and 5) clone and sequence the following molecules that are trout analogues of the important T-cell function molecules of murine immune system: the major histocompatibility I molecule (MHCI), the beta chain of the putative T-cell receptor, and beta 2 microglobulin in the five clonal lines of trout.

描述（改编自申请人的摘要）：总体目标该应用程序的目的是继续开发和表征虹鳟鱼（Oncorhynchus mykiss）克隆系作为生物医学模型研究。本项目的任务将分为两部分实验室：动物学系 Gary Thorgaard 博士的实验室， WSU 和 Sandra Ristow 博士动物系实验室科学，华盛顿州立大学。鳟鱼品系将得到繁殖和发育 Gary Thorgaard 博士实验室的行为特征，他们会由 Sandra Ristow 博士的实验室进行免疫学表征。这该联合项目的具体目标是： 1) 宣传和传播这些鳟鱼作为生物医学模型； 2）检查发育特征（三种不同温度下的发育速率，几种分光度特征和银化的三种测量）在五个克隆系中虹鳟鱼； 3) 检查五个克隆的行为和压力反应虹鳟鱼线； 4）更全面地表征T细胞，例如隔室和鳟鱼品系的天然细胞毒性细胞活性更全面地完成同系细胞系的建立表征线间混合淋巴细胞培养物、移植物同种异体细胞系，并检查自然细胞毒性细胞 (NCC) 活性鳟鱼的前肾； 5) 克隆并测序以下内容重要 T 细胞功能的鳟鱼类似物分子鼠免疫系统分子：主要组织相容性 I 分子 (MHCI)、推定 T 细胞受体的 β 链和 β 2 鳟鱼的五个克隆系中的微球蛋白。