Reagentless Sensor Technologies For Continuous Monitoring of Heart Failure Biomarkers

用于连续监测心力衰竭生物标志物的无试剂传感器技术

基本信息

批准号：
10636089
负责人：
IGOR R EFIMOV
金额：
$ 76.97万
依托单位：
NORTHWESTERN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-20 至 2027-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10636089
关键词：
Acute Adhesions Adhesives Adopted Adult Affect American Anesthesia procedures Animal Model Benchmarking Biochemical Biological Biological Assay Biological Markers Biological Monitoring Blood Buffers Bulla Cardiac health Caregivers Cells Cellular Phone Charge Chronic Chronic Care Clinic Clinic Visits Clinical Complex Computer software Data Data Collection Deposition Dermis Detection Deterioration Development Devices Diagnosis Disease Disease Management Economics Electrocardiogram Electrodes Electronics Engineering Environmental Monitoring Enzyme-Linked Immunosorbent Assay Enzymes Exhibits FDA approved Filtration Gold Head Health Personnel Health Status Healthcare Heart Rate Heart failure Hospitalization Hospitals Hydrogels Hydrophobicity Implant In Situ In Vitro Individual Injections Intercellular Fluid Interleukin-1 beta Interleukin-6 Kinetics Measurement Membrane Metals Methods Modeling Molecular Monitor Natural regeneration Needles Nucleic Acids Operative Surgical Procedures Oxygen Patients Performance Peripheral Persons Pharmaceutical Preparations Phosphate Buffer Photoplethysmography Platinum Polymers Population Positioning Attribute Proteins Protocols documentation Pulmonary artery structure Pump Rattus Reagent Recurrence Reporter Reporting Risk Risk Assessment Running Saline Sampling Shapes Site Skin Specificity Stress Suction Surgical Models System TNF gene Technology Testing Textiles Thinness Time Titrations United States Validation Vertebral column Wistar Rats Work aorta constriction automated analysis biomarker identification biomarker panel biomaterial compatibility computer code design detection platform electrical measurement experience flexibility heart function hospital readmission hospitalization rates implantation implanted sensor in vivo in vivo monitoring instrumentation monitoring device monolayer nano novel strategies porous hydrogel preservation pressure prevent pro-brain natriuretic peptide (1-76)protein biomarkers prototype remote monitoring sensor sensor technology smartphone based device transmission process wearable device wearable monitor wearable platform wearable sensor technology wireless wireless transmission

项目摘要

Project Summary: Heart failure affects approximately twenty-six million people globally and represents a major economic and societal burden. This includes more than six million adults in the United States alone, a number that is predicted to rise by 46% over the next 15 years. Heart failure is a progressive condition characterized by recurrent exacerbations. Prior work indicates that biomarker monitoring can aid in the assessment of risk and provide meaningful data in management of the disease. But in order to allow this to be done on a continuous basis, self- regenerating sensors that can dynamically and continuously detect biomolecular species as a wearable system remain an unmet need. One major challenge to fully realize these systems is the development of platforms that allow for reagent-free analysis of complex biomolecules such as proteins, nucleic acids, and metabolites in real time. We recently developed the first reagentless sensor that can be adapted to any protein and demonstrated the ability to perform continuous monitoring in vivo using an implantable sensor array. In this project, we will adapt our technology for markers of heart failure to provide a new approach for remote monitoring of at-risk heart failure patients. The technology that enables this is a recently developed an enzyme-free and regent-free electrochemical reporter system that exhibits levels of sensitivity suitable for analysis of biological samples without the need for pre-processing. Electrochemical sensors are a powerful platform for environmental or biological monitoring based on inexpensive instrumentation and straightforward measurements of electrical currents. In the proposed project we will integrate these electrochemical sensors into biocompatible probes for in situ biochemical monitoring. We will test the sensors for sensitive and robust detection of a panel markers identified to be clinical indicators of cardiac health. Subsequently we will engineer these probes into a wearable platform capable of continuous monitoring of biomarkers present in the interstitial fluid. Requisite hardware and software for controlling the wearable monitoring device will be developed and tested in the project and automated analysis will be validated in an animal model of heart failure. The project deliverables will include first-in class biomarker monitoring systems with the potential to have a large impact on the American population by providing continual biomarker monitoring in healthcare for chronic conditions.

项目摘要：心力衰竭在全球大约影响2600万人，代表着主要的经济和社会负担。仅在美国就包括超过600万的成年人，这一数字可以预测在未来15年中增长46％。心力衰竭是一种渐进状态，以经常性为特征恶化。先前的工作表明，生物标志物监测可以帮助评估风险并提供疾病管理中有意义的数据。但是，为了允许连续完成此操作再生传感器，可以动态，连续地检测出生物分子物种作为可穿戴系统仍然是未满足的需求。充分意识到这些系统的一个主要挑战是平台的开发允许对复杂生物分子（例如蛋白质，核酸和代谢产物）进行无试剂分析。时间。我们最近开发了第一个可以适应任何蛋白质的无试剂传感器，并证明了能够使用可植入的传感器阵列在体内进行连续监测。在这个项目中，我们将适应我们为心力不断标记的技术提供了一种新的方法来远程监测高危心脏失败患者。实现这是最近开发的无酶的技术电化学报告基因系统，表现出适合分析生物样品的灵敏度水平无需预处理。电化学传感器是环境或基于廉价仪器和电气直接测量的生物监测电流。在拟议的项目中，我们将将这些电化学传感器整合到原位的生物相容性探针中生化监测。我们将测试传感器的敏感且可靠的检测已鉴定的面板标记成为心脏健康的临床指标。随后，我们将这些探针设计成可穿戴的平台能够连续监测间质液中存在的生物标志物。必要的硬件和软件用于控制可穿戴监控设备的项目和自动分析将开发和测试将在心力衰竭的动物模型中得到验证。该项目可交付成果将包括一流的生物标志物监视系统有可能通过提供持续的对美国人口产生重大影响医疗保健中的生物标志物监测慢性疾病。