Botswana-UPenn: Research Consortium of HPV-Related Cervical Cancer in HIV Patient

博茨瓦纳-宾夕法尼亚大学：HIV 患者 HPV 相关宫颈癌研究联盟

• 批准号: 10834480
• 负责人: ERLE S. ROBERTSON
• 金额: $ 6.93万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10834480
• 关键词: Acquired Immunodeficiency Syndrome; Botswana; Cancer Burden; Cells; Cervical; Cervix carcinoma; Communicable Diseases; Development; Genetic Transcription; Goals; HIV; HIV Seronegativity; HIV Seropositivity; High Risk Woman; High-Throughput Nucleotide Sequencing; Human; Human Papillomavirus; Incidence; Infectious Agent; Inflammatory Response; Link; Malignant Neoplasms; Malignant neoplasm of cervix uteri; Merkel Cells; Merkel cell carcinoma; Microarray Analysis; Monitor; Natural History; Nucleic Acids; Oncogenic; Pathogenesis; Patients; Polyomavirus; Population; Prevention; Process; Recovery; Research; Technology; Tissues; Validation; Woman; cohort; combinatorial; high risk; improved; low and middle-income countries; response

Abstract: The cervical cancer burden in low and middle income countries make up about 80% of the total cervical cancer incidence worldwide. In Botswana the link between increased incidences of cervical cancer in HIV positive women has not been carefully explored. Furthermore there is an increased link between infectious disease and cancer as seen by additional recent studies including the presence of Merkel Cell Carcinomas with Merkel Cell Polyoma virus. This underscores the importance of these agents and their contributions to the oncogenic process. Greater than 20% of human cancers are associated with infectious agents and that estimate is likely to be lower than the actual number. The advances in technology and approaches which include recovery of nucleic acids from tissues and other materials has greatly improved our ability to detect and confirm the identifications of these agents in associated cancers. The overall goal of the proposed studies in project #1 is to determine the contributions of infectious agents to development of cervical carcinomas in HIV positive women in Botswana. This dramatic increase in cervical cancer incidence in HIV positive women in Botswana allows for the potential contributions of additional agents as well as the inflammatory response which together leads to a more rapid and proliferative response. Our project will take advantage of our unique cohorts of women in Bostwana that allows us to monitor changes in the cervical flora from HPV and HIV negative women to women with cervical cancer who are HIV and HPV positive. Our initial question will be to identify additional agents which may be linked to development of cervical carcinomas in this population. Our working hypothesis is that multiple agents can contribute to the development of cervical carcinomas in HIV positive women in Botswana and this can result in an inflammatory response which synergistically leads to cervical carcinomas. We will utilize our newly developed combinatorial strategy which bridges microarray technology with high throughput sequencing. The identification of these agents will be followed by validation of the presence of these agents in tissues, transcription analysis of infected cells as well as analysis of the innate and adaptive inflammatory responses with the associated agents, and investigating the proliferative effects on these cells.

抽象的： 低收入和中等收入国家的宫颈癌负担约占宫颈癌总数的80% 全球癌症发病率。在博茨瓦纳，宫颈癌发病率增加之间的联系 艾滋病毒阳性女性尚未得到仔细研究。此外，两者之间的联系也有所增加 最近的其他研究表明传染病和癌症，包括默克尔细胞的存在 默克尔细胞多瘤病毒引起的癌症。这强调了这些代理及其作用的重要性 对致癌过程的贡献。超过 20% 的人类癌症与 传染源，估计数可能低于实际数字。的进展 包括从组织和其他材料中回收核酸的技术和方法 极大地提高了我们检测和确认这些药物在相关癌症中的鉴定能力。 项目#1 中拟议研究的总体目标是确定传染源的贡献 博茨瓦纳艾滋病毒阳性妇女患宫颈癌的风险。这一急剧增加 博茨瓦纳艾滋病毒阳性妇女的宫颈癌发病率可能有助于 其他药物以及炎症反应共同导致更快和 增殖反应。我们的项目将利用博茨瓦纳独特的女性群体， 使我们能够监测从 HPV 和 HIV 阴性女性到患有 HPV 和 HIV 阴性女性的宫颈菌群变化 HIV 和 HPV 阳性的宫颈癌患者。我们最初的问题是确定其他代理人 这可能与该人群中宫颈癌的发生有关。我们的工作假设是 多种药物可能导致艾滋病毒阳性女性患宫颈癌 博茨瓦纳，这可能导致炎症反应，协同导致宫颈癌 癌。我们将利用我们新开发的组合策略来桥接微阵列 高通量测序技术。鉴定这些代理人之后将 验证组织中这些物质的存在、受感染细胞的转录分析以及 分析相关药物的先天性和适应性炎症反应，并调查 对这些细胞的增殖作用。

项目成果

Characterization of HPV subtypes in invasive cervical cancer in Botswana patients using a pan-pathogen microarray technology.

使用泛病原体微阵列技术表征博茨瓦纳患者浸润性宫颈癌中的 HPV 亚型。

• 发表时间: 2023-06
• 影响因子: 4.3
• 作者: Grover, Surbhi;Seckar, Tyler;Gao, Le;Bhatia, Rohini;Lin, Xiang;Zetola, Nicola;Ramogola;Robertson, Erle
• 通讯作者: Robertson, Erle

Patient Factors Associated With Delays in Obtaining Cancer Care in Botswana.

博茨瓦纳与延迟获得癌症护理相关的患者因素。

• 发表时间: 2018-08
• 作者: Bhatia, Rohini K;Rayne, Sarah;Rate, William;Bakwenabatsile, Lame;Monare, Barati;Anakwenze, Chidinma;Dhillon, Preet;Narasimhamurthy, Mohan;Dryden;Grover, Surbhi
• 通讯作者: Grover, Surbhi

Gynecologic Cancer: New and Follow-Up Patient Appointments in Botswana During the COVID-19 Pandemic.

妇科癌症：COVID-19 大流行期间博茨瓦纳的新患者和后续患者预约。

• 发表时间: 2021
• 影响因子: 4.5
• 作者: Davey, Sonya;Bazzett;Monare, Barati;Seiphetleng, Alexander;Ramontshonyana, Gaobakwe;Vuylsteke, Peter;Chiyapo, Sebathu;Luckett, Rebecca;Ramogola;Grover, Surbhi
• 通讯作者: Grover, Surbhi

Annotation-free pathology images characterize tumor immune microenvironment in breast cancer by weakly supervised contrastive learning

无注释病理图像通过弱监督对比学习表征乳腺癌的肿瘤免疫微环境

• DOI: 10.1097/00004836-200208000-00001
• 发表时间: 2024-09-13
• 期刊: Journal of Clinical Gastroenterology
• 影响因子: 2.9
• 作者: Guobang Yu;Hui Liu
• 通讯作者: Hui Liu

Pilot of an International Collaboration to Build Capacity to Provide Gynecologic Oncology Surgery in Botswana.

博茨瓦纳妇科肿瘤手术能力建设国际合作试点。

• 发表时间: 2018-11
• 作者: Luckett, Rebecca;Kalenga, Kitenge;Liu, Fong;Esselen, Katharine;Awtrey, Chris;Mmalane, Mompati;Moloi, Thabo;Ricciotti, Hope;Grover, Surbhi
• 通讯作者: Grover, Surbhi