Safeguarding Genetic Resources of Aquatic Biomedical Models

保护水生生物医学模型遗传资源

• 批准号: 10600855
• 负责人: Maria Teresa Gutierrez-Wing
• 金额: $ 65.72万
• 依托单位: LOUISIANA STATE UNIV AGRICULTURAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10600855
• 关键词: Address; Algae; Ambystoma; Amphibia; Animals; Aplysia; Aplysia species; Basic Science; Biocompatible Materials; Biological; Biological Models; Biomedical Research; Communities; Community Developments; Consensus; Coupling; Cryopreservation; Database Management Systems; Development; Documentation; Educational Curriculum; Educational workshop; Embryo; Engineering; Ensure; Evolution; Failure; Fertilization; Freezing; Funding; Genetic; Genetic Models; Genetic Research; Genetic Transformation; Genetic Variation; Genotype; Germ; Human Resources; Individual; Information Systems; Internet; Invertebrates; Investments; Kentucky; Knowledge; Laboratories; Laboratory Research; Larva; Life; Link; Location; Maintenance; Methods; Microfluidics; Modeling; Network-based; Oocytes; Organism; Pathway interactions; Process; Protocols documentation; Quality Control; Rana; Recommendation; Recovery; Reproducibility; Research; Research Infrastructure; Resources; Risk; Salamander; Sampling; Secure; Services; Somatic Cell; Standardization; System; Techniques; Technology; Time; Training; Training Programs; Transgenic Organisms; Translating; United States National Institutes of Health; Universities; Work; Xenopus; Xiphophorus; Zebrafish; aquatic organism; cost; cost shifting; egg; genetic information; genetic resource; human disease; improved; innovation; marine; model organism; mutant; neglect; next generation; novel strategies; outreach program; preservation; prevent; programs; protocol development; repository; sperm cell; stem cells; success; wasting

PROJECT SUMMARY/ABSTRACT: The genetic resources of aquatic biomedical organisms are the products of millions of years of evolution, decades of scientific development, and hundreds of millions of dollars of research funding investment. Aquatic organisms have become powerful models in biomedical research and laboratories around the world have produced tens of thousands of mutant and transgenic lines. Maintaining these valuable genotypes as live animals is expensive, risky, and beyond the capacity of most stock centers. As such, cryopreservation has become a necessity and typically these genetic resources are now maintained as samples of inconsistent quality frozen with rudimentary techniques. Quality control has not been practiced in any systematic way, reproducibility is poor, and protocols are not standardized. It is common to have problems and failures in fertilization resulting in lost lines that need to be recreated, causing facilities to waste considerable time and effort. This is largely due to the false notion that neglecting quality control saves time and money. However, rather than being reduced, these costs are shifted downstream through wasted storage space and reduced fertilization. This pervasive lack of reliability and reproducibility has placed the substantial investments in biomedical research at great risk. These pervasive problems were the subject of a 2017 NIH Cryopreservation Workshop to develop germplasm repositories to protect aquatic biomedical genetic resources. This proposal directly responds to the specific needs identified by research communities and the Directors of the five NIH-funded aquatic animal stock centers at that workshop. The mechanism identified to deliver the much-needed research and capacity development was through establishment of a Hub and repository network based on the model of the AGGRC, which is specifically directed at translating research into applied practice. The Specific Aims of this proposal are to: 1) Establish comprehensive repository systems at NIH-funded stock centers for sperm of Xenopus frogs and Ambystoma salamanders. 2) Establish a comprehensive repository system at the NIH-funded stock center for early life stages (e.g., embryos, larvae) of Aplysia sea hares and required algae species. 3) Establish a comprehensive, centralized unit (“Hub”) to integrate activities across NIH-funded stock centers and their communities, and develop approaches and documentation for cryopreservation. This includes development of protocols and pathways, outreach programs, community interaction, standardization, freezing services, and training. The AGGRC was developed to directly address these needs and is uniquely suited to perform and integrate the necessary research, stock center, and network-level activities.

项目摘要/摘要： 水生生物医学生物的遗传资源是数百万年的产物 进化，几十年的科学发展和数亿美元的研究 资金投资。水生生物已成为生物医学研究的强大模型 世界各地的实验室产生了成千上万的突变体和转基因 线。将这些有价值的基因型保持为活动物是昂贵，冒险的，超出了 大多数股票中心的容量。因此，冷冻保存已成为必要的 这些遗传资源现在被保存为冻结质量不一致的样本 基本技术。质量控制尚未以任何系统的方式进行， 可重复性差，协议不标准化。有问题和 受精的失败导致需要重新创建的丢失线，导致设施浪费 大量的时间和精力。这主要是由于忽略质量控制的错误观念 节省时间和金钱。但是，这些成本并没有减少，而是改变了 通过浪费的存储空间下游并减少受精。这种普遍的缺乏 可靠性和可重复性使生物医学研究的大量投资 巨大的风险。这些普遍的问题是2017年NIH冷冻保存的主题 开发种质存储库以保护水生生物医学遗传资源的研讨会。 该提案直接响应研究社区确定的特定需求和 该研讨会上五个由NIH资助的水上动物库存中心的董事。机制 确定提供急需的研究和能力发展是通过 基于AGGRC的模型建立集线器和存储库网络， 专门用于将研究转化为应用实践。这个特定的目的 提案是：1）在NIH资助的股票中心建立全面的存储库系统 爪蟾青蛙和Ambystoma salamanders的精子。 2）建立一个全面的存储库 NIH资助的股票中心的系统早期阶段（例如，胚胎，幼虫）的系统 野兔和必需的藻类物种。 3）建立一个全面的集中式单位（“集线器”） NIH资助的股票中心及其社区的整合活动，并发展 冷冻保存的方法和文档。这包括协议的制定 以及途径，外展计划，社区互动，标准化，冻结服务， 和培训。 AGGRC的开发是为了直接满足这些需求，并且非常适合 执行和集成必要的研究，股票中心和网络级活动。