Safeguarding Genetic Resources of Aquatic Biomedical Models

保护水生生物医学模型遗传资源

基本信息

批准号：
10450940
负责人：
Maria Teresa Gutierrez-Wing
金额：
$ 27.07万
依托单位：
LOUISIANA STATE UNIV AGRICULTURAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-06-15 至 2024-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10450940
关键词：
Address Algae Ambystoma Amphibia Animal Model Animals Aplysia Aplysia species Basic Science Biocompatible Materials Biological Biological Models Biomedical Research Communities Community Developments Consensus Coupling Cryopreservation Databases Development Documentation Educational Curriculum Educational workshop Embryo Engineering Ensure Evolution Failure Fertilization Foundations Freezing Funding Genetic Genetic Models Genetic Research Genetic Variation Genotype Human Resources Individual Information Systems Internet Invertebrates Investments Kentucky Knowledge Laboratories Laboratory Research Larva Life Link Location Maintenance Methods Microfluidics Modeling Network-based Oocytes Organism Pathway interactions Postdoctoral Fellow Process Protocols documentation Quality Control Rana Recommendation Recovery Reproducibility Research Research Infrastructure Resources Risk Salamander Sampling Secure Services Somatic Cell Standardization System Techniques Technology Time Training Training Programs Transgenic Organisms Translating United States National Institutes of Health Universities Wood material Work Xenopus Xiphophorus Zebrafish aquatic organism base cost cost shifting egg genetic information genetic resource human disease improved innovation mutant neglect next generation novel strategies outreach program preservation prevent programs protocol development repository sperm cell stem cells success wasting

项目摘要

PROJECT SUMMARY/ABSTRACT: The genetic resources of aquatic biomedical organisms are the products of millions of years of evolution, decades of scientific development, and hundreds of millions of dollars of research funding investment. Aquatic organisms have become powerful models in biomedical research and laboratories around the world have produced tens of thousands of mutant and transgenic lines. Maintaining these valuable genotypes as live animals is expensive, risky, and beyond the capacity of most stock centers. As such, cryopreservation has become a necessity and typically these genetic resources are now maintained as samples of inconsistent quality frozen with rudimentary techniques. Quality control has not been practiced in any systematic way, reproducibility is poor, and protocols are not standardized. It is common to have problems and failures in fertilization resulting in lost lines that need to be recreated, causing facilities to waste considerable time and effort. This is largely due to the false notion that neglecting quality control saves time and money. However, rather than being reduced, these costs are shifted downstream through wasted storage space and reduced fertilization. This pervasive lack of reliability and reproducibility has placed the substantial investments in biomedical research at great risk. These pervasive problems were the subject of a 2017 NIH Cryopreservation Workshop to develop germplasm repositories to protect aquatic biomedical genetic resources. This proposal directly responds to the specific needs identified by research communities and the Directors of the five NIH-funded aquatic animal stock centers at that workshop. The mechanism identified to deliver the much-needed research and capacity development was through establishment of a Hub and repository network based on the model of the AGGRC, which is specifically directed at translating research into applied practice. The Specific Aims of this proposal are to: 1) Establish comprehensive repository systems at NIH-funded stock centers for sperm of Xenopus frogs and Ambystoma salamanders. 2) Establish a comprehensive repository system at the NIH-funded stock center for early life stages (e.g., embryos, larvae) of Aplysia sea hares and required algae species. 3) Establish a comprehensive, centralized unit (“Hub”) to integrate activities across NIH-funded stock centers and their communities, and develop approaches and documentation for cryopreservation. This includes development of protocols and pathways, outreach programs, community interaction, standardization, freezing services, and training. The AGGRC was developed to directly address these needs and is uniquely suited to perform and integrate the necessary research, stock center, and network-level activities.

项目摘要/摘要：水生生物医学生物遗传资源是亿万年的产物进化、数十年的科学发展和数亿美元的研究资金投资。水生生物已成为生物医学研究的强大模型。世界各地的实验室已经生产了数以万计的突变体和转基因体维持这些有价值的基因型作为活体动物是昂贵的、有风险的，而且超出了我们的承受范围。因此，冷冻保存已成为一种必要且通常的做法。这些遗传资源现在作为质量不一致的样本进行保存，并冷冻尚未以任何系统的方式实施基本的质量控制，重现性差，实验方案不标准化，经常出现问题。施肥失败导致需要重新创建的品系丢失，导致设施浪费这很大程度上是由于忽视质量控制的错误观念。节省时间和金钱。然而，这些成本并没有减少，而是转移了。下游由于储存空间浪费和施肥减少而普遍存在。可靠性和可重复性使生物医学研究的大量投资这些普遍存在的问题是 2017 年 NIH 冷冻保存的主题。开发种质资源库以保护水生生物医学遗传资源研讨会。该提案响应了研究界和学术界直接确定的具体需求 NIH 资助的五个水生动物饲养中心的主任参加了该研讨会。确定提供急需的研究和能力发展是通过基于AGGRC模型建立Hub和repository网络，即专门针对将研究转化为应用实践。建议： 1) 在 NIH 资助的库存中心建立综合存储系统爪蟾和钝口蝾螈的精子 2) 建立综合储存库。美国国立卫生研究院 (NIH) 资助的海兔早期生命阶段（例如胚胎、幼虫）储备中心的系统野兔和所需的藻类种类 3) 建立一个综合性的集中单位（“中心”）来整合 NIH 资助的库存中心及其社区的活动，并制定冷冻保存的方法和文档这包括协议的制定。和途径、外展计划、社区互动、标准化、冷冻服务、 AGGRC 的开发是为了直接满足这些需求，并且非常适合。执行和整合必要的研究、库存中心和网络级活动。