Core D: Chemistry and Analytical Core (CAC)

核心 D：化学和分析核心 (CAC)

• 批准号: 10570856
• 负责人: Kun Lu
• 金额: $ 14.49万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-02-20 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10570856
• 关键词: 16S ribosomal RNA sequencing; Area; Arsenic; Bile Acids; Bioinformatics; Biological; Biological Markers; Chemistry; Communities; Consultations; Data; Data Analyses; Data Set; Data Storage and Retrieval; Detection; Diabetes Mellitus; Diet; Education; Elements; Environmental Science; Equipment; Exposure to; Generations; Goals; Health; High Pressure Liquid Chromatography; Human Resources; Inductively Coupled Plasma Mass Spectrometry; Infrastructure; Institution; Knowledge; Leadership; Link; Mass Spectrum Analysis; Measurement; Measures; Metabolic; Metabolic dysfunction; Metagenomics; Metals; Methodology; Methods; MicroRNAs; Mission; Obesity; Oxidative Stress; Play; Poison; Postdoctoral Fellow; Program Research Project Grants; Protocols documentation; Public Health; Research; Research Methodology; Research Personnel; Research Project Grants; Research Support; Resolution; Sampling; Scientist; Services; Shotguns; Signal Recognition Particle; Students; Superfund; Training; Vision; Work; absorption; acid stress; analytical method; biomarker development; community engagement; complex data; data management; disorder prevention; experience; forging; graduate student; gut microbiome; host microbiome; innovation; instrumentation; metabolomics; microbiome; microbiome alteration; microbiome analysis; microbiome sequencing; next generation; novel; novel strategies; post-doctoral training; prevent; programs; public health relevance; superfund chemical; supportive environment; targeted biomarker; tool; toxicant; transcription factor

ABSTRACT: CHEMISTRY AND ANALYICAL CORE The Chemistry and Analytical Core (CAC) is a critical research support core for the UNC-SRP, providing expertise and analytical capabilities to measure toxic substances with prioritization of inorganic arsenic (iAs) and co-occurring contaminants and characterize their effects on metabolic dysfunction/diabetes. The CAC offers analytical expertise and services, access to state-of-the-art instrumentation for metals measurements, microbiome and biomarker/metabolomics analysis, and training in cutting-edge research methodologies. The CAC provides Projects 1-5 with the tools and expertise needed to generate critical data sets that will help to identify novel strategies to reduce iAs exposure and to prevent iAs-induced metabolic dysfunction. The CAC staff accomplishes tasks through utilization of a versatile Agilent inductively coupled plasma mass spectrometry (ICP-MS) and a hydride generation-cryotrapping-atomic absorption spectrometer (HG-CT-AAS) for total element and speciation analysis, highly sensitive AB Sciex and Thermo TSQ triple quadrupole MS for biomarker quantitation, and high resolution Thermo QE HF Orbitrap MS for metabolomics profiling and metabolite identification. In addition, the CAC has established expertise in microbiome analysis via 16S rRNA gene sequencing and shotgun metagenomics. The overall objective of the Chemistry and Analytical Core is to provide the UNC-SRP Projects with analytical infrastructure, instrumentation, analytical service and related training. Three specific aims will be pursued, including: Aim 1. Develop and implement analytical methodologies, with a focus on separation, detection and quantitation of metals and their metabolites/species; Aim 2. Examine toxicant-induced gut microbiome perturbation and metabolic disruption through metagenomics and metabolomics; and Aim 3. Provide consultation to Biomedical and Environmental Science Projects and a supportive environment for graduate student and postdoctoral training on state-of-the-art analytical equipment. The CAC supports the overall theme of the UNC-SRP: “Identifying novel methods to reduce iAs exposure and elucidating mechanisms underlying iAs-induced metabolic dysfunction with a vision for disease prevention.” !The CAC activities promote interaction among all Projects and Cores through expertise in metals analyses, microbiome and biomarker/metabolomics analysis that is critical in guiding the Projects’ research strategies. With every component in place, including state-of-the-art equipment, rigorous QA/QC plans, highly experienced personnel and a strong leadership team and institutional support, the CAC will play a key role in forging links among the Center researchers. !

摘要：化学和分析核心 化学和分析核心（CAC）是UNC-SRP的关键研究支持核心，提供 通过优先考虑无机砷（IAS）和 共同发生的污染物，并表征其对代谢功能障碍/糖尿病的影响。 CAC提供 分析专业知识和服务，获得金属测量的最先进仪器， 微生物组和生物标志物/代谢组学分析以及尖端研究方法的培训。 CAC为1-5提供了生成关键数据集所需的工具和专业知识，这将有助于 确定减少IAS暴露并预防IAS诱导的代谢功能障碍的新型策略。 CAC 员工通过利用多功能敏捷的电感耦合等离子体质谱法完成任务 （ICP-MS）和用于总元素的水力发电晶状原子 - 原子滥用光谱仪（HG-CT-AAS） 和规格分析，高度敏感的AB Sciex和Thermo TSQ三倍四倍体MS 定量和高分辨率Thermo HF Orbitrap MS用于代谢组学和代谢物 鉴别。此外，CAC通过16S rRNA基因建立了微生物组分析方面的专业知识 测序和shot弹枪宏基因组学。化学和分析核心的总体目标是 向UNC-SRP项目提供分析基础架构，仪器，分析服务和 相关培训。将追求三个具体目标，包括：目标1。开发和实施分析 方法着重于分离金属及其代谢物/物种的分离，检测和定量； AIM 2。检查毒物引起的肠道微生物组扰动和通过宏基因组学的代谢破坏 和代谢组学；和目标3。向生物医学和环境科学项目提供咨询和 研究生和最先进分析设备的博士后培训的支持环境。 CAC支持UNC-SRP的整体主题：“识别减少IAS暴露和的新方法 阐明IAS诱导的代谢功能障碍的基础机制具有预防疾病的愿景。”！ CAC活动通过金属分析中的专业知识来促进所有项目和核心之间的互动， 微生物组和生物标志物/代谢组学分析对于指导项目的研究策略至关重要。 每个组件都到位，包括最先进的设备，严格的QA/QC计划，经验丰富 人事和强大的领导团队和机构支持，CAC将在建立链接中发挥关键作用 在中心研究人员中。 呢