HOMOZYGOUS CLONES OF TROUT AS BIOMEDICAL RESEARCH MODELS

鳟鱼纯合克隆作为生物医学研究模型

• 批准号: 2751023
• 负责人: GARY H THORGAARD
• 金额: $ 21.76万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2751023
• 关键词: MHC class I antigen; animal extract; animal population genetics; behavior test; biological models; cellular immunity; cytotoxic T lymphocyte; ethology; genetic models; growth /development; homologous transplantation; homozygote; immunogenetics; mixed tissue /cell culture; model design /development; molecular cloning; morphology; nucleic acid sequence; physiologic stressor; quantitative trait loci; temperature; trout /salmon

DESCRIPTION (Adapted from the applicant's abstract): The overall objective of this application is to continue the development and characterization of clonal lines of rainbow trout (Oncorhynchus mykiss) as models for biomedical research. Tasks of the present project will be divided between two laboratories: Dr. Gary Thorgaard's laboratory in the Department of Zoology, WSU, and Dr. Sandra Ristow's Laboratory in the Department of Animal Sciences, WSU. Lines of trout will be propagated and developmentally and behaviorally characterized by Dr. Gary Thorgaard's laboratory, and they will be immunologically characterized by Dr. Sandra Ristow's laboratory. The Specific Aims of the joint project are: 1) propagate and disseminate these trout as biomedical models; 2) examine developmental characteristics (development rate at three different temperatures, several meristic characters, and three measures of smoltification) in five clonal lines of rainbow trout; 3) examine behavioral and stress responses in five clonal lines of rainbow trout; 4) more fully characterize the T-cell like compartment and the natural cytotoxic cell activity of the lines of trout by completing the establishment of syngeneic cell lines, more fully characterize mixed lymphocyte cultures between the lines, graft across allogeneic lines, and examine natural cytotoxic cell (NCC) activity in the anterior kidneys of the trout; and 5) clone and sequence the following molecules that are trout analogues of the important T-cell function molecules of murine immune system: the major histocompatibility I molecule (MHCI), the beta chain of the putative T-cell receptor, and beta 2 microglobulin in the five clonal lines of trout.

描述（改编自申请人的摘要）：总体目标 该应用程序的目的是继续开发和表征 虹鳟鱼（Oncorhynchus mykiss）克隆系作为生物医学模型 研究。 本项目的任务将分为两部分 实验室：动物学系 Gary Thorgaard 博士的实验室， WSU 和 Sandra Ristow 博士动物系实验室 科学，华盛顿州立大学。 鳟鱼品系将得到繁殖和发育 Gary Thorgaard 博士实验室的行为特征，他们会 由 Sandra Ristow 博士的实验室进行免疫学表征。 这 该联合项目的具体目标是： 1) 宣传和传播这些 鳟鱼作为生物医学模型； 2）检查发育特征 （三种不同温度下的发育速率，几种分光度 特征和银化的三种测量）在五个克隆系中 虹鳟鱼； 3) 检查五个克隆的行为和压力反应 虹鳟鱼线； 4）更全面地表征T细胞，例如 隔室和鳟鱼品系的天然细胞毒性细胞活性 更全面地完成同系细胞系的建立 表征线间混合淋巴细胞培养物、移植物 同种异体细胞系，并检查自然细胞毒性细胞 (NCC) 活性 鳟鱼的前肾； 5) 克隆并测序以下内容 重要 T 细胞功能的鳟鱼类似物分子 鼠免疫系统分子：主要组织相容性 I 分子 (MHCI)、推定 T 细胞受体的 β 链和 β 2 鳟鱼的五个克隆系中的微球蛋白。