Core C: Genomics and High Throughput Screening Core

核心 C：基因组学和高通量筛选核心

• 批准号: 8066104
• 负责人: SCOTT L DIAMOND
• 金额: $ 25.95万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8066104
• 关键词: Core; Genomics; Throughput; Screening

This Core provides cutting edge tools that allow invesfigators to monitor cellular and molecular changes that occur during normal hematopoiesis or in pathological states associated with bone marrow failure (BMF), nonmalignant myelodysplastic syndromes or defects in erythropoiesis/megakaryopoiesis, addressing the three foci of this application as detailed in the OVERALL DESCRIPTION. There is a clear need for services to identify and analyze in a rapid, high throughput fashion drugs that influence hematopoiefic cell commitment to various lineages and to develop miniaturized systems for studies of stromal/niche environments. Dr. Diamond is the director of the University of Pennsylvania (UPENN) PCMD, which he established as a Core Facility in 2005 (see BIOSKETCH). Dr. Diamond has 20 years of experience with numerous projects in endothelial biology, blood biology, and blood systems biology. He already has been working with Drs. Poncz, French and Gadue on niche effects on thrombopoiesis from mature megakaryocytes. He will be overall Director and Director of SubCore C-1. Dr. Baldwin will direct the analysis of RNA and DNA changes in cells during hematopoiesis. He has 11 years of industrial and academic experience in microarray assays, serves on the Neuroscience Microarray Consortium Advisory Panel for the NIH, co-chairs the MicroArray Research Group of the Associafion of Biomolecular Resource Facilifies, and interacts regularly with core directors on a nafional level (see BIOSKETCH). Dr. Baldwin founded the Penn Microarray Facility in 2001, was appointed Director of the Molecular Diagnosis and Genotyping Facility in 2005, and has merged the two laboratories to create the Molecular Profiling Facility. He has conducted previous genomic profiling projects with Drs. Weiss, Blobel, Carroll, Diamond, Discher, Gewirtz, June and Pear on efforts directly relevant to this P30. We have combined components of the two UPENN cores run by Drs. Diamond and Baldwin to develop a single core for this P30 to incorporate state-of-the-art resources on our campus that will unite our abilities to analyze normal, pathologic and manipulated hematopoietic cells. SubCore C-1 offers high-throughput microscale screening for compounds that affect various aspects of hematopoiefic cell biology, including lineage commitment (either from embryonic stem (ES) cells or more differentiated progenitors), survival, proliferation or maturafion into mature blood cells. SubCore C-2 will analyze changes that occur in the RNA expression profiles and epigenetic DNA marks in these cells either de novo or after modifications using Core E. We believe that these two SubCores reflect a common theme of rapidly analyzing cells and cellular changes using complex methodologies with significant investment in rapidly changing equipment and technologies. Core C is committed to bringing the most current and innovative technologies to the P30 investigators, while providing campus-wide standardization of services to support cooperafive efforts by different invesfigators with complementary interests and expertise. Our goal is to provide significant labor-intensive service and guidance at very compefitive pricing, supported in part by efficiency of utilizafion and by UPENN financial support.

该核心提供了尖端工具，使研究人员能够监测正常造血过程中或与骨髓衰竭 (BMF)、非恶性骨髓增生异常综合征或红细胞生成/巨核细胞生成缺陷相关的病理状态下发生的细胞和分子变化，解决了该应用的三个焦点如总体描述中详述。显然需要服务来识别和 以快速、高通量的方式分析影响造血细胞对各种谱系的承诺的药物，并开发用于基质/生态位环境研究的小型化系统。 Diamond 博士是宾夕法尼亚大学 (UPENN) PCMD 的主任，该中心是他于 2005 年建立的核心设施（请参阅 BIOSKETCH）。 Diamond 博士拥有 20 年在内皮生物学、血液等领域的众多项目经验 生物学和血液系统生物学。他已经和博士一起工作了。 Poncz、French 和 Gadue 探讨了成熟巨核细胞血小板生成的生态位效应。他将担任 SubCore C-1 的总体总监和总监。 Baldwin 博士将指导分析造血过程中细胞中 RNA 和 DNA 的变化。他在微阵列检测方面拥有 11 年的行业和学术经验，在 NIH 神经科学微阵列联盟顾问小组中任职，担任生物分子资源促进协会微阵列研究小组的联合主席，并定期与国家层面的核心董事进行互动 （参见 BIOSKETCH）。 Baldwin 博士于 2001 年创立了宾夕法尼亚大学微阵列设施，于 2005 年被任命为分子诊断和基因分型设施主任，并将两个实验室合并创建了分子分析设施。他之前曾与博士一起进行过基因组分析项目。 Weiss、Blobel、Carroll、Diamond、Discher、Gewirtz、June 和 Pear 讨论了与本 P30 直接相关的工作。 我们结合了 Drs 运行的两个 UPENN 核心的组件。 Diamond 和 Baldwin 将为 P30 开发一个单一核心，以整合我们校园中最先进的资源，这将结合我们分析正常、病理和操纵造血细胞的能力。 SubCore C-1 可对影响造血细胞生物学各个方面的化合物进行高通量微量筛选，包括谱系定型（来自胚胎干 (ES) 细胞或分化程度更高的祖细胞）、存活、增殖或成熟为成熟血细胞。 SubCore C-2 将分析这些细胞中 RNA 表达谱和表观遗传 DNA 标记发生的变化，无论是从头开始还是使用 Core E 修改后。我们相信这两个 SubCore 反映了使用复杂的快速分析细胞和细胞变化的共同主题对快速变化的设备和技术进行大量投资的方法。 Core C 致力于为 P30 研究人员带来最新和创新的技术，同时提供校园范围内的标准化服务，以支持具有互补兴趣和专业知识的不同研究人员的合作努力。我们的目标是以极具竞争力的价格提供重要的劳动密集型服务和指导，部分支持是利用效率和 UPENN 财务支持。