Flow Cytometry

流式细胞术

• 批准号: 8340241
• 负责人: KEVIN J. CULLEN
• 金额: $ 5.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-24 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8340241
• 关键词: Accounting; Applied Research; Area; Basic Science; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cell Separation; Consultations; Data Analyses; Disease; Effectiveness; Equipment; Flow Cytometry; Funding; Goals; Ice; Individual; Instruction; Krypton; Lasers; Malignant Neoplasms; Maryland; Mission; National Cancer Institute; Principal Investigator; Process; Publications; Research; Research Personnel; Services; Sorting - Cell Movement; System; Technical Expertise; Technology; Training; Universities; Virus Diseases; analog; base; design; digital; innovation; instrument; member; novel; oncology; operation; programs; research study; stem cell biology; ultraviolet

PROJECT SUMMARY (See instructions); The Flow Cytometry Shared Service (FCSS) at the University of Maryland Marlene and Stewart Greenebaum Cancer Center (UMGCC) offers flow cytometry equipment and technical expertise to UMGCC members conducting research in cancer biology and other areas of basic and applied science. Services are open to the entire University of Maryland campus, but UMGCC accounts for about 75 percent of the total use. FCSS provides full scale, state-of-the-art flow cytometry services, from functional and phenotypic analysis to cell sorting. Analytical instruments include a modern, fully digital, 3-laser Becton Dickinson (BD) LSR II flow cytometer, and two analog flow cytometers: a BD LSR 1 and a BD FACScan instrument. Cell sorting is conducted with (1) a BD FACSAria I digital instrument equipped with two lasers and seven-colordetection capability and (2) a BD FACSVantage SE instrument upgraded with BD's Digital Acquisition System (DIVA) and equipped with three lasers including a tunable krypton laser for 351 nm ultraviolet excitation. FCSS also offers users both operator-assisted and self-service data analysis on several platforms as well as experimental training and consultation to support project design for individual analytical or sorting experiments. The effectiveness of FCSS is evident from the steady increase of its utilization in recent years, the more than 50 principal investigators who are using the shared service, and the number of publications and National Cancer Institute-funded projects supported by experiments conducted with the help ofthe shared service. The goal of FCSS is to further increase the breadth and scope of its services by expanding cell-sorting capabilities; supporting novel, flow cytometry-based analytical applications; and strengthening cooperation with other UMGCC shared sen/ices to help introduce innovative, cutting-edge technologies to UMGCC investigators.

项目摘要（参见说明）； 马里兰大学 Marlene 和 Stewart Greenebaum 癌症中心 (UMGCC) 的流式细胞术共享服务 (FCSS) 为开展癌症生物学以及其他基础和应用科学领域研究的 UMGCC 成员提供流式细胞术设备和技术专业知识。服务向整个马里兰大学校园开放，但 UMGCC 约占总使用量的 75%。 FCSS 提供全面、最先进的流式细胞术服务，从功能和表型分析到细胞分选。分析仪器包括现代化的全数字 3 激光 Becton Dickinson (BD) LSR II 流式细胞仪和两台模拟流式细胞仪：BD LSR 1 和 BD FACScan 仪器。使用 (1) 配备两个激光器和七色检测的 BD FACSAria I 数字仪器进行细胞分选 (2) BD FACSVantage SE 仪器升级了 BD 的数字采集系统 (DIVA)，并配备了三个激光器，包括用于 351 nm 紫外线激发的可调谐氪激光器。 FCSS 还在多个平台上为用户提供操作员辅助和自助数据分析，以及实验培训和咨询，以支持单个分析或分类实验的项目设计。 FCSS 的有效性从近年来其利用率的稳步增长、使用共享服务的 50 多名主要研究人员以及在 FCSS 的帮助下进行的实验支持的出版物和国家癌症研究所资助项目的数量中可见一斑。共享服务。 FCSS的目标是通过扩大细胞分选能力来进一步扩大其服务的广度和范围；支持新颖的、基于流式细胞术的分析应用；加强与其他 UMGCC 共享服务的合作，帮助向 UMGCC 调查人员介绍创新、尖端技术。