Microcyclone arrays for high resolution bioaerosol fractionation and viable virus collection

用于高分辨率生物气溶胶分级和活病毒收集的微旋风阵列

• 批准号: 10593436
• 负责人: Don L DeVoe
• 金额: $ 19.43万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-12-22 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10593436
• 关键词: 3-Dimensional; 3D Print; Address; Aerosols; Air; Alveolus; Bacteria; Biological Availability; Breathing; Bypass; Clinical Research; Collection; Complex; Coughing; Coupled; Development; Devices; Diameter; Dimensions; Disease; Disease model; Elements; Environment; Evaluation; Exhalation; Exhibits; Fractionation; Fungal Spores; Future; Goals; Hour; Hybrids; Hydration status; Hydrogels; Immobilization; Individual; Infection; Influenza; Inhalation; Laboratories; Lung; Microspheres; Modeling; Molds; Mucous body substance; Particle Size; Pattern; Penetration; Performance; Persons; Plant Resins; Plaque Assay; Polystyrenes; Process; Quantitative Reverse Transcriptase PCR; Resolution; Respiratory Disease; Respiratory Process; Risk; Sampling; Series; Sneezing; Stream; Structure; System; Techniques; Technology; Validation; Viral; Virus; aerosolized; design; disease transmission; fabrication; improved; influenza infection; influenzavirus; innovation; instrument; lithography; manufacture; meter; operation; particle; pathogen; residence; respiratory virus; sample collection; technology validation; tool; transmission process; viral transmission

PROJECT SUMMARY Our objective is to develop a multi-stage microscale cyclone technology that will serve as an efficient and scalable platform for bioaerosol fractionation, enabling more effective studies of fundamental and applied viral aerobiology. The microcyclones will be designed to isolate selected aerosol size fractions collected from exhaled breath samples, enabling the distribution of respiratory virus within these samples to be evaluated with high size resolution. Significantly, the technology will be designed to overcome the limitations of existing aerobiological instruments by enhancing the dynamic size range, maximum number of collected fractions, resolution, throughput, and bioefficiency. The platform will take advantage of a high resolution stereolithography-based 3d printing technique to pattern large arrays of complex microcyclone structures in a monolithic substrate, with multiple arrays placed in series to allow selected aerosol size ranges to be isolated from the sample flow. The system will further support the integration of a hydrogel layer within each microcyclone array to allow the capture of live virus for infectivity studies. The microcyclone arrays will be combined with an established exhaled breath collection system and employed to study the distribution of influenza virus from infected subjects. To support these goals, individual microcyclone elements will be designed, fabricated, characterized, and optimized for isolating at least five distinct aerosol size fractions ranging from 200 nm to 10 µm, followed by the development of full microcyclone arrays integrating hundreds of individual separation elements in a single device. A set of cascaded arrays will be assembled into a reusable cartridge to enable collection of all target fractions within a single integrated unit, and the resulting cartridge will be interfaced with the existing system for high- volume exhaled breath collection (the G-II sampler developed in the Milton laboratory). The integrated instrument will be used to collect exhaled breath samples from at least 15 individuals with active influenza infection for the evaluation of virus distribution across all size fractions. The study results are expected to validate the technology as a powerful tool for enhancing our understanding of aerobiology and improving modeling, risk analysis, and mitigation strategies for a wide range of airborne diseases. Successful completion of these aims will offer a clear view of the potential of the microcyclone array technology for collection and high resolution fractionation of bioaerosols from exhaled breath samples. For future steps we envision optimizing bioefficiency of the technology to support culture of collected virus, scaling the arrays to support environmental sampling at higher flow rates, and extending application of the technology to the characterization of aerosolized bacteria and fungal spores.

项目摘要 我们的目标是开发一种多阶段的微观旋风技术技术，该技术将作为一种有效且可扩展的 生物溶质分馏的平台，可以对基本和应用病毒生物学的更有效研究。这 微气囊将设计为隔离从耗尽的呼吸样品中收集的选定的气溶胶尺寸分数， 可以通过高尺寸分辨率评估这些样品中呼吸道病毒的分布。显著地， 该技术将旨在克服现有的特技生物学工具的局限性。 动态尺寸范围，收集的分数的最大数量，分辨率，吞吐量和生物效率。平台 将利用基于高分辨率的基于立体光刻的3D打印技术来模板 整体底物中的复杂的微孔子结构，串联放置多个阵列以允许选定 气溶胶大小范围从样品流中分离出来。该系统将进一步支持水凝胶的整合 每个微核阵列中的一层，以允许捕获活病毒进行感染研究。微孔克隆阵列 将与已建立疲惫的呼吸收集系统结合在一起，并用于研究 感染受试者的流感病毒。为了支持这些目标，将设计单个的微孔元素元素 制造，表征和优化，用于隔离至少五个不同的气溶胶尺寸，范围从200 nm到 10 µm，然后开发全面的微孔阵列，集成了数百个单独的分离元件 在一个设备中。一组级联的阵列将组装成可重复使用的墨盒，以使所有目标收集 单个集成单元中的分数，由此产生的墨盒将与现有系统连接用于高级系统 呼吸量耗尽（在米尔顿实验室开发的G-II采样器）。综合仪器将 用于收集至少有15个活跃影响力感染的人的裸露呼吸样本进行评估 所有大小分数的病毒分布。预计研究结果将验证该技术是一种强大的 增强我们对运动生物学并改善建模，风险分析和缓解策略的工具 用于广泛的空中疾病。这些目标的成功完成将清楚地了解 微孔阵列技术，用于收集和高分辨率的生物溶质分馏 样品。对于将来的步骤，我们设想优化技术的生物效率以支持收集的病毒的文化， 扩展阵列以在较高的流速下支持环境采样，并扩展技术的应用 为了表征雾化细菌和真菌孢子。