Cell Models Core

细胞模型核心

基本信息

批准号：
10337320
负责人：
STEPHEN A DUNCAN
金额：
$ 24.28万
依托单位：
MEDICAL UNIVERSITY OF SOUTH CAROLINA
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-03 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10337320
关键词：
Adopted Affect Animal Disease Models Biological Models CRISPR/Cas technology Cell Culture Techniques Cell Differentiation process Cell Line Cell Maintenance Cell model Cells Centers of Research Excellence Communities DNA Sequence Alteration Data Development Digestive System Disorders Drug Screening Ensure Enteroendocrine Cell Epithelial Cells Evaluation Feedback Funding Future Gastrointestinal tract structure Generations Genes Genome engineering Goals Goblet Cells Hepatocyte Human Implant Investigation Laboratories Lead Leadership Liver Liver diseases Medical Mentors Mesenchyme Modeling Molecular Mutate Mutation Needs Assessment Organoids Paneth Cells Performance Printing Procedures Production Program Evaluation Protocols documentation Reagent Reproducibility Research Research Personnel Resources Running Self-Examination Services South Carolina Surveys System Technical Expertise Technology Time Tissues Training Universities Wisconsin Work base cell type cholangiocyte directed differentiation established cell line experience experimental study genetically modified cells human pluripotent stem cell in vivo induced pluripotent stem cell innovation intestinal epithelium medical schools mouse model new technology operation professor programs recruit stable cell line stem cell biology stem cells tissue culture tool training opportunity

项目摘要

Cell Models Core – Project Summary The COBRE in Digestive & Liver Disease (CDLD) Cell Models Core will provide CDLD investigators with access to state-of-the-art tissue culture models of digestive and liver disease. We will use genome engineering technologies including CRIPSR-Cas9 to introduce specific genetic alterations into established cell lines and into induced pluripotent stem cells (iPSCs). The iPSCs will be differentiated to form cells of the gastrointestinal tract and liver that will be used for study by CDLD investigators. In addition to providing CDLD investigators cell lines and organoids that can be used for direct analyses, the Cell Models Core will also equip investigators with mentoring, training, and critical reagents allowing them to establish the procedures in their own laboratories. Through training opportunities, the Core will advance the technological toolbox of CDLD investigators, increase their competitiveness for funding, and enable them to advance as experts in digestive and liver disease research. The Core will also adopt a program of rigorous evaluation to ensuring optimal performance and continual access to new technologies as they are developed. The Cell Models Core represents a new core research resource at the Medical University of South Carolina. Currently, this technology is only available to a limited group of investigators and is temporarily run from the Duncan laboratory. Dr. Stephen A. Duncan, who will lead the core, is an expert in stem cell biology and cell differentiation, and he has used genome engineering extensively to generate cells to model liver disease. He has previous experience in running a similar core while a Professor at the Medical College of Wisconsin. The Core also recruited the expertise of Dr. Jorge Múnera who has pioneered the generation of colonic organoids from iPSCs. The Core leadership has already worked closely with the CDLD Junior Investigators to generate preliminary data for their proposed projects. Support through the COBRE will allow the Cell Models Core to expand its operation and provide these essential services to a broad community of CDLD investigators. A needs assessment survey ascertained that 21 established CDLD investigators and 3 junior investigators would use the core in the immediate future. The core will therefore have a substantial impact and advance digestive disease and liver research at MUSC.

细胞模型核心 - 项目摘要消化和肝病（CDLD）细胞模型核心的毛线将为CDLD研究者提供访问为消化和肝病的最新组织培养模型。我们将使用基因组工程包括CRIPSR-CAS9在内的技术，将特定的遗传改变引入已建立的细胞系中，然后进入诱导多能干细胞（IPSC）。 IPSC将被区分以形成胃肠道的细胞和CDLD研究人员将用于研究的肝脏。除了提供CDLD研究者细胞系以及可用于直接分析的类器官，细胞模型核心还将为研究人员配备指导，培训和关键试剂，使他们能够在自己的实验室中建立程序。通过培训机会，核心将推进CDLD调查人员的技术工具箱，增加他们对资金的竞争力，使他们能够成为消化和肝病研究专家的晋升。核心还将采用严格的评估计划，以确保最佳性能和持续访问开发新技术。细胞模型核心代表了一种新的核心研究资源南卡罗来纳州医科大学。目前，该技术仅适用于有限的一组调查人员暂时从邓肯实验室运行。 Stephen A. Duncan博士，他将领导核心是干细胞生物学和细胞分化方面的专家，他已经广泛使用了基因组工程产生细胞以建模肝病。他以前在运行类似核心方面经验威斯康星州医学院。核心还招募了豪尔赫·穆纳（JorgeMúnera）博士的专业知识 IPSCS的结肠器官产生。核心领导已经与CDLD紧密合作初级研究人员为其拟议项目生成初步数据。通过毛绒的支持将允许细胞模型核心扩展其运营并为广泛的社区提供这些基本服务 CDLD调查人员。需求评估调查确定，有21位既定的CDLD研究者和3个初级调查人员将在不久的将来使用核心。因此，核心将产生重大影响并在MUSC推进消化系统疾病和肝脏研究。