TNPRC Regional Biocontainment Laboratory Upgrade for Capacity Building

TNPRC 区域生物防护实验室升级以进行能力建设

• 批准号: 10632933
• 负责人: JAY RAPPAPORT
• 金额: $ 344.3万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-23 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10632933
• 关键词: 2019-nCoV; Appointment; COVID-19; COVID-19 pandemic; Caring; Centers for Disease Control and Prevention (U.S.); Communicable Diseases; Communities; Containment; Decontamination; Detection; Development; Emerging Communicable Diseases; Ensure; Epidemic; Equipment; Faculty; Flow Cytometry; Funding; Future; Goals; Growth; Health; Human; IACUC; Immunology; Infrastructure; Joints; Laboratories; Liquid substance; Mission; Modernization; Outcome; Power Sources; Primates; Public Health; Pulmonology; Regulation; Research; Research Activity; Research Personnel; Resources; Safety; Series; Services; Site; System; Talents; Therapeutic; Tissue Sample; Toxin; Tropical Medicine; United States National Institutes of Health; Update; Vaccines; Validation; Work; animal model development; animal resource; biodefense; diagnostic assay; emerging pathogen; exhaust; improved; medical schools; multidisciplinary; nonhuman primate; novel diagnostics; operation; pathogen; population health; product development; programs; quality assurance; repaired; research clinical testing; response; restoration; risk minimization; virology; wasting

The Tulane National Primate Research Center (TNPRC) is one of seven NPRCs that serve as local, regional, and national resources to conduct nonhuman primate (NHP) research with the goal of improving human health. The TNPRC maintains a focus on infectious diseases and is the only NPRC with a Regional Biocontainment Laboratory (RBL) at biosafety level 3 (BSL-3) for the study of high consequence pathogens, including select agents and toxins, and biodefense research. In recent years, and particularly with the onset of the COVID-19 pandemic, the TNPRC RBL has seen massive growth in emerging pathogen and biodefense work. The facility is in critical need of additional upgrades, modernization projects, and laboratory enhancements, both to support the rapidly growing demand for BSL-3 research and to ensure a robust and efficient containment system that is vital for protecting workers, the Center, and the surrounding community. The purpose of the proposed project is to strategically enhance research capabilities by introducing new equipment space within the TNPRC RBL to accommodate our growing BSL-3 research portfolio. To this end, we propose the following specific aims: Aim 1. Implement a series of improvements to the TNPRC RBL infrastructure, including replacement of damaged phoenix valves, restoration of DeCon Tank capabilities, upgrade of uninterruptible power supply (UPS) systems, and update of the boiler burner which can no longer be repaired due to the discontinued manufacturing of replacement parts. Aim 2. Upgrade equipment to expand clinical evaluation capabilities in the BSL-3. Aim 3. Incorporate functional equipment pieces to support BSL-3 research activities, enabling and expanding the capacity of the RBL to conduct numerous research capabilities where regulations are extensive to be able to take them into non-select agent space. The ability to respond to potential epidemics rapidly and efficiently by developing and applying novel diagnostic assays, therapeutics, and vaccines is critical to minimize risk and negative population health outcomes. The TNPRC is uniquely capable of quickly responding to emerging threats, from detection through product development, due to our status as the only NPRC with an on-site BSL-3 Regional Biocontainment Laboratory, unique Quality Assurance Program, and extensive support of on-site core services. By mid-February 2020, we had already established a COVID-19 research program, with Centers for Disease Control & Prevention (CDC)-permitted acquisition of SARS-CoV-2 stock and IACUC approval of our animal model development studies. The TNPRC has a highly talented group of investigators with nationally recognized expertise in biocontainment, virology, immunology, pulmonology, vaccines, and veterinary care. Our faculty, with joint appointments in Departments across the Tulane Schools of Medicine and Public Health & Tropical Medicine, are involved in the development of multiple collaborative planning efforts locally, regionally, and nationally, including a Tulane-funded multidisciplinary Center of Excellence for Emerging/Re- emerging Infectious Disease (CEERID) and an application for federal funding of a Center of Excellence for Emerging Pathogens. Evidence of the impact of our collaborative research efforts is demonstrated by the allocation of close to 75% of animal resources to affiliate investigators and distribution of nearly 90,000 tissue samples to 461 NIH-funded collaborators in a recent 5-year period. This broad network of investigators will rely on the proposed project to enhance the capability and quality of our work and mission in support of studies directed toward the safe and rapid response to high consequence pathogens.

杜兰国家灵长类动物研究中心 (TNPRC) 是七个 NPRC 之一，作为当地、 区域和国家资源进行非人类灵长类动物（NHP）研究，其目标是 改善人类健康。 TNPRC 始终关注传染病，并且是唯一的 NPRC 拥有生物安全 3 级 (BSL-3) 的区域生物防护实验室 (RBL)，用于研究高浓度 病原体的后果，包括选择制剂和毒素，以及生物防御研究。近来 多年来，特别是随着 COVID-19 大流行的爆发，TNPRC RBL 发生了大规模的 新兴病原体和生物防御工作的增长。该设施迫切需要额外的 升级、现代化项目和实验室增强，都是为了支持快速增长的 满足 BSL-3 研究的需求，并确保建立一个强大而高效的遏制系统，这对于 保护工人、中心和周边社区。拟议项目的目的 是通过在实验室内引入新的设备空间来战略性地增强研究能力 TNPRC RBL 旨在适应我们不断增长的 BSL-3 研究组合。为此，我们建议 以下具体目标： 目标 1. 对 TNPRC RBL 基础设施实施一系列改进，包括更换 损坏的菲尼克斯阀门、恢复 DeCon Tank 功能、升级不间断电源 供应（UPS）系统，以及由于无法再维修的锅炉燃烧器的更新 停止生产替换零件。 目标 2. 升级设备以扩展 BSL-3 的临床评估能力。 目标 3. 整合功能设备以支持 BSL-3 研究活动，从而实现和 扩大 RBL 的能力，使其能够在监管不力的情况下开展大量研究 广泛到能够将他们带入非选择代理空间。 通过开发和应用新颖的方法快速有效地应对潜在流行病的能力 诊断分析、治疗方法和疫苗对于最大限度地减少风险和阴性人群至关重要 健康结果。 TNPRC 具有独特的能力，能够快速响应新出现的威胁， 由于我们是唯一拥有现场 BSL-3 的 NPRC，因此可以通过产品开发进行检测 区域生物防护实验室、独特的质量保证计划以及广泛的支持 现场核心服务。到 2020 年 2 月中旬，我们已经建立了 COVID-19 研究 计划，经疾病控制与预防中心 (CDC) 许可获取 SARS-CoV-2 库存和 IACUC 批准我们的动物模型开发研究。 TNPRC 拥有高度 才华横溢的研究人员团队，在生物遏制、病毒学、 免疫学、肺病学、疫苗和兽医护理。我们的教职员工联合任命 杜兰医学院和公共卫生与热带医学学院的各个部门是 参与当地、区域和地区的多项协作规划工作的制定 在全国范围内，包括杜兰大学资助的新兴/再创新多学科卓越中心 新发传染病 (CEERID) 和联邦资助中心的申请 卓越的新兴病原体。我们合作研究工作的影响的证据是 近 75% 的动物资源分配给附属研究人员和 近 5 年内向 461 名 NIH 资助的合作者分发了近 90,000 个组织样本 时期。这个广泛的研究人员网络将依靠拟议的项目来 增强能力 我们的工作质量和使命，以支持针对安全和快速反应的研究 对高后果病原体。