Role of anterior olfactory nucleus for multi-sensory integration in the olfactory system

前嗅核在嗅觉系统多感觉整合中的作用

• 批准号: 10589501
• 负责人: Thomas A Cleland
• 金额: $ 4.66万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10589501
• 关键词: Anterior; Behavioral; Brain; Data; Decision Making; Electrophysiology (science); Exhibits; Hippocampus (Brain); Immediate-Early Genes; Label; Lead; Learning; Measurement; Mental Health; Odors; Olfactory Pathways; Patients; Perception; Play; Post-Traumatic Stress Disorders; Process; Research; Rodent; Role; Sensory; Signal Transduction; Structure; System; Testing; Time; Trauma; awake; base; desensitization; maladaptive behavior; multisensory; olfactory bulb; olfactory nuclei; optogenetics; piriform cortex; response; spatial integration

Project Summary Sensory signals encountered under different circumstances may have quite different implications. In the early olfactory system, preliminary evidence suggests that this (non-olfactory) contextual information is integrated into odor representations at a very early stage, potentially even the main olfactory bulb. Recent evidence indicates that the anterior olfactory nucleus (AON), a structure directly adjoining the olfactory bulb, serves to integrate afferent odor information with contextual information from the ventral hippocampus (vHC) and is necessary to solve contextually-dependent olfactory decision-making tasks. The vHC is known to relay task-relevant spatial contextual information to other brain systems. We here hypothesize that direct projections from the vHC to the AON play a dominant role in the integration of contextual and olfactory information, and that the AON embeds this multisensory contextual information into early-stage odor representations. Our preliminary data show that rodents can learn to respond differently to odors based on the spatial context in which they are encountered, and that the expression of such a rule depends on both AON and vHC, whereas a similar but odor-independent task requires vHC but not AON. We propose a multipronged approach to understanding the integration of spatial context into olfactory representations, engaging electrophysiological ensemble recordings and interareal coherence measurements in awake, behaving rodents, the optogenetic manipulation of vHC and AON circuit activities, and a double-labeling strategy for the within-subjects comparison of immediate-early gene (Fos) responses across two experimental conditions separated in time. Supplement summary: Piriform cortex (PC), is tightly interconnected with OB, AON and vHC and potentially involved in similar processed. The additional Aims planned include testing the hypothesis that PC performs complementary functions to AON and is involved in contextual processing.

项目概要 在不同情况下遇到的感官信号可能会产生截然不同的含义。在 早期嗅觉系统，初步证据表明这种（非嗅觉）上下文信息是 在很早的阶段就融入到气味表征中，甚至可能融入到主嗅球中。最近的 有证据表明前嗅核（AON）是直接毗邻嗅球的结构， 用于将传入的气味信息与来自腹侧海马的上下文信息整合起来 （vHC）并且对于解决上下文相关的嗅觉决策任务是必要的。 众所周知，vHC 会将与任务相关的空间上下文信息传递给其他大脑系统。我们在这里 假设从 vHC 到 AON 的直接投影在整合中起主导作用 上下文和嗅觉信息，并且 AON 将这种多感官上下文信息嵌入到 早期气味表征。我们的初步数据表明，啮齿类动物可以学会对以下情况做出不同的反应： 气味基于它们遇到的空间环境，并且这种规则的表达 取决于 AON 和 vHC，而类似但与气味无关的任务需要 vHC 而不是 AON。 我们提出了一种多管齐下的方法来理解空间背景与嗅觉的整合 表征、参与电生理学合奏记录和区域间一致性 清醒、行为啮齿类动物的测量、vHC 和 AON 电路活动的光遗传学操作， 以及用于受试者内立即早期基因（Fos）反应比较的双标记策略 跨越两个时间间隔的实验条件。 补充摘要：梨状皮层（PC），与 OB、AON 和 vHC 紧密相连， 可能涉及类似的处理。计划的其他目标包括测试 PC 的假设 执行 AON 的补充功能并参与上下文处理。