Safeguarding Genetic Resources of Aquatic Biomedical Models

保护水生生物医学模型遗传资源

• 批准号: 10600565
• 负责人: Maria Teresa Gutierrez-Wing
• 金额: $ 17.76万
• 依托单位: LOUISIANA STATE UNIV AGRICULTURAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2024-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10600565
• 关键词: Address; Adult; Algae; Ambystoma; Amphibia; Animal Model; Animals; Aplysia; Aplysia species; Award; Basic Science; Biocompatible Materials; Biological; Biological Models; Collaborations; Communities; Community Developments; Consensus; Cryopreservation; Databases; Development; Documentation; Education and Outreach; Educational Curriculum; Educational process of instructing; Educational workshop; Embryo; Engineering; Ensure; Evolution; Fertilization; Foundations; Freezing; Funding; Genetic; Genetic Models; Genetic Variation; Growth; Human Resources; Individual; Information Systems; Internet; Invertebrates; Kentucky; Knowledge; Laboratories; Larva; Life; Link; Location; Maintenance; Methods; Microfluidics; Modeling; Monoclonal Antibody R24; Oocytes; Organism; Parents; Pathway interactions; Population; Postdoctoral Fellow; Process; Production; Productivity; Protocols documentation; Rana; Recommendation; Recovery; Reproducibility; Research; Research Infrastructure; Resources; Risk; Salamander; Sampling; Schedule; Secure; Services; Shipping; Somatic Cell; Source; Standardization; System; Technology; Time; Training; Training Programs; Transgenic Organisms; United States National Institutes of Health; Universities; Wood material; Work; Xenopus; Xiphophorus; Zebrafish; aquatic organism; base; biosecurity; coronavirus disease; cost; egg; experimental study; extreme weather; genetic information; genetic resource; improved; innovation; mature animal; mutant; next generation; novel strategies; outreach; outreach program; preservation; programs; protocol development; repository; sperm cell; stem cells; success

Project Summary/Abstract: Aquatic organisms are powerful biomedical models used in laboratories around the world. These animals are maintained mostly as live organisms, with the associated expense and risk. As thousands of new lines of mutants and transgenic organisms are generated, maintaining populations of each becomes prohibitive for stock centers. To address these problems, cryopreservation has become a necessity for the preservation of genetic resources. Cryopreservation must be linked to development of germplasm repositories where genetic resources are protected in a reproducible, standardized way. A well developed and maintained repository can reduce the cost of maintaining and recovering lines, reduce duplication of effort, and reduce the costs required to recreate lines. We are working to develop appropriate processes and repository technology for amphibians (i.e., Ambystoma and Xenopus) and invertebrates (i.e., Aplysia). Our current parent award is intended to provide access to community-level approaches for reproducibility, training, and standardization through a centralized resource Hub and a repository network that can address aquatic biomedical model repository needs. Through R24 funding the Hub is establishing capabilities to process germplasm samples and develop pathways for repository development. Currently, animals used for the project are sent from stock centers and other laboratories to the AGGRC. Our reliance on outside sources has become a substantial problem in ensuring the availability of adequate numbers of animals for replicated studies and outreach activities. Supplemental funds are requested herein to enable us to spawn and grow animals in-house. This will greatly advance the objectives of the parent award by increasing research productivity and outreach capability. Also, the risks associated with animal shipping, particularly in extreme weather conditions will be diminished. This work can be completed within the current project year (entering Year 3), and it does not overlap with work funded in the parent award or through previous supplemental funding. The specific use of this request is to provide in-house production live amphibians cultured from spawning through adulthood, to provide a timely source of broodstock to support the activities of the parent award in research, teaching, training, collaboration, and outreach.

项目摘要/摘要： 水生生物是世界各地实验室使用的强大生物医学模型。这些动物 主要作为活生物体进行维护，并具有相关的费用和风险。随着数以千计的新线路 突变体和转基因生物的产生，维持每种生物的种群变得令人望而却步 对于库存中心。为了解决这些问题，冷冻保存已成为必要 保护遗传资源。低温保存必须与种质的发育联系起来 以可重复、标准化的方式保护遗传资源的储存库。一个发展良好的 和维护的存储库可以降低维护和恢复线路的成本，减少重复 努力，并减少重新创建线路所需的成本。我们正在努力开发适当的流程 两栖动物（即钝口螈和爪蟾）和无脊椎动物（即海兔）的储存库技术。 我们目前的家长奖旨在为儿童提供社区层面的方法 通过集中资源中心和存储库网络实现可重复性、培训和标准化 可以满足水生生物医学模型库的需求。通过 R24 资助，该中心正在建立 处理种质样品和开发资源库开发途径的能力。现在， 用于该项目的动物从牲畜中心和其他实验室送到 AGGRC。我们的信赖 对外部资源的依赖已成为确保获得足够数量的资源的一个重大问题 用于重复研究和推广活动的动物。在此请求补充资金，以便能够 我们在内部繁殖和饲养动物。这将极大地推进家长奖的目标 提高研究生产力和推广能力。此外，与动物运输相关的风险， 特别是在极端天气条件下会减弱。这项工作可以在 当前项目年份（进入第 3 年），并且与家长奖励或资助的工作不重叠 通过之前的补充资金。此请求的具体用途是提供内部生产 从产卵到成年的活体两栖动物的培养，为它们提供及时的亲体来源 支持家长奖在研究、教学、培训、合作和推广方面的活动。