University of Kansas Alzheimers Disease Center P30 Neuropathology supplement

堪萨斯大学阿尔茨海默病中心 P30 神经病理学补充

基本信息

批准号：
10741516
负责人：
RUSSELL H. SWERDLOW
金额：
$ 33.66万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-15 至 2026-06-30
项目状态：
未结题

项目摘要

PROJECT SUMMARY This Supplement requests one year of funding to support unforeseen costs associated with the University of Kansas Alzheimer’s Disease Research Center (KU ADRC) Neuropathology (NP) Core. The Neuropathology Core provides critical KU ADRC infrastructure in collaborating with other ADRC Cores (Figure 1). The neuropathology core function is essential to the Center’s mission and scientific theme by 1) Maintaining the autopsy program and Brain Bank; 2) Providing neuropathologic diagnosis, consultation, and training for investigators and trainees; 3) Obtaining dural and skin biopsies for induced pluripotent stem cell (IPSC) lines; and 4) Providing brain tissue for mitochondrial DNA (mtDNA) sequencing by the Biomarker Core. The NP Core continually upgrades it status to the diagnostic advances in the field and evolves according to the approved and accepted new guidelines, knowledge and state-of-the-art technology. Furthermore, the Core also participates in the important debates that produce these advances. An unanticipated growth in the number of investigators requesting NP Core support, an emergent unforeseen condition limiting our core to create a digital library of glass slides, increasing request for postmortem CSF specimens and an unpredicted obsolescence of our essential equipment to be replaced, predicates this request. We proposed in our recently funded P30 to provide open libraries of whole-slide scanned images to computer scientists and other investigators utilizing the scanners located in the department of pathology. However, due to increasing demand for neoplastic and other pathology specimens in the department, we have not been able to use this service properly as the current scanner is slow and the department cannot allocate proper time needed for our “research” projects. On the other hand, we have started working with computer scientists in different centers to utilize artificial intelligence (AI)/machine learning to quantify pathologic findings such as amyloid plaques and tangles in neurodegenerative diseases. However, the lack of access to a whole-slide scanner has incumbered our progress. Meanwhile, as the expectation was created, now we have increasing demand for a service we are unable to properly deliver and did not include its cost in submitted P30 budget. Secondly, we realize a need to extend our effort to collect postmortem cerebrospinal fluid to address increasing demand for this valuable specimen and assist our biomarker core for their biomarker studies. Neuropathology Core activities continue to rapidly grow, requiring a more comprehensive formal database program to support a higher level of Core documentation and inventory performance. Finally, we underestimated the rate of degeneration in our essential equipment such as tissue processor and microtome and their lack of extended technical support from their manufacturers for the coming years. For sustainable growth, we must replace them with a new reliable machine to be functional and supported for at least the next 10 years.

项目概要本补充要求提供一年的资金来支持与大学相关的不可预见的费用堪萨斯阿尔茨海默病研究中心 (KU ADRC) 神经病理学 (NP) 核心。神经病理学核心与其他 ADRC 核心协作提供关键的 KU ADRC 基础设施（图 1）。神经病理学核心功能对于中心的使命和科学主题至关重要 1)。维持尸检计划和脑库；2）提供神经病理学诊断、咨询和对研究人员和受训人员进行培训；3) 获取诱导多能干细胞的硬脑膜和皮肤活组织检查 (IPSC) 系；4) 为脑组织提供 Biomarker Core 进行线粒体 DNA (mtDNA) 测序。 NP 核心不断升级其状态以适应该领域的诊断进展，并根据此外，核心还批准并接受了新的指导方针、知识和最先进的技术。参与产生这些进步的重要辩论。请求 NP Core 支持的调查人员数量出现意外增长，这是一种突发的不可预见的情况条件限制了我们的核心创建载玻片数字图书馆，增加了对死后脑脊液的需求样本和我们需要更换的重要设备的意外过时，预示着这一要求。我们在最近资助的 P30 中提议向计算机提供全幻灯片扫描图像的开放库然而，科学家和其他研究人员使用位于病理学部门的扫描仪。该部门对肿瘤和其他病理标本的需求不断增加，但我们无法满足正确使用此服务，因为当前扫描仪速度很慢并且该部门无法分配适当的所需时间另一方面，我们已经开始与不同领域的计算机科学家合作。中心利用人工智能 (AI)/机器学习来量化淀粉样蛋白等病理结果然而，由于缺乏全玻片扫描仪，导致神经退行性疾病中的斑块和缠结。同时，随着期望的产生，我们对未来的需求也越来越大。我们无法正确提供服务，并且未将其成本纳入提交的 P30 预算中。认识到有必要加大力度收集死后脑脊液，以满足日益增长的需求这个有价值的样本可以帮助我们的生物标志物核心进行生物标志物研究。神经病理学核心活动持续快速增长，需要更全面的正式数据库最后，我们低估了支持更高水平的核心文档和库存绩效的计划。我们的基本设备（例如组织处理机和切片机）的退化速度及其缺乏为了未来几年的可持续增长，我们必须获得制造商的长期技术支持。用新的可靠机器替换它们，以便至少在未来 10 年内发挥作用并获得支持。

项目成果

期刊论文数量（69）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Sex Differences in Resilience and Resistance to Brain Pathology and Dysfunction Moderated by Cerebrovascular Response to Exercise and Genetic Risk for Alzheimer's Disease.

DOI：
10.3233/jad-220359
发表时间：
2022
期刊：
JOURNAL OF ALZHEIMERS DISEASE
影响因子：
4
作者：
Palmer, Jacqueline A.;Kaufman, Carolyn S.;Vidoni, Eric D.;Honea, Robyn A.;Burns, Jeffrey M.;Billinger, Sandra A.
通讯作者：
Billinger, Sandra A.

Rationale and methods for a multicenter clinical trial assessing exercise and intensive vascular risk reduction in preventing dementia (rrAD Study).

评估运动和强化血管风险降低预防痴呆症的多中心临床试验的基本原理和方法（rrAD 研究）。

DOI：
10.1016/j.cct.2019.02.007
发表时间：
2019
期刊：
Contemporary clinical trials
影响因子：
2.2
作者：
Szabo-Reed,AmandaN;Vidoni,Eric;Binder,EllenF;Burns,Jeffrey;Cullum,CMunro;Gahan,WilliamP;Gupta,Aditi;Hynan,LindaS;Kerwin,DianaR;Rossetti,Heidi;Stowe,AnnM;Vongpatanasin,Wanpen;Zhu,DavidC;Zhang,Rong;Keller,JeffreyN
通讯作者：
Keller,JeffreyN

In Vivo Brain Delivery and Brain Deposition of Proteins with Various Sizes.

不同大小蛋白质的体内脑输送和脑沉积。