Microcyclone arrays for high resolution bioaerosol fractionation and viable virus collection

用于高分辨率生物气溶胶分级和活病毒收集的微旋风阵列

基本信息

批准号：
10593436
负责人：
Don L DeVoe
金额：
$ 19.43万
依托单位：
UNIV OF MARYLAND, COLLEGE PARK
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-12-22 至 2024-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10593436
关键词：
3-Dimensional 3D Print Address Aerosols Air Alveolus Bacteria Biological Availability Breathing Bypass Clinical Research Collection Complex Coughing Coupled Development Devices Diameter Dimensions Disease Disease model Elements Environment Evaluation Exhalation Exhibits Fractionation Fungal Spores Future Goals Hour Hybrids Hydration status Hydrogels Immobilization Individual Infection Influenza Inhalation Laboratories Lung Microspheres Modeling Molds Mucous body substance Particle Size Pattern Penetration Performance Persons Plant Resins Plaque Assay Polystyrenes Process Quantitative Reverse Transcriptase PCR Resolution Respiratory Disease Respiratory Process Risk Sampling Series Sneezing Stream Structure System Techniques Technology Validation Viral Virus aerosolized design disease transmission fabrication improved influenza infection influenzavirus innovation instrument lithography manufacture meter operation particle pathogen residence respiratory virus sample collection technology validation tool transmission process viral transmission

项目摘要

PROJECT SUMMARY Our objective is to develop a multi-stage microscale cyclone technology that will serve as an efficient and scalable platform for bioaerosol fractionation, enabling more effective studies of fundamental and applied viral aerobiology. The microcyclones will be designed to isolate selected aerosol size fractions collected from exhaled breath samples, enabling the distribution of respiratory virus within these samples to be evaluated with high size resolution. Significantly, the technology will be designed to overcome the limitations of existing aerobiological instruments by enhancing the dynamic size range, maximum number of collected fractions, resolution, throughput, and bioefficiency. The platform will take advantage of a high resolution stereolithography-based 3d printing technique to pattern large arrays of complex microcyclone structures in a monolithic substrate, with multiple arrays placed in series to allow selected aerosol size ranges to be isolated from the sample flow. The system will further support the integration of a hydrogel layer within each microcyclone array to allow the capture of live virus for infectivity studies. The microcyclone arrays will be combined with an established exhaled breath collection system and employed to study the distribution of influenza virus from infected subjects. To support these goals, individual microcyclone elements will be designed, fabricated, characterized, and optimized for isolating at least five distinct aerosol size fractions ranging from 200 nm to 10 µm, followed by the development of full microcyclone arrays integrating hundreds of individual separation elements in a single device. A set of cascaded arrays will be assembled into a reusable cartridge to enable collection of all target fractions within a single integrated unit, and the resulting cartridge will be interfaced with the existing system for high- volume exhaled breath collection (the G-II sampler developed in the Milton laboratory). The integrated instrument will be used to collect exhaled breath samples from at least 15 individuals with active influenza infection for the evaluation of virus distribution across all size fractions. The study results are expected to validate the technology as a powerful tool for enhancing our understanding of aerobiology and improving modeling, risk analysis, and mitigation strategies for a wide range of airborne diseases. Successful completion of these aims will offer a clear view of the potential of the microcyclone array technology for collection and high resolution fractionation of bioaerosols from exhaled breath samples. For future steps we envision optimizing bioefficiency of the technology to support culture of collected virus, scaling the arrays to support environmental sampling at higher flow rates, and extending application of the technology to the characterization of aerosolized bacteria and fungal spores.

项目概要我们的目标是开发一种多级微型旋风分离器技术，该技术将作为一种高效且可扩展的技术生物气溶胶分级分离平台，能够更有效地研究基础和应用病毒气生物学。微旋风分离器将被设计用于分离从呼出气体样本中收集的选定气溶胶尺寸部分，能够以高分辨率评估这些样本中呼吸道病毒的分布。该技术旨在通过增强动态尺寸范围、收集的级分的最大数量、分辨率、通量和生物效率。将利用基于高分辨率立体光刻的 3D 打印技术来图案化大型阵列单片基板中的复杂微旋风器结构，多个阵列串联放置以允许选择该系统将进一步支持水凝胶的集成。每个微旋风分离器阵列内都有一层层，可以捕获活病毒以进行感染性研究。将与已建立的呼气收集系统相结合，用于研究呼气的分布为了支持这些目标，将设计单独的微旋风元件，制造、表征和优化用于分离至少五种不同的气溶胶尺寸分数，范围从 200 nm 到 10 µm，随后开发了集成数百个独立分离元件的完整微旋风分离器阵列在单个设备中，一组级联阵列将被组装到可重复使用的盒中，以收集所有目标。单个集成单元内的分数，所得的墨盒将与现有系统连接以实现高呼出气量采集（Milton 实验室开发的 G-II 采样器）。用于收集至少 15 名活动性流感感染者的呼出气样本进行评估研究结果预计将验证该技术的强大功能。增强我们对空气生物学的理解并改进建模、风险分析和缓解策略的工具成功完成这些目标将使人们清楚地了解该技术的潜力。用于收集和高分辨率分离呼出气体中的生物气溶胶的微旋风阵列技术对于未来的步骤，我们设想优化该技术的生物效率以支持收集的病毒的培养，扩展阵列以支持更高流速的环境采样，并扩展该技术的应用雾化细菌和真菌孢子的表征。