Advanced Bioaerosols Technology Core

先进的生物气溶胶技术核心

• 批准号: 10471984
• 负责人: Don L DeVoe
• 金额: $ 70.03万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10471984
• 关键词: Address; Aerosols; Biocompatible Materials; Biological Assay; Biomedical Engineering; Cell Culture Techniques; Cell Line; Clinical; Clinical Research; Collection; Controlled Environment; Coupled; Development; Devices; Engineering; Environment; Evaluation; Exhalation; Exhibits; Ferrets; Fractionation; Gelatin; Goals; Growth; Heterogeneity; Human; Hydrogels; Individual; Influenza; Investigation; Liquid substance; Lung; Methods; Microfluidics; Modeling; Monitor; Mucins; Mucous body substance; Particle Size; Physical condensation; Population; Property; Research; Research Project Grants; Resolution; Respiratory System; Reverse Transcriptase Polymerase Chain Reaction; Risk; Risk Assessment; Route; Sampling; Solid; Source; Support System; Surface; System; Techniques; Technology; Variant; Viral; Virion; Virus; Water; ambient particle; base; density; design; digital; flu transmission; improved; influenzavirus; innovation; innovative technologies; instrument; model development; novel; pandemic disease; particle; prevent; respiratory; sample collection; tool; transmission process; virology

Project Summary/Abstract – Advanced Bioaerosol Technology Core The Advanced Bioaerosol Technology Core (ABTC) will develop a comprehensive suite of innovative technologies for improved sampling, fractionation, culture, and characterization of influenza virus aerosols to enable new analytical capabilities in support of the U19 research project goals. The advanced technologies developed through the ABTC will be employed within each individual research project to yield more effective studies of airborne transmission and infectivity through a combination of efficient ambient sampling to evaluate size-dependent distributions of infective virus within the clinical environment, and high-resolution individual exhaled breath sampling to elucidate the fundamental source terms needed for accurate transmission modeling. The ABTC team will leverage expertise across the fields of bioengineering, bioaerosol system engineering, and respiratory virology to develop 5 interconnected technologies for advanced bioaerosol analysis. In Aim 1, a set of compact instruments will be developed for the collection and parallel size-based fractionation of ambient environmental aerosols to enable distributed monitoring of airborne virus during the proposed clinical studies. In Aim 2, a second aerosol sampling instrument employing a unique exhaled breath sampling technology will be developed to provide high resolution aerosol fractionation and collection, allowing us to evaluate both viable and non-infective virus emission from individual donors across narrow particle size ranges. To enhance infectivity characterization for sampled virus particles, a synthetic mucus hydrogel will be advanced in Aim 3 as a novel sample target for the environmental sampler, providing improved virus capture and cell culture, greatly improving virus capture and viability for downstream infectivity assays. A digital cell culture array technology will be developed in Aim 4, enabling the direct isolation of individual aerosol particles captured by the exhaled breath sampler, and allowing us to evaluate distribution and clustering of both viable and non-infective virus units at single particle resolution. The ABTC team will also develop an optimized cell line that will be employed for the analysis of virus collected with the developed instruments to enhance evaluation of infectivity. The core will validate the developed technologies for aerosol sampling, capture, isolation, culture, and analysis through a set of ferret studies before integration with the proposed U19 research projects, and will execute the required assays using clinical samples to support the goals of RP1 and RP2. The results of the ABTC research efforts will yield the instruments and technologies needed to enhance our understanding of influenza aerobiology, from the determination of critical source terms to the evaluation of infective particle distributions within the environment, supporting significant improvements to influenza transmission modeling, risk analysis, and mitigation, while making important contributions toward advancing our understanding of other respiratory viral pandemic threats.

项目摘要/摘要 - 先进的生物Aerosol技术核心 先进的生物Aerosol技术核心（ABTC）将开发全面的创新套件 改进的技术，分馏，培养和表征有影响力病毒气溶胶的技术 启用新的分析能力以支持U19研究项目目标。高级技术 通过ABTC开发的每个研究项目都将采用，以产生更有效的 通过有效的环境采样组合进行空中传播和感染的研究以评估 临床环境中感染性病毒的大小依赖性分布和高分辨率个体 呼出的呼吸采样，以阐明准确的传输建模所需的基本源术语。 ABTC团队将利用生物工程，生物Aerosol系统工程和 呼吸道病毒学开发了5种互连技术，用于晚期生物溶质分析。在AIM 1中，一套 将开发紧凑型仪器的收集和基于平行尺寸的环境分级 在拟议的临床研究中，环境气溶胶能够对空气中病毒进行分布的监测。在 AIM 2，使用独特的呼气呼吸抽样技术的第二台气溶胶采样工具将是 开发的旨在提供高分辨率的气溶胶分级和收集，使我们能够评估可行和 狭窄粒径范围内的个别供体的非感染病毒发射。增强感染 对采样病毒颗粒的表征，合成粘液水凝胶将在AIM 3中作为新颖 环境采样器的样本目标，提供改善的病毒捕获和细胞培养，大大改善 病毒捕获和下游感染分析的生存能力。数字细胞文化阵列技术将是 在AIM 4中开发，使能够直接隔离疲惫的呼吸捕获的单个气溶胶颗粒 采样器，允许我们评估可在 单粒子分辨率。 ABTC团队还将开发一条优化的单元线，将为 用开发仪器收集的病毒分析以增强感染评估。核心意愿 通过一组验证开发的气溶胶采样，捕获，隔离，培养和分析的技术 在与拟议的U19研究项目集成之前，将进行雪貂研究，并将执行所需的测定法 使用临床样本来支持RP1和RP2的目标。 ABTC研究工作的结果将产生 从 确定对环境内感染粒子分布评估的关键源术语， 支持对影响力传播建模，风险分析和缓解措施的重大改进，而 为促进我们对其他呼吸道病毒大流行威胁的理解做出重要贡献。