Next Generation Sequencing Core

下一代测序核心

• 批准号: 10714179
• 负责人: ERLE S. ROBERTSON
• 金额: $ 45.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2028-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10714179
• 关键词: ATAC-seq; Address; Award; Bioinformatics; Biological Assay; Biometry; Cancer Center; Center Core Grants; ChIP-seq; Complement; Complex; Core Facility; DNA Sequencing Facility; DNA sequencing; Data; Data Analyses; Genetic Transcription; Genomics; Goals; Herpesviridae; High-Throughput Nucleotide Sequencing; Human Genome; Human Herpesvirus 4; Human Herpesvirus 8; Hypoxia; Leadership; Libraries; Maps; Methodology; Methods; Oncogenic; Oncogenic Viruses; Online Systems; Preparation; Procedures; Protocols documentation; Research Personnel; Sampling; Services; Standardization; Technical Expertise; Technology; Work; chromatin immunoprecipitation; data management; experience; experimental study; follow-up; medical schools; member; next generation sequencing; programs; success; transcriptome sequencing

Summary Core C: Next Generation Sequencing core (NGSC) The next generation sequencing core (NGSC) will be responsible for all next generation sequencing activities by the four projects. All sequencing activities will be performed in Core C. RNA-Seq, ChIP-Seq, ATAC-Seq, Core C will be responsible for 1) performing NGS-assays; 2) generating library for Illumina next-generation sequencing, 3) mapping sequence data to EBV, KSHV, MPyV and human genomes, and 4) providing all follow-up bioinformatics and biostatistics analyses for the overall program. These goals will be accomplished in conjunction with the Penn Genomics, Next-Gen-Sequencing and Bioinformatics Cores, which provides Illumina Next Generation Sequencing as a core service. Final data management and analyses will be made accessible to each project investigator via a password protected shared web-based browser for program investigators. Expertise in all aspects of Next-Gen-Sequencing (NGS), Chromatin immunoprecipitation, and Bioinformatics support will be provided to the projects for the lifetime of the award. The NGS core will be led by experience leaders in administration and technical expertise to ensure that the core will be successful. Dr. Lieberman, is an expert in NGS and is familiar with all aspects of NGS technologies. Dr. Robertson has led many large complex programs, and is familiar with management and coordination of the technical expertise needed for success. Thus, the leadership is familiar with different NGS methodologies and bioinformatics analyses as applied to oncogenic herpesviruses and the human genome. Core C leaders have both provide unique expertise that complement each other. The NGS Core C will facilitate the ability of all members of the Program to standardize methods for sample preparation and for post-sequence bioinformatics analyses. The work will be performed at the Perelman School of Medicine in space adjacent to the Robertson lab, and in conjunction with the Penn Genomics NGS Core, and Bioinformatics core supported in part by the Abramson Cancer Center.

概括 核心 C：下一代测序核心 (NGSC) 下一代测序核心（NGSC）将负责所有下一代测序活动 由四个项目。所有测序活动都将在 Core C 中进行。RNA-Seq、ChIP-Seq、ATAC-Seq、 核心 C 将负责 1) 进行 NGS 检测； 2) 为Illumina下一代生成库 测序，3) 将序列数据映射到 EBV、KSHV、MPyV 和人类基因组，以及 4) 提供所有 整个计划的后续生物信息学和生物统计学分析。这些目标将在 与 Penn Genomics、下一代测序和生物信息学核心相结合，提供 Illumina 下一代测序作为核心服务。最终数据管理和分析将变得可访问 通过项目调查员受密码保护的共享网络浏览器向每个项目调查员提供信息。 拥有下一代测序 (NGS)、染色质免疫沉淀和生物信息学各个方面的专业知识 将在奖项有效期内为这些项目提供支持。 NGS 核心将以经验为主导 行政和技术专长方面的领导者，以确保核心项目取得成功。利伯曼博士是 NGS 专家，熟悉 NGS 技术的各个方面。罗伯逊博士领导了许多大型 复杂的项目，并熟悉管理和协调所需的技术专业知识 成功。因此，领导层熟悉不同的 NGS 方法和生物信息学分析，例如 应用于致癌疱疹病毒和人类基因组。核心 C 领导者都提供独特的 专业知识相辅相成。 NGS Core C 将提高该计划所有成员的能力 标准化样品制备和测序后生物信息学分析的方法。该工作将 在佩雷尔曼医学院毗邻罗伯逊实验室的空间进行，并结合 与宾夕法尼亚基因组 NGS 核心和生物信息学核心部分由艾布拉姆森癌症支持 中心。