Analytical Cell Models Core

分析单元模型核心

基本信息

批准号：
10608972
负责人：
STEPHEN A DUNCAN
金额：
$ 25.07万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-15 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10608972
关键词：
Adopted Affect Animal Model CRISPR/Cas technology Cell Culture Techniques Cell Differentiation process Cell Line Cell Maintenance Cell Separation Cell model Cells Cytometry DNA Sequence Alteration Digestive System Disorders Ensure Evaluation Flow Cytometry Gastrointestinal tract structure Generations Genetic Variation Genome engineering Goals Hepatocyte Human Infrastructure Laboratories Liver Liver diseases Medical Modeling Mutate Mutation Organoids Pathogenesis Patients Pluripotent Stem Cells Procedures Production Protocols documentation Reagent Research Research Personnel Rodent Running South Carolina Technology Testing Tissues Training Universities Update Wisconsin analytical tool cholangiocyte differentiation protocol established cell line experience genome editing human model induced pluripotent stem cell injured medical schools member new technology professor programs stem cell biology tissue culture tool

项目摘要

Analytical Cell Models Core - Abstract The DDRCC Analytical Cell Models Core proposes to provide DDRCC members with access to state-of-the-art tissue culture models of digestive and liver disease. We will use genome engineering technologies including CRIPSR-Cas9 to introduce genetic alterations into established cell lines and into induced pluripotent stem cells (iPSCs). The iPSCs will be differentiated to form cells of the gastrointestinal tract and liver that will be used for study by DDRCC members. The Core will also provide members with primary parenchymal and non- parenchymal livers cells from both human and rodent livers as well as critical tools for the analyses of cells. In addition to providing DDRCC members with cell lines that can be used for direct analyses, the Core will also provide members with training and critical reagents that will allow them to establish many of the procedures in their own laboratories. The Core will adopt a program of rigorous evaluation that will ensure that it performs optimally and continues to provide DDRCC members access to new technologies as they are developed. Dr. Duncan, who will lead the core, is an expert in stem cell biology and cell differentiation, and he has used genome engineering extensively to generate cells to model liver disease. He has previous experience in running a similar core while a Professor at the Medical College of Wisconsin. He will be supported by Dr. Don Rockey who has extensive experience in the isolation of cells from both human and rodent livers.

分析细胞模型核心 - 摘要 DDRCC分析细胞模型核心建议为DDRCC成员提供最新的访问权限消化和肝病的组织培养模型。我们将使用基因组工程技术，包括 CRIPSR-CAS9将遗传改变引入已建立的细胞系并诱导的多能干细胞（IPSC）。 IPSC将被区分以形成胃肠道和肝脏的细胞 DDRCC成员的研究。核心还将为成员提供原发性实质和非 - 来自人和啮齿动物肝的实质肝细胞以及细胞分析的关键工具。在除了向DDRCC成员提供可用于直接分析的单元线，核心还将为成员提供培训和关键试剂，使他们能够在他们自己的实验室。核心将采用严格的评估计划，以确保其执行最佳，并继续为DDRCC成员提供开发新技术的访问。博士将领导核心的邓肯（Duncan）是干细胞生物学和细胞分化的专家，他使用了基因组广泛的工程以产生细胞以建模肝病。他以前有运行类似的经验核心威斯康星州医学院的教授。他将得到拥有的Don Rockey博士的支持从人和啮齿动物肝脏分离细胞的丰富经验。