Structural Biology

结构生物学

基本信息

批准号：
8340243
负责人：
KEVIN J. CULLEN
金额：
$ 7.15万
依托单位：
UNIVERSITY OF MARYLAND BALTIMORE
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-24 至 2016-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8340243
关键词：
Accounting Address Adopted Architecture Basic Science Biological Cancer Center Cancer Center Support Grant Cancer Etiology Commit Computers and Advanced Instrumentation Consultations Crystallization Crystallography Data Data Collection Defect Drug Design Effectiveness Environment Event Experimental Designs Goals Ice Institution Instruction Macromolecular Complexes Malignant Neoplasms Maryland Methods Mission Molecular Molecular Structure Nuclear Magnetic Resonance Nucleic Acids Principal Investigator Process Protein Conformation Proteins Research Research Personnel Robot Sampling Services Solutions Structure System Techniques Technology Therapeutic Intervention Time United States Universities Work X-Ray Crystallography base combat design instrument macromolecule nano programs protein complex research study structural biology three dimensional structure tool

项目摘要

PROJECT SUMMARY (See instructions): The Structural Biology Shared Sen/ice (SBSS) helps researchers use the unique information derived from macromolecular structures to understand the molecular basis of cancer-causing cellular defects and to design drugs that mitigate such defects. SBSS comprises the X-ray Crystallography Shared Service (XRSS) and Nuclear Magnetic Resonance Shared Service (NMRSS). Together, these two facilities offer different technical approaches that work in synergy to allow researchers at the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) to view cancer-related biological macromolecules at the molecular level. Researchers from UMGCC account for more than one-half of the use of SBSS. Drs. David Weber and Eric Toth advise UMGCC investigators on how best to use SBSS to advance the goals of their project. To aid in this process, the SBSS has adopted an experimental pipeline that allows UMGCC investigators to determine rapidly which experimental technique best suits their needs and proceed with the experimental design that provides the most useful data in the shortest amount of time. The x-ray component of SBSS gives UMGCC investigators access to an Oryx Nano crystallization robot and a powerful Rigaku data collection system. These instruments provide UMGCC investigators with the tools to produce rapidly diffraction-quality crystals of cancer-related macromolecules and determine their three-dimensional structures. The NMR component ofthe SBSS currently provides access to both 600- and 800-MHz spectrometers. By April 2011, the NMR facility will acquire and install a 950-MHz spectrometer, the only such instrument at an academic institution in the United States. This array of cutting-edge spectrometers will allow UMGCC researchers to access the most sophisticated techniques for determining solution structures and examining the dynamics of large cancer-related macromolecules. Using these technologies, SBSS has supplied a number of UMGCC researchers with key experimental data that greatly enhanced their research programs.

项目摘要（参见说明）：结构生物学共享 Sen/ice (SBSS) 帮助研究人员利用从大分子结构中获得的独特信息来了解致癌细胞缺陷的分子基础，并设计出减轻此类缺陷的药物。 SBSS包括X射线晶体学共享服务（XRSS）和核磁共振共享服务（NMRSS）。这两个设施共同提供不同的协同作用的技术方法使马里兰大学马琳和斯图尔特格林鲍姆癌症中心 (UMGCC) 的研究人员能够在分子水平上观察与癌症相关的生物大分子。 UMGCC 的研究人员占 SBSS 使用量的一半以上。博士。 David Weber 和 Eric Toth 就如何最好地使用 SBSS 来推进他们的目标向 UMGCC 调查人员提供建议项目。为了帮助这一过程，SBSS 采用了实验流程，使 UMGCC 研究人员能够快速确定哪种实验技术最适合他们的需求，并继续进行实验设计，以在最短的时间内提供最有用的数据。 SBSS 的 X 射线组件使 UMGCC 调查人员能够接触到 Oryx Nano 结晶机器人和强大的 Rigaku 数据收集系统。这些仪器为 UMGCC 研究人员提供了快速生成癌症相关大分子衍射质量晶体并确定其三维结构的工具。 SBSS 的 NMR 组件目前提供对 600 MHz 和 800 MHz 光谱仪的访问。到 2011 年 4 月，NMR 设施将购买并安装一台 950 MHz 光谱仪，这是美国学术机构中唯一的此类仪器。这一系列尖端光谱仪将使 UMGCC 研究人员能够利用最先进的技术来确定溶液结构并检查与癌症相关的大分子的动力学。利用这些技术，SBSS 为许多 UMGCC 研究人员提供了关键实验数据，极大地增强了他们的研究项目。