Cochlear mechanics in the mouse

小鼠的耳蜗力学

基本信息

批准号：
10614068
负责人：
John S Oghalai
金额：
$ 62.33万
依托单位：
UNIVERSITY OF SOUTHERN CALIFORNIA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-01 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10614068
关键词：
3-Dimensional Affect Air Anatomy Anesthesia procedures Animals Arousal Auditory Auditory system Back Basic Science Basilar Membrane Biomechanics Biomedical Engineering Biophysical Process Brain Characteristics Clinical Cochlea Cochlear duct Compensation Complex Data Dependence Diameter Environment External auditory canal Frequencies Hair Cells Head Hearing Hearing Aids Image Left Mammals Measurement Measures Mechanics Medial Mediating Motion Mus Optical Coherence Tomography Optics Organ of Corti Outer Hair Cells Painless Physiology Process Production Pupil Reflex action Research Project Grants Resolution Role Speech Structure Supporting Cell System Technology Testing Time Travel Walking Wild Type Mouse Work auditory stimulus awake bone capsule design experimental study hearing impairment in vivo indexing innovation otoacoustic emission peer pressure response restraint sound stem tectorial membrane treadmill vector vibration walking speed

项目摘要

Project Summary/Abstract Sound pressure produces force across the mammalian cochlear partition, ultimately creating a vibratory traveling wave that propagates longitudinally up the cochlear duct. The key feature distinguishing this process from the non-mammalian cochlea is amplification, whereby forces produced by thousands of outer hair cells (OHCs) sharpen and amplify the traveling wave. Our overarching objective is to understand how the complex biomechanics of the 3D multi-cellular and acellular arrangement that form the organ of Corti work together to create cochlear amplification. Specifically, we will determine how this process, which stems from the broadly- tuned basilar membrane, creates sharp frequency tuning and high sensitivity. This question is significant on a basic science level because these biophysical processes underlie the ability to hear sounds just above the Brownian motion of molecules in air with an exquisite frequency resolution. This question remains unsolved and is clinically important because hearing loss is typically due to loss of cochlear amplification. Our central hypothesis is that, beyond the broad tuning provided by basilar membrane mechanics, the forces produced by OHCs are also tuned by additional mechanisms. In aim 1, we will use 3D Volumetric Optical Coherence Tomography and Vibrometry (VOCTV) in mice to test whether the forces produced by OHCs are tuned by the mechanics of the supporting cells and acellular structures that form the organ of Corti. In aim 2, we will use 1D VOCTV in awake behaving mice to test whether cochlear amplification is modulated by brain state via the medial olivocochlear efferent (MOC) system by varying OHC force production. Together, these data will be interpreted so as to test our hypothesis. If our hypothesis is true, sharply-tuned differential motion within the organ of Corti is necessary to generate the sensitivity and sharp tuning of the mammalian cochlea and brain state modulates cochlear amplification via the MOC efferent system.

项目摘要/摘要音压会在哺乳动物的人工耳蜗上产生力，最终产生振动纵向传播的行驶波在人工耳蜗上传播。区分此过程的关键功能从非哺乳动物的耳蜗中是放大的，从而由数千个外毛细胞产生的力（OHC）锐化并扩大行驶波。我们的总体目标是了解复杂形成Corti器官的3D多细胞和细胞排列的生物力学创建耳蜗放大。具体而言，我们将确定这一过程是如何源于广泛的调谐的基底膜，会产生清晰的频率调整和高灵敏度。这个问题对基础科学层面是因为这些生物物理过程的能力是在空气中分子的布朗运动，具有精致的频率分辨率。这个问题仍未解决并且在临床上很重要，因为听力损失通常是由于人工耳蜗扩增的丧失。我们的中心假设是，除了基底膜力学提供的广泛调整之外，由 OHC还通过其他机制调节。在AIM 1中，我们将使用3D体积光学连贯性小鼠中的断层扫描和振动法（VOCTV）测试OHC产生的力是否由形成Corti器官的支撑细胞和细胞结构的力学。在AIM 2中，我们将使用1D 在醒着的行为小鼠中的voctv，以测试人工耳蜗放大是否通过大脑状态调节内侧橄榄石传出（MOC）系统通过改变OHC力的产生。在一起，这些数据将是解释以检验我们的假设。如果我们的假设是真实的，在 Corti的器官对于产生哺乳动物耳蜗和大脑的灵敏度和尖锐调整是必要的状态通过MOC传出系统调节人工耳蜗。

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Hair cell force generation does not amplify or tune vibrations within the chicken basilar papilla.

DOI：
10.1038/ncomms13133
发表时间：
2016-10-31
期刊：
NATURE COMMUNICATIONS
影响因子：
16.6
作者：
Xia, Anping;Liu, Xiaofang;Raphael, Patrick D.;Applegate, Brian E.;Oghalai, John S.
通讯作者：
Oghalai, John S.

Rules for Successful Leadership in Otolaryngology-Head and Neck Surgery.

耳鼻喉头颈外科成功领导规则。

DOI：
10.1002/lary.30052
发表时间：
2022
期刊：
The Laryngoscope
影响因子：
0
作者：
Oghalai,JohnS
通讯作者：
Oghalai,JohnS

Controlled ultrasound tissue erosion.

受控超声组织侵蚀。

DOI：
10.1109/tuffc.2004.1308731
发表时间：
2004
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
0
作者：
Xu,Zhen;Ludomirsky,Achiau;Eun,LucyY;Hall,TimothyL;Tran,BinhC;Fowlkes,JBrian;Cain,CharlesA
通讯作者：
Cain,CharlesA