Time-sensitive Collection and Analysis of a Marine-Derived Toxic Event

海洋源性有毒事件的时间敏感收集和分析

基本信息

批准号：
8745126
负责人：
THERESA GAASTERLAND
金额：
$ 19.38万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-01-20 至 2017-01-19
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8745126
关键词：
Address Adverse effects Affect Algae Algal Blooms Applications Grants Aquaculture Archives Bio-Base Biological Assay California Cataloging Catalogs Cell Culture Techniques Cell physiology Cells Chemistry Collection Coupled DNA DNA Sequence Data Databases Dinophyceae Early Diagnosis Evaluation Event Fishes Fractionation Frequencies Future Gene Expression Gene Expression Profile Genes Genome Genomic Library Genomics Geographic Locations Goals Habitats Health High-Throughput Nucleotide Sequencing Human Human Cell Line Indigenous Knowledge Libraries Longitudinal Studies Marines Mass Spectrum Analysis Measures Metagenomics Methods Microbe Organism Oxygen Personal Satisfaction Phytoplankton Pollution Procedures Production Proxy RNA RNA Sequences Relative (related person)Research Resources Risk Sampling Technology Testing Time Toxic effect Toxin Water Work analytical method anthropogenesis base comparative cost cytotoxicity harmful algal blooms in vitro Assay innovation metabolomics neurotoxicity next generation sequencing ocean ecosystems public health relevance response transcriptomics

项目摘要

DESCRIPTION (provided by applicant): Time-sensitive Collection and Analysis of a Marine-Derived Toxic Event Harmful algal blooms (HABs) impact many coastal states over large geographic areas and threaten environmental and socio-economical resources as well as human health and well-being. This project aims to use pre-bloom, bloom and post-bloom filtered samples from the current, September 2013, southern California bloom of Lingulodinium polyedrum to create a reference genome and transcriptome database, and assess toxin production and effect in human cell assays. The unpredictable timing of these blooms makes the current bloom event an opportunity to collect concentrated samples to evaluate potential harm to human health during these episodic events. The samples will be used for DNA and RNA sequencing and for secondary metabolite extractions, the information created will be placed in a reference library as the basis for further examination of archived samples. As part of ongoing collections, physical parameters will be measured and integrated with the genomic, transcriptomic and secondary metabolomic reference library to further aid in interpreting the data. Aim 1 describes the collections made and collections currently being performed. Aim 2 addresses the extraction, sequencing, assembly and annotation of DNA and RNA from these samples. This information will be coupled with a chemistry effort (Aim 3a) where samples will be investigated for their production of secondary metabolites (potential toxins, including sub-lethal) using mass spectrometry and NMR methods. The isolated secondary metabolites will be evaluated in four phenotypic in vitro assays used as a proxy for their potential cytotoxicity, neuro- and immuno-modulation even at sub-lethal levels (Aim 3b). In total, the work will provide fundamental knowledge by creating a reference library that will aid in further examination of archived samples collected since 2008, containing both bloom and non-bloom samples. This collected information is essential for evaluation of mitigation strategies and to provide early detection of toxic events in order to limit the impacts on human health, and will serve as the basis of an R01 grant application to be submitted next year.

描述（由申请人提供）：海洋衍生有毒事件的时间敏感收集和分析有害藻华 (HAB) 影响大片地理区域的许多沿海国家，威胁环境和社会经济资源以及人类健康和福祉。存在。该项目旨在利用2013年9月南加州多枝藻花的开花前、开花和开花后过滤样本来创建参考基因组和转录组数据库，并评估人类细胞分析中的毒素产生和效果。这些水华的时间不可预测，使得当前的水华事件成为收集浓缩样本的机会，以评估这些偶发事件对人类健康的潜在危害。这些样本将用于 DNA 和 RNA 测序以及次级代谢物提取，所创建的信息将放入参考库中，作为进一步检查存档样本的基础。作为正在进行的收集的一部分，将测量物理参数并将其与基因组、转录组和次级代谢组参考库集成，以进一步帮助解释数据。目标 1 描述了已进行的收集和当前正在执行的收集。目标 2 涉及从这些样本中提取、测序、组装和注释 DNA 和 RNA。这些信息将与化学工作（目标 3a）结合起来，其中将研究样品的次级代谢物（潜在毒素，包括亚致死毒素）的产生使用质谱和核磁共振方法。分离的次级代谢物将在四种表型体外测定中进行评估，用作其潜在细胞毒性、神经和免疫调节的代理，甚至在亚致死水平下也是如此（目标 3b）。总的来说，这项工作将通过创建一个参考库来提供基础知识，该库将有助于进一步检查自 2008 年以来收集的存档样本，其中包含水华和非水华样本。收集到的信息对于评估缓解策略和早期发现有毒事件以限制对人类健康的影响至关重要，并将作为明年提交的 R01 拨款申请的基础。